中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (4): 290-292.

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中国大陆日本血吸虫品系的研究——Ⅵ.多位点酶电泳分析

何毅勋,李新武,胡亚青,郁琪芳   

  1. 中国预防医学科学院寄生虫病研究所; 中国预防医学科学院流行病学微生物学研究所; 中国预防医学科学院寄生虫病研究所; 中国预防医学科学院寄生虫病研究所
  • 出版日期:1991-11-30 发布日期:2017-01-09
  • 基金资助:
    国家自然科学基金

STUDIES ON STRAIN DIFFERENCES OF SCHISTOSOMA JAPONICUM IN THE MAINLAND OF CHINA VI. ANALYSIS WITH MULTILOCUS ENZYME ELECTROPHORESIS

  • Online:1991-11-30 Published:2017-01-09

摘要: 本文对中国大陆的安徽、湖北、广西、四川、云南5地日本血吸虫的自然隔离群体进行了多位点酶电泳分析。检测了GDH、G6PD、LDH、MDH、PGI、PGM、SOD 7种同工酶的9个基因位点,其中LDH-1、LDH-2、MDH和PGM位点存在两个以上不同等位基因。结果显示多态位点所占的比例为44.4%,说明在大陆境内日本血吸虫的自然隔离群体中存在着不同程度的遗传多态现象。

关键词: 日本血吸虫, 多位点酶电泳, 遗传多态

Abstract: Five different field-collected isolates (i.e., Anhui, Hubei, Guangxi, Sichuan and Yunnan) of Schistosoma japonicum from the mainland of China were compared by means of multi-locus enzyme electrophoresis in polyacrylamide gels of individual male worm extracts. The enzyme systems used in the study included GDH, G6PD, LDH, MDH, PGI, PGM and SOD. Of 9 loci examined in the 7 isozyme systems, 4 were found to be polymorphic. These were LDH-1, LDH-2, MDH and PGM, the remainder of GDH, G6PD-1, G6PD-2, PGI and SOD being monomorphic. Our results indicated that the genetic polymorphism occurring in the natural population of different isolates of S. japonicum in the mainland of China. The allele frequencies at polymorphic loci coding for enzyme as well as the genetic dentity and genetic distance in diffetent isolates of S. japonicum will be reported elsewhere.