关键词: 肝片吸虫, 分泌抗原, 基因文库, 免疫印迹

Abstract: This paper reported the immunoscreening of three recombinants expressing secretory antigens from the genomic DNA library of F. hepatica and the primary study of expression of the three recombinants. To construct the genomic DNA library, DNA from the adult F. hep-atica was cut with Sau3AI to an average length of about 2. 0 kb and inserted into the BamHI site of the expression vector pUC18, the size of the genomic DNA library of F. hepatica being 1. 5×105 recombinants.Three recombinants expressing antigenic determinants of F. hepatica pFHl6, pFH23, pFH48 were detected through primary screening and rescreenging with F. hepatica infected rabbit serum(1 : 50) preabsorbed to remove antibodies to E. colt and SPA-HRP(1 : 40). The test of the ability of expressing fusion proteins showed pFH16pFH48pFH23. These studies provide the possibility of further research on the expression of recombinant antigenic genes, the immunity of their expressed products and the protection of animals.