中国寄生虫学与寄生虫病杂志 ›› 2012, Vol. 30 ›› Issue (5): 18-372-373.

• 研究简报 • 上一篇    下一篇

贵州省黑斑蛙体内裂头蚴扫描电镜观察

徐婧1,陈艳1 *,唐贵文1,刘鲜林2   

  1. 1 贵阳医学院人体寄生虫学教研室,贵阳550004;
    2 贵阳医学院科研处电镜室,贵阳550004
  • 出版日期:2012-10-30 发布日期:2013-02-05

Scanning Electron Microscope Observation of Plerocercoid from  Rana nigromaculata in Guizhou Province

XU Jing1,CHEN Yan1 *,TANG Gui-wen1,LIU Xian-lin2   

  1. 1 Department of Parasitology; 2 Section of Electron Microscopy, Guiyang Medical College,Guiyang 550004, China
  • Online:2012-10-30 Published:2013-02-05

摘要:  【提要】  自野生黑斑蛙体内检获的裂头蚴,选择5条完整、活动度好的虫体,用戊二醛?鄄锇酸双重固定,经乙醇梯度脱水、真空冷冻干燥、喷金后,用扫描电镜观察并拍照。结果发现,虫体前后两端略膨大,不分节,但表面有不规则环形皱褶;体前端无吸槽,顶端中央有一横向凹陷,凹陷周围体壁呈唇状突起;体后端形态类似前端,但中央为一裂隙,裂隙较前端的凹陷浅、窄;体表的质膜内陷形成大小不等的凹窝和沟纹;整个虫体体表密布尖棘状长短不一的微毛。

关键词: 黑斑蛙, 裂头蚴, 扫描电镜

Abstract: 【Abstract】  Five integral and active plerocercoids collected from naturally infected frogs were fixed by glutaraldehyde?-osmic acid, dehydrated in graded series of ethanol, dried via vacuum freeze and coated with carbon gold. They were then observed by scanning electron microscopy. The results showed that the anterior and posterior ends of the plerocercoids were slightly swell and without segmentation. Annular furrows ran irregularly along the body surface. The anterior end was drawn inside and had a deep dorsoventral hollow, around which, the plasma membrane displayed a lip bulge. The posterior end was similar to the anterior one in the shape, but a fissure in its central part was comparatively narrower and shallower than the hollow in the anterior end. There were thousands of pits and grooves on the body surface, unequal in size. The whole body surface of the plerocercoid was densely covered by sharp spine-like microtriches.

Key words: Rana nigromaculata, Plerocercoid, Scanning electron microscopy