Ⅱ类人白细胞抗原等位基因与晚期肝脾型日本血吸虫病的相关性研究

中国寄生虫学与寄生虫病杂志 ›› 2005, Vol. 23 ›› Issue (1): 2-9.

Ⅱ类人白细胞抗原等位基因与晚期肝脾型日本血吸虫病的相关性研究

章俊华,刘文琪,李雍龙*,龙小纯

华中科技大学同济医学院寄生虫学教研室, 武汉 430030

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-02-28 发布日期:2005-02-28

Studies on the Association of Human Leukocyte Antigen Class Ⅱ Alleles with Advanced Hepatosplenic Schistosomiasis japonica

ZHANG Jun-hua;LIU Wen-qi;LI Yong-long *;LONG Xiao-chun

Department of Parasitology，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China

Received:1900-01-01 Revised:1900-01-01 Online:2005-02-28 Published:2005-02-28

摘要/Abstract

摘要： 目的　探讨Ⅱ类人白细胞抗原 (HLA-Ⅱ)基因多态性与晚期肝脾型日本血吸虫病的相关性。　方法　用聚合酶链反应-序列特异性引物 (PCR-SSP)技术对 46例晚期肝脾型日本血吸虫病患者 (实验组 )和 43例慢性日本血吸虫病患者 (对照组 )HLA-DRB1、DPA1、DQA1和DQB14个基因位点的等位基因进行分型。对两组间等位基因频率的差异进行 χ² 检验。　结果　实验组HLA-DRB1*04、DPA1*0103、DQA1*0601和DQB1*020 1等位基因频率明显高于对照组 ,而HLA-DQA1*0501和DQB1*0601等位基因频率明显低于对照组。　结论　HLA-DRB1*04、DPA1*0103、DQA1*0601和DQB1*0201等位基因 ,因其与晚期肝脾型日本血吸虫病呈显著的正相关 (P <0.0 5 )而可能是该病的遗传易感基因 ,而HLA-DQA1*0501和DQB1*0601等位基因与对该病存在抵抗性有关。

关键词: 肝脾型血吸虫病, HLA-Ⅱ抗原, 等位基因

Abstract: Objective To study the association of human leukocyte antigen classⅡ(HLA-Ⅱ) alleles with genetic susceptibility and resistance to advanced hepatosplenic schistosomiasis japonica. Methods The allelic types of HLA-DRB1, DPA1, DQA1 and DQB1 were detected by polymerase chain reaction with sequence-specific primers (PCR-SSP) technique in 46 patients with advanced hepatosplenic schistosomiasis, characterized with extensive liver fibrosis. Another 43 subjects with chronic schistosomiasis were used as control. The statistical significance of differences in allelic frequencies was determined by χ ²test. Results The frequencies of HLA-DRB1*04, DPA1*0103, DQA1*0601, DQB1*0201 in advanced patients were markedly higher than those in control group, while the frequencies of HLA-DQA1*0501 and DQB1*0601 in control group were higher than those in advanced patients. Conclusion The study indicated that HLA-DRB1*04, DPA1*0103, DQA1*0601 and DQB1*0201 showing a positive, statistically significant (P<0.05) association with advanced hepatosplenic schistosomiasis japonica may be the susceptible genes, whereas HLA-DQA1*0501 and DQB1*0601 may be more relevant to a resistance to the disease.

Key words: Hepatosplenic schistosomiasis, HLA-Ⅱ antigens, Alleles

章俊华;刘文琪;李雍龙;龙小纯. Ⅱ类人白细胞抗原等位基因与晚期肝脾型日本血吸虫病的相关性研究[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(1): 2-9.

ZHANGJun-hua;LIUWen-qi;LIYong-long*;LONGXiao-chun. Studies on the Association of Human Leukocyte Antigen Class Ⅱ Alleles with Advanced Hepatosplenic Schistosomiasis japonica[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2005, 23(1): 2-9.

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