犬弓首蛔虫卵体外发育的形态观察

doi:10.12140/j.issn.1000-7423.2019.04.020

中国寄生虫学与寄生虫病杂志 ›› 2019, Vol. 37 ›› Issue (4): 486-489.doi: 10.12140/j.issn.1000-7423.2019.04.020

犬弓首蛔虫卵体外发育的形态观察

张艺琳(), 张森棹, 古小彬, 杨光友, 谢跃^*()

四川农业大学动物医学院动物寄生虫病研究中心,成都 611130

收稿日期:2019-07-02 出版日期:2019-08-30 发布日期:2019-09-05
通讯作者: 谢跃
作者简介:
作者简介：张艺琳（1997-）,女,本科,主要从事动物寄生虫病学研究。E-mail：zyl210young@163.com
基金资助:
四川省国际科技创新合作/港澳台科技创新合作项目（No. 2019YFH0065）

Morphological observations of Toxocara canis egg development in vitro

Yi-lin ZHANG(), Sen-zhao ZHANG, Xiao-bin GU, Guang-you YANG, Yue XIE^*()

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2019-07-02 Online:2019-08-30 Published:2019-09-05
Contact: Yue XIE
Supported by:
Supported by the Sichuan International Science and Technology Innovation Cooperation/Hong Kong, Macao and Taiwan Science and Technology Innovation Cooperation Project (No. 2019YFH0065)

摘要/Abstract

摘要：

为了研究犬弓首蛔虫卵的体外发育形态学变化,采用显微自动拍照系统对体外培养的犬弓首蛔虫卵进行了连续21 d观察和记录。结果表明,犬弓首蛔虫卵存在12个体外发育阶段,即1细胞期、2细胞期、3细胞期、4细胞期、早期桑葚期、晚期桑葚期、囊胚期、肠胚期、1期幼虫前期、1期幼虫期、2期幼虫前期和2期幼虫期。观察发现,虫卵在培养24 h后开始出现卵裂,形成2细胞期虫卵;虫卵第3~6天陆续发育形成3~4细胞期、桑葚期、囊胚期以及肠胚期虫卵;第7天,22.5%虫卵发育形成1期幼虫,并表现出一定趋光特性;第8天,10.67%虫卵发育形成感染性2期幼虫;第17天,感染性2期幼虫从虫卵孵出,卵壳塌陷。这些结果补充和完善了犬弓首蛔虫卵的体外发育形态变化资料。

关键词: 犬弓首蛔虫, 虫卵, 发育, 形态学变化

Abstract:

To observe the morphological changes of Toxocara canis egg development, an automatic microscopic photographing system was setup to track and record the whole process of T. canis egg development during continuous 21-day egg culture in vitro in this study. The results demonstrated that the whole development of T. canis egg in vitro was divided into 12 developmental stages including one-cell, two-cell, three-cell, four-cell, early-morula, late-morula, blastula, gastrula, pre-larva, L1 (the first stage larva), pre-L2 and L2 stages. The cell started to divide to form two-cell egg in 24 hours culture, appeared as 3-4 cells egg, morula, blastula and gastrula stages in 3-6 days. Total 22.5% of eggs developed to embryonated L1 with phototactic movements in 7 days. Total 10.67% eggs developed to infective embryonated L2 in 8 days. At 17-day post culture, the infective larva have been hatched and the whole eggshell collapsed. These data provide more information for the developmental biology of T. canis egg in vitro.

Key words: Toxocara canis, Eggs, Development, Morphological changes

中图分类号:

R383.11

张艺琳, 张森棹, 古小彬, 杨光友, 谢跃. 犬弓首蛔虫卵体外发育的形态观察[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(4): 486-489.

Yi-lin ZHANG, Sen-zhao ZHANG, Xiao-bin GU, Guang-you YANG, Yue XIE. Morphological observations of Toxocara canis egg development in vitro[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2019, 37(4): 486-489.

