收稿日期: 2023-06-02
修回日期: 2023-09-12
网络出版日期: 2023-11-06
基金资助
内蒙古自治区自然科学基金(2021MS08063);内蒙古医科大学“科技百万工程”项目(YKD2020KJBW(LH)052);内蒙古自治区科技创新引导项目
Research progress on the structure and function of tick-derived serine protease inhibitor containing Kunitz domains
Received date: 2023-06-02
Revised date: 2023-09-12
Online published: 2023-11-06
Supported by
Natural Science Foundation of Inner Mongolia Autonomous Region(2021MS08063);Inner Mongolia Medical University “Science and Technology Million Project”(YKD2020KJBW(LH)052);Inner Mongolia Autonomous Region Science and Technology Innovation Guidance Project
蜱是一类体外吸血寄生虫，在吸血过程中蜱会向宿主体内释放多种抗凝血物质以保证血液的供应。其中含Kunitz结构域的丝氨酸蛋白酶抑制剂在抗凝血作用中占主要地位。不同的Kunitz型丝氨酸蛋白酶抑制剂具有相似的保守序列，但总体结构不同，在凝血级联中通过抑制丝氨酸蛋白酶类的凝血因子而阻断下游凝血因子的活化，以达到抑制凝血的效果。蜱源Kunitz型丝氨酸蛋白酶抑制剂在凝血系统中的靶点繁多且抑制机制各异。本文对该类蛋白酶抑制剂的抗凝靶点及作用机制进行归纳，有助于抗凝和抗血栓药物的研发，同时进一步解释蜱与宿主间的相互作用以及蜱源抗凝药物的药理作用，为预防蜱对宿主的侵害提供参考资料。
关键词： 蜱; Kunitz型丝氨酸蛋白酶抑制剂; 抗凝血
刘岳青 , 马林源 , 陈开廷 , 高金亮 , 王鹏 . 蜱源Kunitz型丝氨酸蛋白酶抑制分子的结构与功能研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2023 , 41(5) : 625 -630 . DOI: 10.12140/j.issn.1000-7423.2023.05.015
Ticks are blood-sucking ectoparasites. During their blood-sucking process, ticks release variou anticoagulant substances to the hosts to facilitate blood meal. Serine protease inhibitors containing Kunitz domains play an important role in anticoagulation. Different Kunitz serine protease inhibitors have similar conserved sequences but with different overall structures. In the coagulation cascade, inhibition of one or more serine proteases can block the activation of downstream coagulation factors, which could achieve the effect of inhibiting coagulation. Targets for different tick-derived Kunitz serine protease inhibitors in the blood clotting system are varied, and so do their inhibitory mechanisms. To summarize, the anticoagulant targets and anticoagulation mechanisms of those protease inhibitors could contribute to the research and development of anticoagulant and antithrombotic drugs. They could further explain the interaction between ticks and their hosts and the pharmacological action of tick-derived anticoagulant drugs. This can also prevent the invasion of ticks to their hosts.
Key words： Tick; Kunitz type serine protease inhibitor; Anticoagulant
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