中国寄生虫学与寄生虫病杂志

• 研究简报 • 上一篇    下一篇

琼脂-石蜡双包埋法用于湖北钉螺软体组织结构的观察

谭苹*,张洁,李青,于志君   

  1. 武汉科技大学医学院寄生虫学教研室,武汉 430065
  • 出版日期:2014-12-30 发布日期:2015-02-12

An Observation on the Histological Structure of Oncomelania hupensis Soft Tissue by Agar-Paraffin Double-embedding Method

TAN Ping*,ZHANG Jie,LI Qing,YU Zhi-jun   

  1. Department of Parasitology,Medical College of Wuhan Science and Technical University,Wuhan 430065,China
  • Online:2014-12-30 Published:2015-02-12

摘要:

为观察和研究湖北钉螺软体的组织学结构,将固定后的湖北钉螺软体组织包埋于琼脂中,然后完成脱水、透明、浸蜡、切片和染色等步骤,得到湖北钉螺软体组织的永久玻片标本,光镜下可见清晰的软体组织结构。

关键词: 湖北钉螺, 软体组织, 琼脂-石蜡双包埋

Abstract:

In order to study the histological structure of Oncomelania hupensis soft tissue, the fixed soft tissues of O. hupensis were pre-embedded in the agar and made blocks, then dehydrated, transparentized, immersed in paraffin, sectioned, and stained with haematoxylin-eosin(HE). Permanent slides of O. hupensis soft tissue were obtained. The histological structure of soft tissues was clear under the microscope.

Key words: Oncomelania hupensis, Soft tissue, Agar-Paraffin double-embedded