中国寄生虫学与寄生虫病杂志 ›› 2010, Vol. 28 ›› Issue (1): 3-16.

• 论著 • 上一篇    下一篇

广东省广州管圆线虫病疫源地调查

邓卓晖1 *, 张启明1, 林荣幸1, 黄少玉1, 张仪2, 吕山2
刘和香2, 胡铃2, 裴福全1, 王金龙1, 阮彩文1
  

  1. 1 广东省疾病预防控制中心寄生虫病防治研究所,广州 510300;2 中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海 200025
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-28 发布日期:2010-02-28

Survey on the Focus of Angiostrongylus cantonensisin Guangdong Province

DENG Zhuo-hui1 *,ZHANG Qi-ming1,LIN Rong-xing1,HUANG Shao-yu1,ZHANG Yi2,LV Shan2,LIU He-xiang2,HU Ling2,PEI Fu-quan1,WANG Jin-long1,RUAN Cai-wen1   

  1. 1 Center for Disease Control and Prevention of Guangdong Province,Guangzhou 510300,China;2 National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention;WHO Collaborating Centre for Malaria,Schistosomiasis and Filariasis,Shanghai 200025,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-28 Published:2010-02-28

摘要: 目的 了解广东省广州管圆线虫病疫源地分布情况,为制定监测方案提供依据。 方法 按不同地理位置分层抽样,分别抽取粤东、粤西、粤北山区和珠江三角洲4个地区共22个县(市、区),每县(市、区)随机抽取1~2个行政村(区)作为调查点。用匀浆法或肺检法剖检在野外现场采集的部分小管福寿螺(Pomacea canaliculata)和褐云玛瑙螺(Achatina fulica),以及餐饮、集市采集的不同品种的淡水螺和陆生螺,调查广州管圆线虫(Angiostrongylus cantonen?鄄sis)Ⅲ期幼虫感染情况。现场捕捉鼠类,鉴别种类后解剖心肺组织,检查感染情况;采集新鲜鼠粪,用清水沉淀法检查幼虫。 结果 22个调查县(市、区)均有小管福寿螺分布,且数量众多;广宁县未开展褐云玛瑙螺调查,和平县、曲江区和翁源县未采集到褐云玛瑙螺,其他调查点均有该螺分布。22个调查县(市、区)共采集小管福寿螺3 754只,褐云玛瑙螺1 465只,平均感染率分别为5.9%(172/2 929)和16.5%(223/1 354),两者差异有统计学意义(P<0.01)。粤东、粤西、粤北山区和珠江三角洲的受检螺平均感染率有地区差异(P<0.01),珠江三角洲最高(15.6%,152/975),其中又以东莞市的受检螺平均感染率最高(34.7%,78/225)。在9个调查县(市、区)的农贸市场采集圆田螺(Cipangopaludina)114只和环棱螺(Bellamya)541只,罗定市和开平市的环棱螺中幼虫阳性率分别为1.4%(1/70)和3.3%(3/91)。在9个调查县(市、区)捕获褐家鼠(Rattus norvegicus)、黄胸鼠(R. flavipectus)、鼩鼱(Suncus murinus)、小家鼠(Mus mus?鄄culus)、大板齿鼠(Bandicota indica)、黄毛鼠(R. losea)和黑家鼠(R. rattus)等共491只,平均感染率为11.4%(56/491),其中褐家鼠、黄胸鼠和板齿鼠的平均感染率分别为19.8%(52/263),2.5%(3/118)和10.0%(1/10),其他4种鼠类中均未发现有广州管圆线虫感染。在7个调查县(市、区)采集到新鲜鼠粪34份,Ⅰ期幼虫阳性率为44.1%(15/34)。 结论 广东省广州管圆线虫病疫源地分布广泛,中间宿主和终末宿主均有不同程度感染。

关键词: 广州管圆线虫, 小管福寿螺, 褐云玛瑙螺, 宿主, 疫源地, 广东

Abstract: Objective To understand the distribution of Angiostrongylus cantonensis foci in Guangdong Province for making surveillance program. Methods Survey sites were chosen by strata sampling according to different geographic locations. Totally 22 survey sites were selected in four regions: East Guangdong, West Guangdong, North Guangdong and the Pearl River Delta. One or two administrative villages in each site were randomly selected for the investigation. Pomacea canaliculata and Achatina fulica collected from fields and other species of freshwater or terrestrial snails obtained in the restaurants and wet markets were examined for the third stage larvae by tissue grinding or lung examination. Rats were captured in the fields, and their hearts and lungs were dissected for adult worms. Rat feces were also collected for the detection of first stage larvae by water precipitation. Results Large number of P. canaliculata was found in all sites. A. fulica was found in most surveyed sites. Totally 2 929 P. canaliculata and 1 354 A. fulica were collected with a larva infection rate of 5.9% (172/2 929) and 16.5% (223/1 354), respectively (P<0.01). The average prevalence among the regionswas different (P<0.01) with the highest prevalence in Pearl River Delta (15.6%, 152/975), especially in Dongguan City of the Delta (34.7%, 78/225). 114 Cipangopaludina sp. and 252 Bellamya sp. were bought from wet markets of 9 sites. Larvae were found only in Bellamya snails from Luoding and Kaiping cities with an infection rate of 1.4% (1/70) and 3.3% (3/91), respectively. Totally 491 rats were captured in 9 sites including Rattus norvegicus, R. flavipectus, Suncus murinus, Mus musculus, Bandicota indica, R. losea and R. rattus, with an average infection rate of 11.4% (56/491). Adult worms were found in R. norvegicus, R. flavipectus and B. indica with a prevalence of 19.8% (52/263), 2.5% (3/118) and 10.0% (1/10), respectively. Thirty-four rodent fecal samples were collected in 7 sites and examined with a larva positive rate of 44.1% (15/34). Conclusion Foci of Angiostrongylus cantonensis are widely distributed in Guangdong Province as natural infection has been found in its intermediate and definitive hosts.

Key words: Angiostrongylus cantonensis, Pomacea canaliculata, Achatina fulica, Host, Focus, Guangdong