作者简介：张艺琳（1997-）,女,本科,主要从事动物寄生虫病学研究。E-mail：
收稿日期: 2019-07-02
网络出版日期: 2019-09-05
基金资助
四川省国际科技创新合作/港澳台科技创新合作项目（No. 2019YFH0065）
Morphological observations of Toxocara canis egg development in vitro
Received date: 2019-07-02
Online published: 2019-09-05
Supported by
Supported by the Sichuan International Science and Technology Innovation Cooperation/Hong Kong, Macao and Taiwan Science and Technology Innovation Cooperation Project (No. 2019YFH0065)
为了研究犬弓首蛔虫卵的体外发育形态学变化,采用显微自动拍照系统对体外培养的犬弓首蛔虫卵进行了连续21 d观察和记录。结果表明,犬弓首蛔虫卵存在12个体外发育阶段,即1细胞期、2细胞期、3细胞期、4细胞期、早期桑葚期、晚期桑葚期、囊胚期、肠胚期、1期幼虫前期、1期幼虫期、2期幼虫前期和2期幼虫期。观察发现,虫卵在培养24 h后开始出现卵裂,形成2细胞期虫卵;虫卵第3~6天陆续发育形成3~4细胞期、桑葚期、囊胚期以及肠胚期虫卵;第7天,22.5%虫卵发育形成1期幼虫,并表现出一定趋光特性;第8天,10.67%虫卵发育形成感染性2期幼虫;第17天,感染性2期幼虫从虫卵孵出,卵壳塌陷。这些结果补充和完善了犬弓首蛔虫卵的体外发育形态变化资料。
张艺琳 , 张森棹 , 古小彬 , 杨光友 , 谢跃 . 犬弓首蛔虫卵体外发育的形态观察[J]. 中国寄生虫学与寄生虫病杂志, 2019 , 37(4) : 486 -489 . DOI: 10.12140/j.issn.1000-7423.2019.04.020
To observe the morphological changes of Toxocara canis egg development, an automatic microscopic photographing system was setup to track and record the whole process of T. canis egg development during continuous 21-day egg culture in vitro in this study. The results demonstrated that the whole development of T. canis egg in vitro was divided into 12 developmental stages including one-cell, two-cell, three-cell, four-cell, early-morula, late-morula, blastula, gastrula, pre-larva, L1 (the first stage larva), pre-L2 and L2 stages. The cell started to divide to form two-cell egg in 24 hours culture, appeared as 3-4 cells egg, morula, blastula and gastrula stages in 3-6 days. Total 22.5% of eggs developed to embryonated L1 with phototactic movements in 7 days. Total 10.67% eggs developed to infective embryonated L2 in 8 days. At 17-day post culture, the infective larva have been hatched and the whole eggshell collapsed. These data provide more information for the developmental biology of T. canis egg in vitro.
Key words： Toxocara canis; Eggs; Development; Morphological changes
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