图/表 3

图1

图2

图3

参考文献 23

[1]	Macpherson CNL.The epidemiology and public health importance of toxocariasis: a zoonosis of global importance[J]. Int J Parasitol, 2013, 43(12/13): 999-1008.
[2]	Smith H, Holland C, Taylor M, et al. How common is human toxocariasis? Towards standardizing our knowledge[J]. Trends Parasitol, 2009, 25(4): 182-188.
[3]	Ma G, Holland CV, Wang T, et al. Human toxocariasis[J]. Lancet Infect Dis, 2018, 18(1): e14-e24.
[4]	Jia C, Liu Q, Liu GH, et al. Toxocariasis: a silent threat with a progressive public health impact[J]. Infect Dis Poverty, 2018, 7: 59.
[5]	Wolfe A, Wright PI.Human toxocariasis and direct contact with dogs[J]. Vet Record, 2003, 15(2): 14-25.
[6]	Dickson D.Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects[J]. Clin Microbiol Rev, 2003, 16(2): 265-272.
[7]	李兴荣, 董银秀, 段月辉, 等. 一例人感染犬弓首蛔虫的诊治[J]. 畜禽业, 2009(6): 93-93.
[8]	张海芳, 华海涌. 江苏省首例临床诊断人眼弓首线虫病报告[J]. 热带病与寄生虫学, 2014, 12(2): 104-105.
[9]	Azam D, Ukpai OM, Said A, et al. Temperature and the development and survival of infective Toxocara canis larvae[J]. Parasitol Res, 2012, 110(2): 649-656.
[10]	Camparoto ML, Fulan B, Colli CM, et al. Initial stage of development and migratory behavior of Toxocara canis larvae in BALB/c mouse experimental model[J]. Genet Mol Res, 2008, 7(2): 444-450.
[11]	Gamboa MI.Effects of temperature and humidity on the development of eggs of Toxocara canis under laboratory conditions[J]. J Helminthol, 2005, 79(4): 327-331.
[12]	Cruz LM, Allanson M, Kwa B, et al. Morphological changes of Ascaris spp. eggs during their development outside the host[J]. J Parasitol, 2012, 98(1): 63-69.
[13]	De Savigny DH.In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans[J]. J Parasitol, 1975, 61(4): 781-782.
[14]	Traversa D, Di regalbono AF, Cesare A, et al. Environmental contamination by canine geohelminths[J]. Parasit Vectors, 2014, 7: 67.
[15]	Macpherson CNL.Dogs, zoonoses, and public health[M]. Oxfordshire: Boston, 2012.
[16]	Moudgil AD, Singla LD, Singh MP.In vitro study targeting on developmental embryonation pattern of eggs of ascarid species of wild animals[J]. Appl Biol Res, 2014, 16: 237-241.
[17]	Rodriguez-Caballero A, Luna-Ochoa RI, Ponce-Macotela M, et al. A simple and inexpensive in vitro method for retrieving fertilized Toxocara canis eggs[J]. Parasitol Res, 2007, 101(3): 829-832.
[18]	Hadi A, Jassim S.Laboratory incubation of Toxocara canis eggs and their development[J]. J Bio Env Sci, 2018, 12(2):148-152.
[19]	Brunaskám M, Dubinsky P, Reiterová K.Toxocara canis: ultrastructural aspects of larval moulting in the maturing eggs[J]. Int J Parasitol, 1995, 25(6): 683-690.
[20]	Bowman DD, Oaks JA, Grieve RB.Ultrastructure of the infective stage larva of Toxocara canis (Werner, 1782) Stiles, 1905 (Nematoda : Ascaridoidea)[J]. J Helminthol Soc Washington, 1993, 60: 183-204.
[21]	Thomas D, Jeyathilakan N, Basith SA, et al. In vitro production of Toxocara canis excretory-secretory(TES) antigen[J]. J Parasitic Dis, 2016, 40(3): 1038-1043.
[22]	Abou-El-Naga IF. Developmental stages and viability of Toxocara canis eggs outside the host[J]. Biomédica, 2018, 38(2): 189-197.
[23]	Hiura E, Lopes ACG, da Paz JS, et al. Fungi predatory activity on embryonated Toxocara canis eggs inoculated in domestic chickens (Gallus gallus domesticus) and destruction of second stage larvae[J]. Parasitol Res, 2015, 114(9): 3301-3308.

犬弓首蛔虫卵体外发育的形态观察

Morphological observations of Toxocara canis egg development in vitro

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 23

相关文章 15

编辑推荐

Metrics

本文评价

[1]	汤非凡, 蒋峰, 汪梅青, 王澜, 曹佳诚, 夏钰婷, 王婧璇, 唐礼庆, 湛孝东. 速生薄口螨成螨的形态观察和系统进化分析[J]. 中国寄生虫学与寄生虫病杂志, 2023, 41(3): 344-348.
[2]	王晓玲, 张卫, 易存, 陈祥宇, 杨文彬, 徐斌, 胡薇. SjGPR89蛋白对日本血吸虫生长发育的影响[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(6): 701-707.
[3]	韦开文, 曾红霞, 何牧, 胡俊杰. 浣熊贝氏蛔虫核糖体基因和线粒体基因分析[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(5): 594-602.
[4]	王吉鹏. 驱动血吸虫生长发育的干细胞研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(4): 436-440.
[5]	卓怡呈, 杨海成, 刘程豪, 张宝财, 多小勇, 张示杰. 骨桥蛋白表达水平对多房棘球蚴原头节生长发育的影响[J]. 中国寄生虫学与寄生虫病杂志, 2022, 40(3): 299-304.
[6]	洪炀, 林矫矫. 日本血吸虫蛋白质组学研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(6): 725-730.
[7]	王国英, 陈丹丹, 郑学礼, 李祥会, 张浩, 张军, 滕铁山. 旋毛虫成囊幼虫的感染性与其发育阶段的关系[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(6): 785-788.
[8]	肖丽贞, 谢汉国, 陈朱云, 欧阳榕, 江典伟. 红带锥蝽实验条件下发育特征研究[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(2): 219-223.
[9]	孙钰浚, 李钊琪, 吕芳丽. 日本血吸虫虫卵肉芽肿免疫病理机制研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(6): 713-717.
[10]	曾雪梅, 蒋智华, 申继清, 杨庆利. 广西146例华支睾吸虫疑似感染者粪样虫卵和血清IgG抗体的检测分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(6): 730-732.
[11]	谭磊, 王爱兵, 孔小鲜, 梁轩, 贺峻琳, 李洁, 胡丹, 刘伟. 湖南省不同种类蛇源裂头蚴线粒体pnad1基因序列多态性研究[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(4): 448-452.
[12]	刘欣, 齐永芬, 鱼艳荣. 血吸虫病肝纤维化中可溶性虫卵抗原对肝星状细胞的作用[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(2): 218-222.
[13]	冯婷, 秦志强, 许静, 周杰, 钱颖骏, 祝红庆, 吕山, 曹淳力, 李石柱. 环介导等温扩增法检测粪样中日本血吸虫虫卵DNA的效果评估[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(3): 230-234.
[14]	罗雪平, 袁南贵, 黄俊. 可溶性虫卵抗原和成虫抗原刺激日本血吸虫感染小鼠肠系膜淋巴结Th17细胞的免疫应答[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2): 105-109.
[15]	刘婧, 陈丹, 庄桂芬, 黄振东, 薛志静, 张瑞玲, 张忠. 家蝇发育过程中肠道可培养共生细菌的分离与鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2): 120-124.