广元市白纹伊蚊电压门控钠离子通道I1532T突变频率调查分析

doi:10.12140/j.issn.1000-7423.2026.03.019

中国寄生虫学与寄生虫病杂志 ›› 2026, Vol. 44 ›› Issue (3): 443-447.doi: 10.12140/j.issn.1000-7423.2026.03.019

广元市白纹伊蚊电压门控钠离子通道I1532T突变频率调查分析

逯小强()(), 杨滨瑜, 赵琼瑶, 孔新明, 刘艳春, 贾永朝^*()()

广元市疾病预防控制中心传染病防制所，四川广元 628040

收稿日期:2025-09-03 修回日期:2025-11-10 出版日期:2026-06-30 发布日期:2026-05-21
通讯作者: *贾永朝（ORCID：0009-009-0011-0027），男，本科，主任医师，主要从事传染性疾病预防控制工作。E-mail：452164352@qq.com
作者简介:逯小强（ORCID：0009-0009-3696-7778），男，硕士研究生，助理研究员，主要从事病媒生物防制工作。E-mail：540714749@qq.com
第一联系人：作者贡献
逯小强负责实验设计、实验操作、数据分析、论文撰写，杨滨瑜和赵琼瑶负责样品采集和实验操作，刘艳春和孔新明负责实验准备，贾永朝负责结果核验和论文修改。
基金资助:
广元市社会发展领域重点研发项目(21ZDYF0115)

Frequency of I1532T mutations in the voltage-gated sodium channel of Aedes albopictus in Guangyuan City

LU Xiaoqiang()(), YANG Binyu, ZHAO Qiongyao, KONG Xinming, LIU Yanchun, JIA Yongchao^*()()

Institute for Communicable Disease Control and Prevention, Guangyuan Center for Disease Control and Prevention, Guangyuan 628040, Sichuan, China

Received:2025-09-03 Revised:2025-11-10 Online:2026-06-30 Published:2026-05-21
Contact: *E-mail：452164352@qq.com
Supported by:
Key Research and Development Project in the Field of Social Development of Guangyuan City(21ZDYF0115)

摘要/Abstract

摘要：

为了解四川省广元市白纹伊蚊电压门控钠离子通道（vgsc）基因I1532T突变频率，于2024年6—7月在广元市7个县（区）居民区外以CO₂灯诱法采集白纹伊蚊成蚊，提取基因组DNA，聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）检测白纹伊蚊vgsc 1532位点的基因型。通过测序样品验证PCR-RFLP技术的检测效果。使用Genepop 4.7进行种群哈代温伯格平衡（HWE）检验。使用SPSS 25.0对各县（区）间等位基因频率进行Fisher精确检验。共检测白纹伊蚊208只。PCR结果显示，所有样品均扩增出约220 bp的条带，与预期大小相符。广元市vgsc野生型1532I/I纯合子、野生型/突变型1532I/T杂合子、抗性1532T/T纯合子频率分别为87.98%（183/208）、10.58%（22/208）和1.44%（3/208）。7个县（区）中，剑阁县、利州区、青川县、旺苍县、昭化区野生型/突变型1532I/T杂合子频率分别为12.50%（4/32）、9.38%（3/32）、17.86%（5/28）、28.13%（9/32）、1/16，朝天区和苍溪县未发现；利州区和旺苍县抗性1532T/T纯合子频率分别为6.25%（2/32）和3.13%（1/32），其他县（区）未发现。广元市vgsc野生型1532I和突变型1532T等位基因的频率分别为93.27%（388/416）、6.73%（28/416）。朝天区、苍溪县、剑阁县、利州区、青川县、旺苍县和昭化区突变型1532T等位基因频率分别为0（0/64）、0（0/72）、6.25%（4/64）、10.94%（7/64）、8.93%（5/56）、17.19%（11/64）、3.13%（1/32），差异有统计学意义（P < 0.05）。HWE检验显示，利州区种群显著偏离遗传平衡（P < 0.05），剑阁县、青川县和旺苍县种群均符合遗传平衡（P > 0.05），其余3个县（区）因未发现1532T突变（朝天区和苍溪县）或突变频率低（昭化区），不适合做HWE检验。验证结果显示，PCR-RFLP与Sanger测序结果符合率100%。本研究发现广元市白纹伊蚊种群vgsc I1532T突变频率较高，并在利州区和旺苍县检测到抗性纯合子个体。

