银杏酸5种同系物单体的制备及其杀灭钉螺的作用

中国寄生虫学与寄生虫病杂志 ›› 2007, Vol. 25 ›› Issue (4): 2-278.

银杏酸5种同系物单体的制备及其杀灭钉螺的作用

毛佐华1;俞培忠2; 孙锴3;潘小海4; 姜庆五5;潘家祜3

1 复旦大学上海医学院病原生物学系, 上海 200032; 2 复旦大学药学院天然药物化学教研室, 上海 200032; 3 复旦大学药学院药理学教研室, 上海 200032; 4 复旦大学公共卫生学院劳动卫生教研室, 上海 200032; 5 复旦大学公共卫生学院流行病学教研室, 上海 200032

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-30 发布日期:2007-08-30
通讯作者: 潘家祜

Preparation of Five Ginkgolic Acid Monomers and Their Molluscicidal Effects Against Oncomelania hupensis

MAO Zuo-hua1; YU Pei-zhong2;SUN Kai3; PAN Xiao-hai4;JIANG Qing-wu5;PAN Jia-hu3

1 Department of Parasitology and Microbiology, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2 Department of Chemistry of Natural Drugs, School of Pharmacy, Fudan University, Shanghai 200032, China; 3 Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 200032, China; 4 Department of Occupational Health, School of Public Health, Fudan University, Shanghai 200032, China; 5 Department of Epidemiology, School of Public Health, Fudan University, Shanghai 200032, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-30 Published:2007-08-30
Contact: PAN Jia-hu

摘要/Abstract

摘要： 【摘要】目的探索分离纯化的银杏酸同系物单体对钉螺的影响。方法用石油醚从银杏外种皮提取银杏酸, 经半制备高效液相色谱（HPLC）分离, 通过液相?鄄质谱联机系统鉴定。按WHO“杀螺剂实验室终筛方法”检测银杏酸分离纯化的5个同系物单体杀灭湖北钉螺的作用。结果纯化的银杏酸5个同系物单体为GA _13︰0 、 GA _15︰1 、 GA _15︰0 、 GA_17︰1 和GA_17︰2 , 其母核苯环上分别有1个13、 15及17烷基或烯基的侧链。各单体占总银杏酸的比例分别为17.6%、 52.3%、 3.2%、 23.3% 和3.6%。杀螺活性顺序为： GA _13︰0>GA_15︰1>GA _15︰0>GA _17︰1>GA _17︰2 。 24 h对钉螺的半数致死浓度（LC₅₀）依次为20.79、 22.28、 33.76、 51.89和59.10 mg/L。GA _13︰0 和 GA_15︰1对钉螺上爬的抑制作用明显。结论银杏酸单体杀灭钉螺的作用受其侧链碳原子数量以及所含双键数目的影响, 单体GA _13︰0及GA _15︰1杀钉螺作用明显, 并能显著抑制钉螺上爬。单体GA15︰0也有一定的杀螺活性。

关键词: 银杏酸, 灭螺剂, 钉螺, 单体

Abstract: 【Abstract】 Objective To investigate the molluscicidal activities of the ginkgolic acid(GA) monomers isolated and purified from GAs. Methods Five monomers of GAs from the sarcotesta of Ginkgo biloba. were extracted by petrol ether, separated by silica gel column chromatography, purified by semi-prepared reversed-phased HPLC, and identified by LC-MS analysis. The molluscicidal activities of GAs and their monomers against Oncomelania hupensis were determined as referring to the WHO guidelines for laboratory molluscicidal test. Results The five purified ginkgolic acid monomers were GA_13︰0, GA _15︰0, GA _15︰1 , GA _17︰1 and GA _17︰2 , with a side chain of 13, 15, 17 alkyl or ethylenic radicals respectively on their benzene loop. The five monomer proportions to the total GAs were 17.6%, 3.2%, 52.3%, 23.3% and 3.6% respectively. The order of molluscicidal activities for the five monomers was as follows: GA _13︰0>GA _15︰1>GA _15︰0>GA _17︰1>GA _17︰2, and their LC50 for snails was 20.79 mg/L, 22.28 mg/L, 33.76 mg/L, 51.89 mg/L, and 59.10 mg/L respectively after immersion for 24 hours. Two monomers, GA _13︰0 and GA15︰1 inhibited the snails' climbing up significantly. Conclusion The molluscicidal activities of GAs may be dependent on the monomer's structure with different number of carbon molecules and double-bonds on the side carbon-chain. The two monomers, GA _13︰0 and GA _15︰1 , are mainly responsible for the molluscicidal activities of GAs and both effectively inhibit snails' climbing up as well. GA _15︰0 also shows certain molluscicidal activity.

Key words: Ginkgolic acid, Molluscicide, Oncomelania hupensis, Monomer

毛佐华;俞培忠;孙锴;潘小海;姜庆五;潘家祜. 银杏酸5种同系物单体的制备及其杀灭钉螺的作用[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(4): 2-278.

MAOZuo-hua;YUPei-zhong;SUNKai;PANXiao-hai;JIANGQing-wu;PANJia-hu. Preparation of Five Ginkgolic Acid Monomers and Their Molluscicidal Effects Against Oncomelania hupensis[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2007, 25(4): 2-278.

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