收稿日期: 2024-06-26
修回日期: 2024-11-12
网络出版日期: 2025-03-26
基金资助
上海市加强公共卫生体系建设三年行动计划（2023—2025年）重点学科项目(GWVI-11.1-12);上海市公共卫生体系建设三年行动计划（2020—2022年）(GWV-10.1-XK13);上海市卫生健康委员会卫生行业临床研究专项青年项目(20214Y0206)
Establishment and influencing factors of experimental animal models for intestinal protozoa infection
Received date: 2024-06-26
Revised date: 2024-11-12
Online published: 2025-03-26
Supported by
Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai (2023-2025) Key Discipline Project(GWVI-11.1-12);Three-Year Public Health Action Plan (2020-2022) of Shanghai Municipal Public Health System Construction(GWV-10.1-XK13);Clinical Research Special Project for Young Scholars in Shanghai Municipal Health Commission(20214Y0206)
章孝成 , 沈玉娟 , 胡媛 , 姜岩岩 , 刘华 , 曹建平 . 肠道原虫实验动物感染模型的建立及其影响因素[J]. 中国寄生虫学与寄生虫病杂志, 2025 , 43(1) : 135 -139 . DOI: 10.12140/j.issn.1000-7423.2025.01.021
Intestinal protozoa are single-celled eukaryotic microorganisms that are widely distributed in ecological environments. Most of the protozoan isolates are derived from excretions or secretions of individuals carrying cysts, which are used for experimental animal infections following purification and culture. Both the limitations of in vitro culture techniques and the host specificity of protozoa may directly affect the difficulty in the establishment of animal models. This review summarizes major in vitro culture techniques and appropriate animal models, and discusses the factors affecting the establishment of animal models. Considering that novel animal models are more effective to simulate the disease progression of human intestinal protozoan diseases, high attention should be paid to the development of experimental animal models of intestinal protozoan infections, notably novel animal models, so as to provide supports for the establishment and research of animal models of intestinal protozoan infections.
Key words： Intestinal protozoa; In vitro culture; Inoculation; Animal infection; Animal model
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