关键词: 白纹伊蚊, 聚合酶链式反应-限制性片段长度多态性, 电压门控钠离子通道, 击倒抗性突变

Abstract:

To investigate the frequency of the I1532T mutation in the voltage-gated sodium channel (vgsc) of Aedes albopictus in Guangyuan City, Sichuan Province, adult Ae. albopictus mosquitoes were collected using CO₂-baited light trapsfrom residential areas in seven counties (districts) of Guangyuan City between June and July in 2024. Genomic DNA was extracted from mosquitoes, and the genotype at the vgsc 1532 locus was detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The detection performance of PCR-RFLP assay was validated by sequencing. Hardy-Weinberg equilibrium (HWE) test for populations was assessed using the software Genepop 4.7, and Fisher’s exact test was used to compare allele frequencies across counties (districts) using the software SPSS 25. A total of 208 Ae. albopictus mosquitoes were detected. PCR amplification of all samples showed bands with approximately 220 bp in length, which was consistent with the expected size. The frequencies of the vgsc wild-type homozygous 1532I/I genotype, wild-type/mutant heterozygous 1532I/T genotype, and resistant homozygous 1532T/T genotype were 87.98% (183/208), 10.58% (22/208), and 1.44% (3/208) in Guangyuan City, respectively. Among 7 counties (districts), the frequencies of wild-type/mutant heterozygous 1532I/T genotype were 12.5% (4/32), 9.38% (3/32), 17.86% (5/28), 28.13% (9/32) and 1/16 in Jiange County, Lizhou District, Qingchuan County, Wangcang County and Zhaohua District, respectively, while no heterozygous genotype were found in Chaotian District or Cangxi County. The frequencies of resistant homozygous 1532T/T genotype were 6.25% (2/32) and 3.13% (1/32) in Lizhou District and Wangcang County, respectively, and this homozygous genotype were not detected in other counties (districts). The frequencies of the vgsc wild-type 1532I and mutant 1532T alleles were 93.27% (388/416) and 6.73% (28/416) in Guangyuan City, respectively, and the frequencies of mutant 1532T alleles were 0 (0/64), 0 (0/72), 6.25% (4/64), 10.94% (7/64), 8.93% (5/56), 17.19% (11/64), and 3.13% (1/32) in Chaotian District, Cangxi County, Jiange County, Lizhou District, Qingchuan County, Wangcang County, and Zhaohua District, respectively (P < 0.05). HWE test showed that the Ae. albopictus population in Lizhou District deviated significantly from equilibrium (P < 0.05), and Ae. albopictus populations from Jiange County, Qingchuan County, and Wangcang County were at equilibrium (P > 0.05), while Ae. albopictus populations from other three counties (districts) were not suitable for HWE tests due to absence of the 1532T mutation (Chaotian District and Cangxi County) or low mutation frequency (Zhaohua District). The verification results showed that the coincidence rate between PCR-RFLP and sanger sequencing results was 100%. This study indicates a relatively high frequency of the vgsc I1532T mutation in Ae. albopictus populations from Guangyuan City, with resistant homozygotes detected in Lizhou District and Wangcang County.

Key words: Aedes albopictus, Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), Voltage-gated sodium channel, Knockdown resistance mutation

中图分类号:

R384.1

逯小强, 杨滨瑜, 赵琼瑶, 孔新明, 刘艳春, 贾永朝. 广元市白纹伊蚊电压门控钠离子通道I1532T突变频率调查分析[J]. 中国寄生虫学与寄生虫病杂志, 2026, 44(3): 443-447.

LU Xiaoqiang, YANG Binyu, ZHAO Qiongyao, KONG Xinming, LIU Yanchun, JIA Yongchao. Frequency of I1532T mutations in the voltage-gated sodium channel of Aedes albopictus in Guangyuan City[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2026, 44(3): 443-447.

