中国寄生虫学与寄生虫病杂志 ›› 1984, Vol. 2 ›› Issue (4): 228-231.

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淡色库蚊卵巢滞育指标及其内分泌控制滞育的观察

王仁赉,张恩英,钱宗立   

  1. 上海第二医学院寄生虫学教研室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1984-11-30 发布日期:2017-01-12

OBSERVATIONS ON CRITERIA OF OVARIAN DIAPAUSE IN CVLEX PIPIENS PALLENS AND ITS HORMONE CONTROL

  • Received:2017-01-12 Revised:2017-01-12 Online:1984-11-30 Published:2017-01-12

摘要: 通过淡色库蚊卵泡发育和滞育相对照,观察到滞育指标为第一卵泡停滞在羽化后24个时、长约50μm的N期,同时可用原卵区(第二卵泡加第三卵泡)和第一卵泡几乎等长作为滞育的佐证。进入Ⅰ_a期开始均属发育蚊。Ⅰ_a期是卵泡早期发育的最旺盛时期,并开始吸血,在不供血情况下,可继续发育直至停留在Ⅱ_b休止期。 通过蚊虫去首替代脑抑制咽侧体分泌保幼激素的方法,与滞育蚊对照。推论卵泡滞育与缺乏保幼激素有关;第一卵泡从羽化时的No.1期发育增至N期,似乎与保幼激素无关,但从N期进一步发育则依赖咽侧体分泌保幼激素的作用,咽侧体释放保幼激素的临界时间约在雌蚊羽化后24小时左右。

关键词: 保幼激素, 淡色库, 内分泌控制, 咽侧体, 卵泡发育, 原卵区, 短日照, 尖音库蚊, 滋卵细胞, 蚊卵

Abstract: By comparative studies of the follicle development and mosquito diapause, the major criterion of diapause in adult C, pipiens pallens has been demonstrated to be the retardation of the first follicle at N-stage, which occurred 24 hours after emergence, with a length around 50 micrometers. The almost equal length of the first follicle and the germarium (i.e. second follicle plus third follicle) may also be considered as a secondary evidence of the diapause. Developing mosquitoes are indicated by the appearance of la stage which is the most prosperous period of the follicle development in its early stage when the mosquitoes start blood-feeding. In the absence of blood supply; development may continue up to and then stop at the resting stage of IIb.The process of follicle development in diapaused adults was carefully compared with that of decapitated individuals which were taken as an alternative of brain inhibition to juvenile hormone (JH) secretions of the corpora allata (CA). Results strongly inferred that follicle retardation may be attributed to lack of JH. It is suggested that the develpment of the first follicle from stage No.1 at emergence to N-stage may be unrelated to JH, while further development from N-stage may depend on the function of JH from CA. The critical point of JH secretion from CA was demonstrated at around 24 hours after emergence of the female adult.