微小按蚊种团mtDNA-COII基因变异的研究

中国寄生虫学与寄生虫病杂志 ›› 2002, Vol. 20 ›› Issue (3): 12-170.

微小按蚊种团mtDNA-COII基因变异的研究

周水森;汤林华;郑香

中国疾病预防控制中心寄生虫病预防控制所;中国疾病预防控制中心寄生虫病预防控制所;中国疾病预防控制中心寄生虫病预防控制所世界卫生组织疟疾、血吸虫病和丝虫病合作中心;上海200025;世界卫生组织疟疾、血吸虫病和丝虫病合作中心;上海200025;世界卫生组织疟疾、血吸虫病和丝虫病合作中心;上海200025

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-06-30 发布日期:2002-06-30

Molecular Variation Based on mtDNA-COII Gene of Members of the Anopheles minimus Group

ZHOU Shui sen;TANG Lin hua;ZHENG Xiang

Institute of Parasitic Diseases;Chinese Center for Disease Control and Prevention ;Shanghai 200025

Received:1900-01-01 Revised:1900-01-01 Online:2002-06-30 Published:2002-06-30

摘要/Abstract

摘要：

　　目的　研究我国微小按蚊种团成员 (微小按蚊A/C、乌头按蚊、瓦容按蚊及杰普尔按蚊 )的线粒体DNA(mtDNA)COII基因的分子变异和种系发生关系。　方法　单蚊提取DNA ;mtDNA COII基因的扩增和测序 ;用最大似然法DNAML构建种系发生树。　结果与结论　我国存在微小按蚊A、C两个亲缘种 ,两者COII基因 1 6个位点存在碱基置换 ,突变频率为 2 .3 % ,变异小于其它种团成员

关键词: 微小按蚊种团, 线粒体DNA, 种系发生

Abstract: 　Objective To investigate intra and inter species molecular variations and phylogeny of the five members of the Minimus Group of Anopheles subgenus Cellia in China: An. aconitus, An.varuna, An.jeyporiensis, An.minimus A and An.minimus C, and to report the DNA sequence divergence among these species at a mitochondrial locus (cytochrome oxidase II). Methods Single mosquito′s legs were digested to extract DNA. COII gene was amplified, sequenced and analysed; and the phylogenetic tree of members of An.minimus group was reconstructed by maximum likelihood method (DNAML). Results and Conclusion The data confirmed the presence of two cryptic species, A and C, within An.minimus complex in the research localities; and two species differed by 2.3% in the COII sequences owing to 16 nucleotide substitutions. There was less variation between the two species than other members of the Minimus Group.

Key words: Anopheles minimus group, mitochondrial DNA, phylogeny

周水森;汤林华;郑香. 微小按蚊种团mtDNA-COII基因变异的研究[J]. 中国寄生虫学与寄生虫病杂志, 2002, 20(3): 12-170.

ZHOUShuisen;TANGLinhua;ZHENGXiang. Molecular Variation Based on mtDNA-COII Gene of Members of the Anopheles minimus Group[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2002, 20(3): 12-170.

[1]	尚婧晔, 张光葭, 喻文杰, 何伟, 廖沙, 李汭芮, 黄燕, 刘阳, 钟波. 多房棘球绦虫遗传多态性研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5): 637-641.
[2]	周成艳, 马君, 刘伟, 徐前明, 朱兴全. 印度华首线虫核糖体和线粒体基因序列多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2019, 37(1): 81-86.
[3]	李雯雯1，2，任怡静2，李健3，黄维义2，4 *. 陶氏颚口线虫成虫的扫描电镜观察及基于ITS2和COX1基因的系统发生分析[J]. 中国寄生虫学与寄生虫病杂志, 2015, 33(2): 13-130-134.
[4]	刘海鹏;曹建平;沈玉娟;陈有贵;李小红;卢潍媛;徐馀信;刘宜升;刘述先;周晓农;汤林华. 隐孢子虫牛源分离株的分离和鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(2): 1-86.
[5]	吕洪刚,胡孝素. 利什曼原虫动基体DNA微环同源性的分析及其克隆[J]. 中国寄生虫学与寄生虫病杂志, 1988, 6(S1): 38-39.