关键词:  , 细粒棘球蚴；亲肌肉抗原重组蛋白；脾细胞；免疫保护

Abstract: 【Abstract】   Objective   To investigate the immune response and the protection in mice induced by the recombi-nant myophilin protein of Echinococcus granulosus.  Methods  Thirty-six male ICR mice of 6-8 weeks old were randomly divided into groups A, B and C each with 12. The mice in the 3 groups were subcutaneously immunized with Eg myophilin protein, blank plasmid protein or PBS, respectively, by 3 times and challenged with protoscoleces of E. granulo-sus 2wk after the last vaccination. Mice were sacrificed 20wk after the infection, the hydatid cysts were collected for mea-suring the weight reduction. Spleens were obtained and the splenocytes were separated and cultured in vitro with EgAg or ConA stimulus for 4-5 h. The subsets of CD4+ and CD8+ T cells were measured by FACsort. The proliferation of spleno-cytes was determined by MTT method with blank plasmid and PBS as control.  Results  The average weight of the hy-datid cysts in the immunized group decreased by 69.1% in comparison to the blank plasmid and PBS groups. The CD4+ subset [(29.7±0.9)%] and CD8+ subset [（9.7±0.8）%] in group A increased significantly than group C, [(11.6±1.4)%] and [(7.8±0.2)%] respectively (P<0.01 or <0.05). The ratio of CD4+/CD8+ subsets in group A (3.061±0.015) was also higher than group C (1.487±0.106) (P<0.01). Without stimulation, the proliferation of T lymphocytes in group A（0.237±0.009）was high-er than group C (0.159±0.005) (P<0.01)， with EgAg or ConA stimulus, it was also higher in group A than that of group C(P<0.01).  Conclusion  The recombinant myophilin protein of E. granulosus can induce the proliferation of splenocytes and Th1 response in mice, and the CD4+ T cells subset may bear a part in the induced protection against the challenge of protoscoleces.