图/表 2

参考文献 22

[1]	Smith LB, Kasai S, Scott JG. Pyrethroid resistance in Aedes aegypti and Aedes albopictus: important mosquito vectors of human diseases[J]. Pestic Biochem Physiol, 2016, 133: 1-12.
[2]	Pichler V, Mancini E, Micocci M, et al. A novel allele specific polymerase chain reaction (AS-PCR) assay to detect the V1016G knockdown resistance mutation confirms its widespread presence in Aedes albopictus populations from Italy[J]. Insects, 2021, 12(1): 79.
[3]	张曼曼, 罗正汉, 王春芳, 等. 2005—2020年中国登革热发病趋势及年龄-时期-队列分析[J]. 中华卫生杀虫药械, 2025, 31(2): 136-141.
	Zhang MM, Luo ZH, Wang CF, et al. Analysis on the incidence trend and age-period-cohort of dengue fever in China from 2005 to 2020[J]. Chin J Hyg Insectic Equip, 2025, 31(2): 136-141. (in Chinese)
[4]	Gratz NG. Critical review of the vector status of Aedes albopictus[J]. Med Vet Entomol, 2004, 18(3): 215-227.
[5]	杨舒然, 刘起勇. 白纹伊蚊的全球分布及扩散趋势[J]. 中国媒介生物学及控制杂志, 2013, 24(1): 1-4.
	Yang SR, Liu Q. Trend in global distribution and spread of Aedes albopictus[J]. Chin J Vector Biol Control, 2013, 24(1): 1-4. (in Chinese)
[6]	Zhao QY, Jia YC, Lu XQ, et al. Insecticide susceptibility and KDR mutations in Aedes albopictus collected from seven districts of Guangyuan City, northern Sichuan, China[J]. J Am Mosq Control Assoc, 2024, 40(1): 20-25.
[7]	罗兴, 贾永朝, 刘艳春, 等. 四川省广元市2011—2020年自然疫源和虫媒传染病流行特征[J]. 中国热带医学, 2021, 21(11): 1093-1097.
	Luo X, Jia YC, Liu YC, et al. Epidemiological characteristics of natural focus and vector-borne infectious diseases in Guangyuan, Sichuan, 2011-2020[J]. Chin Trop Med, 2021, 21(11): 1093-1097. (in Chinese)
[8]	Kasai S, Caputo B, Tsunoda T, et al. First detection of a Vssc allele V1016G conferring a high level of insecticide resistance in Aedes albopictus collected from Europe (Italy) and Asia (Vietnam), 2016: a new emerging threat to controlling arboviral diseases[J]. Eur Commun Dis Bull, 2019, 24(5): 1700847.
[9]	赵春春, 周欣欣, 李文玉, 等. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 30-37.
	Zhao CC, Zhou XX, Li WY, et al. Insecticide resistance surveillance and characteristic analysis of dengue vector Aedes albopictus in 13 provinces of China in 2020[J]. Chin J Vector Biol Control, 2022, 33(1): 30-37. (in Chinese)
[10]	Zhou XJ, Yang C, Liu N, et al. Knockdown resistance (kdr) mutations within seventeen field populations of Aedes albopictus from Beijing China: first report of a novel V1016G mutation and evolutionary origins of kdr haplotypes[J]. Parasit Vectors, 2019, 12(1): 180.
[11]	王纯玉, 赵春春, 邢俊, 等. 辽宁省白纹伊蚊对3种拟除虫菊酯类杀虫剂的抗药性及其击倒抗性基因型分布研究[J]. 中国媒介生物学及控制杂志, 2025, 36(1): 22-27.
	Wang CY, Zhao CC, Xing J, et al. Resistance of Aedes albopictus to three pyrethroid insecticides and the distribution of their knockdown resistance genotypes in Liaoning Province, China[J]. Chin J Vector Biol Control, 2025, 36(1): 22-27. (in Chinese)
