扩展莫尼茨绦虫不同体节翻译延伸因子和引发酶mRNA转录水平研究

中国寄生虫学与寄生虫病杂志 ›› 2012, Vol. 30 ›› Issue (3): 21-250-252.

扩展莫尼茨绦虫不同体节翻译延伸因子和引发酶mRNA转录水平研究

王正荣，薄新文*，赵文娟，康立超

1 新疆农垦科学院，石河子 832000； 2 新疆绵羊重点繁育实验室，石河子 832000

出版日期:2012-06-30 发布日期:2012-09-28

mRNA Transcription of Translation Elongation Factor and Primase in Different Segments of Moniezia expansa

WANG Zheng-rong, BO Xin-wen*, ZHAO Wen-juan, KANG Li-chao

1 Xinjiang Academy of Agricultural and Reclamation Science，Shihezi 832000，China； 2 Key Labaratory of Sheep Breed and Biotechnology，Shihezi 832000，China

Online:2012-06-30 Published:2012-09-28

摘要/Abstract

摘要： 本研究以β微管蛋白基因作为内参基因，采用实时荧光定量PCR方法检测该基因在扩展莫尼茨绦虫（Moniezia expansa）4个不同发育阶段，即头节、幼节、成节和孕节中的表达差异。标准曲线分析显示，翻译延伸因子、引发酶基因和β微管蛋白基因标准质粒实时定量扩增Ct值与标准质粒的浓度均呈良好的线性关系，相关系数（R²）均大于0.99。同时实验结果显示，翻译延伸因子和引发酶基因在虫体各发育阶段中的转录水平存在差异。其中，翻译延伸因子和引发酶基因在扩展莫尼茨绦虫头节和幼节的转录水平均较高，提示这2个基因可能在头节和幼节的发育中有重要作用。

关键词: 扩展莫尼茨绦虫, 翻译延伸因子, 引发酶, mRNA转录水平

Abstract: A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship (>0.99) between the Ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. Real-time PCR showed that the expression abundance of translation elongation factor and primase was different. In conclusion, the transcription level of translation elongation factor and primase was high in both scolex and immature segment, suggesting that they may play a role in the development of scolex and immature segment.

Key words: Moniezia expansa, Translation elongation factor, Primase, mRNA transcription

王正荣，薄新文*，赵文娟，康立超. 扩展莫尼茨绦虫不同体节翻译延伸因子和引发酶mRNA转录水平研究[J]. 中国寄生虫学与寄生虫病杂志, 2012, 30(3): 21-250-252.

WANG Zheng-rong, BO Xin-wen*, ZHAO Wen-juan, KANG Li-chao. mRNA Transcription of Translation Elongation Factor and Primase in Different Segments of Moniezia expansa[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2012, 30(3): 21-250-252.