泡球蚴感染BALB/c小鼠IgG亚类和细胞因子的动态观察

中国寄生虫学与寄生虫病杂志 ›› 2003, Vol. 21 ›› Issue (6): 11-360.

泡球蚴感染BALB/c小鼠IgG亚类和细胞因子的动态观察

李富荣,石佑恩,史大中,D.A.Vuitton,P.S.Craig

深圳市人民医院,华中科技大学同济医学院寄生虫学教研室,兰州医学院寄生虫学教研室,法国贝尚松大学中心医院,英国苏尔沃德大学生命科学院暨南大学医学院第二附属医院临床医学研究中心,深圳 518020,武汉 43000,兰州 730000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-12-30 发布日期:2003-12-30

Kinetic Analysis of Cytokines and Immunoglobulin G Subclass in BALB/c Mice Infected with Echinococcus alveolaris

LI Fu-rong~1,SHI You-en~2,SHI Da-zhong~3,DA Vuitton~4,PS Craig~5

1 Clinical Medical Research Center,Shenzhen People’s Hospital,Second Affiliated Teaching Hospital of Ji’nan University,Shenzhen 518020 2 Department of Parasitology,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030 3 Department of Parasitology,Lanzhou Medical College,Lanzhou 730000 4 Centre Hospitalier Universitaire,Besancon,France 5 Departmentof Biological Sciences,University of Salford(UK)

Received:1900-01-01 Revised:1900-01-01 Online:2003-12-30 Published:2003-12-30

摘要/Abstract

摘要： 　　目的　观察小鼠感染泡球蚴后其体液免疫的动态变化。　方法　BALB/c小鼠感染泡球蚴后,分别于2、4、8、12、16、20及25wk(IgG含量达高峰)取脾脏制备淋巴细胞悬液体外培养,分别以多房棘球蚴抗原(EmAg)、伴刀豆球蛋白A(ConA)刺激诱生可溶性白细胞介素2受体(IL2R)、肿瘤坏死因子α(TNFα)及白细胞介素1(IL1);以植物凝集素(PHA)刺激诱生干扰素γ(IFNγ)。检测培养上清中IL2R、TNFα、IL1及IFNγ含量。各组均设RPMI1640培养液平行对照。检测血清中一氧化氮(NO)及特异性免疫球蛋白G(IgG)亚类水平。　结果　小鼠感染泡球蚴16wk后NO水平明显升高,IgG、IgG1和IgG3水平升高,IgG2a及IgG2b呈低水平。感染后的前12wk,脾淋巴细胞以分泌IL2R和TNFα为主,12wk后以IFNγ为主、16wk后以IL1为主。　结论　小鼠感染泡球蚴后的前8wk呈Th1反应。感染后期Th2反应逐渐增强

关键词: 泡球蚴, BALB/c小鼠, 细胞因子, 免疫球蛋白G亚类, 一氧化氮

Abstract: 　Objective To observe the dynamic change of immune response in mice infected with Echinococcus alveolaris(AE) at difference period of time, and to explore hostalveo’s immune regulation. Methods The infection lasted and was followed up for 25 weeks. The spleen cells from BALB/c mice infected with AE stimulated with EmAg and ConA or PHA in vitro . IL-2R, IFN-γ, TNF-α, IL-1 and specific IgG subclasses were determined by ELISA. NO was tested by chemical assay. Results NO level sharply rised in 16 weeks after BALB/c mice were infected with AE. The levels of IgG1 and IgG3 significantly increased 8 weeks after infection, and remained elevating throughout the period of observation. IgG3 showed slight increase, IgG2a and IgG2b appeared low level following infection. The production of IL-2R and TNFα increased significantly 8 weeks of infection, while IL-2R sharply decreased in 12 weeks of infection. During the period of 2-12 weeks of infection there was an increase in IL-1 secreting. The level of IL-1 and TNFα rapidly increased since 16 weeks post infection. High level of IFN-γ was detected during the period of observation, and showed a peak at 12 weeks. Conclusion Th1 is the major response in the early stage of infection,which is replaced by Th2 response in later period of infection.

Key words: Echinococcus alveolaris, BALB/c mice, cytokine, IgG subclass, NO

李富荣;石佑恩;史大中;D.A.Vuitton;P.S.Craig. 泡球蚴感染BALB/c小鼠IgG亚类和细胞因子的动态观察[J]. 中国寄生虫学与寄生虫病杂志, 2003, 21(6): 11-360.

LIFu-rong~;SHIYou-en~;SHIDa-zhong~;DAVuitton~;PSCraig~. Kinetic Analysis of Cytokines and Immunoglobulin G Subclass in BALB/c Mice Infected with Echinococcus alveolaris[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2003, 21(6): 11-360.

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