利用MGE-PCR技术研究锥虫亚属种间与亚种间的分子特性

中国寄生虫学与寄生虫病杂志 ›› 2005, Vol. 23 ›› Issue (5): 6-282.

利用MGE-PCR技术研究锥虫亚属种间与亚种间的分子特性

李凤军,郑嘉瑜,贾万忠,伦照荣

中山大学生命科学学院，华南寄生生物研究中心，广州 510275

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-10-30 发布日期:2005-10-30

Analysis of Molecular Profiles Among Trypanozoon Speciesand Subspecies By MGE-PCR Method

LI Feng-jun,ZHENG Jia-yu,JIA Wan-zhong,LUN Zhao-rong*

Center for Parasitic Organisms, School of Life Sciences, Zhongshan (Sun Yat-Sen ) University, Guangzhou 510275, China

Received:1900-01-01 Revised:1900-01-01 Online:2005-10-30 Published:2005-10-30

摘要/Abstract

摘要： 目的分析锥虫亚属布氏锥虫、伊氏锥虫和马媾疫锥虫3个种以及布氏锥虫：指名亚种、冈比亚亚种、罗得西亚3个亚种间的遗传变异与进化关系。方法利用移动遗传因子（MGE）-PCR技术对26个锥虫虫株基因组进行扩增，同时利用邻位相连（NJ）法对扩增产物进行聚类分析。结果锥虫亚属的种间及亚种间的遗传差异明显，遗传距离在0到100% 间均有分布（平均遗传距离为41.2%）；布氏锥虫种内遗传变异度最大，遗传相似系数只有41.15%，伊氏锥虫和布氏锥虫的同源性最高，相似系数为62.94%；布氏锥虫指名亚种和罗得西亚亚种明显高于冈比亚亚种的遗传变异程度。结论锥虫亚属的种及亚种间的遗传变异程度较高；中国与南美洲的伊氏锥虫及中国与南美的马媾疫锥虫均应为同一起源。

关键词: 锥虫亚属, 伊氏锥虫, 马媾疫锥虫, 布氏锥虫指名亚种, 布氏锥虫罗得西亚亚种, 布氏锥虫冈比亚亚种, 移动遗传因子-PCR, 聚类分析

Abstract: Objective To analyze the relationship between genetic variability and evolution among Trypanosoma brucei (including T. b. brucei, T. b. rhodesiense and T. b. gambiense), T. evansi and T. equiperdum isolates. Methods Genomic DNAs of 26 trypanosome isolates were amplified by a mobile genetic elements (MGE)-PCR technique and cluster analysis was performed based on the molecular profiles with Neighbor-Joining method. Results The genetic variability among trypanosome isolates examined was obvious with an average genetic distance of 41.2% (ranged from 0 to 100%). Similarity coefficient among T. brucei isolates was 41.15% which was lower than that between T. evansi and T. equiperdum isolates. The closest relationship was found between T. evansi and T. brucei isolates with a similarity coefficient of 62.94%. The genetic variability between T. b. rhodesiense and T. b. brucei isolates was higher than that among T. b. gambiense isolates. Conclusion Species and subspecies in Trypanozoon displayed a higher genetic variability; T. equiperdum isolates collected from China and from South America, and T. evansi isolates from China and from South America, should have a similar origin.

Key words: Trypanozoon, Trypanosoma evansi, T. equiperdum, T. b. brucei, T. b. rhodesiense, T. b. gambiense, MGE-PCR, Cluster analysis

李凤军;郑嘉瑜;贾万忠;伦照荣. 利用MGE-PCR技术研究锥虫亚属种间与亚种间的分子特性[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(5): 6-282.

LIFeng-jun;ZHENGJia-yu;JIAWan-zhong;LUNZhao-rong*. Analysis of Molecular Profiles Among Trypanozoon Speciesand Subspecies By MGE-PCR Method[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(5): 6-282.

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