[12]	Qiu XH, Pan J, Li M, et al. PCR-RFLP methods for detection of insecticide resistance-associated mutations in the house fly (Musca domestica)[J]. Pestic Biochem Physiol, 2012, 104(3): 201-205.
[13]	刘鹃, 刘鹏, 王雅伟, 等. 白纹伊蚊和三带喙库蚊抗药性相关基因突变PCR-RFLP检测方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(6): 744-747, 755.
	Liu J, Liu P, Wang YW, et al. Establishment of a PCR-RFLP method for detecting mutations in genes related to insecticide resistance in Aedes albopictus and Culex tritaeniorhynchus[J]. Chin J Parasitol Parasit Dis, 2024, 42(6): 744-747, 755. (in Chinese)
[14]	董血书, 周红宇, 龚正达. 云南蚊类志·下卷[M]. 昆明: 云南科技出版社, 2010: 81-82.
	Dong XS, Zhou HY, Gong ZD. Mosquito Fauna of Yunnan, VolumeⅡ[M]. Kunming: Yunnan Science and Technology Press, 2010: 81-82. (in Chinese)
[15]	Mu QZ, Zhao X, Li FF, et al. A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology[J]. Infect Dis Poverty, 2023, 12: 74.
[16]	张龙飞, 张晓越, 高翔. 核酸质谱检测白纹伊蚊击倒抗性基因突变方法的建立[J]. 中华卫生杀虫药械, 2025, 31(3): 266-269.
	Zhang LF, Zhang XY, Gao X. Establishment of nucleic acid mass spectrometry for detection of knockdown resistance gene mutations in Aedes albopictus[J]. Chin J Hyg Insectic Equip, 2025, 31(3): 266-269. (in Chinese)
[17]	Zhu CY, Zhao CC, Wang YG, et al. Establishment of an innovative and sustainable PCR technique for 1534 locus mutation of the knockdown resistance (kdr) gene in the dengue vector Aedes albopictus[J]. Parasit Vectors, 2019, 12(1): 603.
[18]	Pichler V, Valadas V, Akiner MM, et al. Tracking pyrethroid resistance in arbovirus mosquito vectors: mutations I1532T and F1534C in Aedes albopictus across Europe[J]. Parasit Vectors, 2025, 18(1): 506.
[19]	周秋明, 盛望望, 李佳璇, 等. 上海市2024年白纹伊蚊击倒抗性基因突变检测[J]. 中国热带医学, 2025, 25(11): 1446-1450, 1498.
	Zhou QM, Sheng WW, Li JX, et al. A surveillance study of knockdown resistance gene mutations in Aedes albopictus from Shanghai in 2024[J]. China Trop Med, 2025, 25(11): 1446-1450, 1498. (in Chinese)
[20]	谭爱, 刘鹃, 王雅伟, 等. 四川省内江市白纹伊蚊野外群体电压门控钠离子通道基因突变检测分析[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 314-318.
	Tan A, Liu J, Wang YW, et al. Detection of mutations in the voltage-gated sodium channel genes of field Aedes albopictus populations in Neijiang, Sichuan Province, China[J]. Chin J Vector Biol Control, 2023, 34(3): 314-318. (in Chinese)
[21]	朱彩英, 赵春春, 王纯玉, 等. 湖南省长沙市内五区白纹伊蚊电压门控钠离子通道基因突变研究[J]. 中国媒介生物学及控制杂志, 2024, 35(4): 405-410.
	Zhu CY, Zhao CC, Wang CY, et al. A study on voltage-gated sodium channel gene mutations in Aedes albopictus in five urban districts of Changsha, Hunan Province, China[J]. Chin J Vector Biol Control, 2024, 35(4): 405-410. (in Chinese)
[22]	母群征, 华栋栋, 李文玉, 等. 河南省禹州地区白纹伊蚊击倒抗性基因突变检测[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 303-307, 335.
	Mu QZ, Hua DD, Li WY, et al. Detection of knockdown resistance gene mutations in Aedes albopictus in Yuzhou, Henan Province, China[J]. Chin J Vector Biol Control, 2023, 34(3): 303-307, 335. (in Chinese)

