中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (2): 16-106.

• 论著 • 上一篇    下一篇

日本血吸虫大陆株副肌球蛋白部分基因克隆

邱持平,童小妹,刘述先   

  1. 中国预防医学科学院寄生虫病研究所  上海 200025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-04-30 发布日期:1997-04-30

CLONING OF A PARTIAL PARAMYOSIN GENE FROM SCHISTOSOMA JAPONICUM

Qiu Chiping, Tong Xiaomei, Liu Shuxian   

  1. Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-04-30 Published:1997-04-30

摘要:

目的: 克隆日本血吸虫大陆株副肌球蛋白部分基因。方法: 用PCR方法根据已发表日本血吸虫菲律宾株副肌球蛋白基因部分核苷酸序列扩增大陆株副肌球蛋白基因。结果: 获得一日本血吸虫大陆株720 bp基因克隆。结论: 经核苷酸序列分析证实, 本克隆基因与菲律宾株基因核苷酸同源性为99.03%。

关键词: 日本血吸虫, 副肌球蛋白, 基因克隆, 疫苗, PCR, 核苷酸序列

Abstract:

AIM: To clone apartial paramyosin gene from Schistosoma japonicum of Chinese mainland origin. METHODS: The gene was amplified by PCR according to the published partial paramyosin sequence of S. japonicum of Philippine origin. RESULTS: A gene of about 720bp was cloned from S. japonicum of Chinese mainland origin. CONCLUSION: The analysis of the sequence of this gene revealed 99.03% identity with that of S. japonicum of Philippine origin.

Key words: Schistosoma japonicum, paramyosin, gene cloning, PCR, vaccine, sequence