区（县）	数量	基因型蚊虫数（频率/%）
区（县）	数量	野生型I/I	野生型/突变型I/T	抗性T/T
朝天区	32	32（100）	0（0）	0（0）
苍溪县	36	36（100）	0（0）	0（0）
剑阁县	32	28（87.50）	4（12.50）	0（0）
利州区	32	27（84.38）	3（9.38）	2（6.25）
青川县	28	23（82.14）	5（17.86）	0（0）
旺苍县	32	22（68.75）	9（28.13）	1（3.13）
昭化区	16	15（15/16）	1（1/16）	0（0）
合计	208	183（87.98）	22（10.58）	3（1.44）

广元市白纹伊蚊电压门控钠离子通道I1532T突变频率调查分析

Frequency of I1532T mutations in the voltage-gated sodium channel of Aedes albopictus in Guangyuan City

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 22

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王丹, 张燕, 喻涛洪, 谢朋亮, 周敬祝, 师伟芳, 罗小龙, 梁文琴. 贵州省安顺市白纹伊蚊对拟除虫菊酯类杀虫剂的抗性机制研究[J]. 中国寄生虫学与寄生虫病杂志, 2026, 44(3): 393-401.
[2]	苏媛馨, 蔡京虹, 尹紫鸽, 刘庆, 郑耀婕, 郭澳, 姚二明, 张惠雯, 赵彤言, 张恒端, 邓长生. 诺卡酮对白纹伊蚊的驱避效果研究[J]. 中国寄生虫学与寄生虫病杂志, 2025, 43(5): 651-655.
[3]	刘鹃, 刘鹏, 王雅伟, 余小梅, 邱星辉. 白纹伊蚊和三带喙库蚊抗药性相关基因突变PCR-RFLP检测方法的建立[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(6): 744-747.
[4]	王冠熙, 李雅姝, 李月月, 曹园园, 杨蒙蒙, 张梅花, 吴竞瑶, 梁成, 李菊林, 周华云, 唐建霞, 朱国鼎. 江苏省白纹伊蚊对溴氰菊酯抗性及击倒抗性突变分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 468-474.
[5]	夏丹，滕萍英，陈晓光，周晓红*. 白纹伊蚊滞育及其分子机制[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(3): 18-282-289.
[6]	李星潘, 曹国梅, 邢翠翠, 华倩倩, 张亮亮, 梁韶晖. 白纹伊蚊唾液腺重组aegyptin相似蛋白alALP的制备及抗原性分析[J]. 中国寄生虫学与寄生虫病杂志, 2014, 32(3): 6-193-197.
[7]	刘鉴1，程金芝1，孙宇2，朱儒芳3，张亮4，吴家红1 *. 白纹伊蚊唾液腺黏蛋白相关蛋白基因1的克隆、表达分析[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(2): 8-120-123.
[8]	吴松泉1 *，王光丽1，雷永良2，张凯波1. 白纹伊蚊唾液三磷酸腺苷二磷酸酶在毕赤酵母中的分泌表达[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(1): 19-73-75.
[9]	蔡燕丽，郑学礼*. 白纹伊蚊AGO2和Dcr-2基因片段克隆及各发育期转录水平分析[J]. 中国寄生虫学与寄生虫病杂志, 2012, 30(3): 10-214-217.
[10]	吴家红;程金芝;陈璐;孙宇. 腺苷脱氨酶、C型凝集素及丝氨酸蛋白酶抑制剂基因在白纹伊蚊唾液腺中的表达[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(3): 7-193.
[11]	张玲敏;孙家梅;吕慧芳;施海瑛. α-三噻吩对白纹伊蚊幼虫蛋白质、酯酶及脂质过氧化的影响[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(1): 11-49.
[12]	顾金保;孙彦涛;彭鸿娟. 白纹伊蚊蜕皮激素拮抗剂筛选酵母模型的建立[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(5): 5-380.
[13]	吴家红;赵彤言;董言德. 溴氰菊酯抗性白纹伊蚊抑制消减cDNA文库的构建[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(4): 10-284.
[14]	任晓燕;张玲敏;朱佩娴;熊钟谨;吴春云. α-三噻吩对白纹伊蚊幼虫的毒杀作用[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(5): 12-299.
[15]	牟荣;包怀恩;李建华. 感染大链壶菌后蚊幼虫脂肪、酯酶和脂肪酶的变化[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(5): 7-282.