关键词: 猪囊尾蚴, 循环抗原, 单克隆抗体, 双特异单克隆抗体

Abstract:

AIM:To detect serum circulating antigen in cysticercosis cases using monoclonal antibodies. METHOD:Four hybridoma cell lines,two secreting anti Cysticercus mono specific monoclonal antibodies(McAb)and two secreting anti SRBC and anti Cysticercus bi specific McAbs were raised and coded as Ⅱ03,I 06D5, Ⅱ03G2 and Ⅱ03F4,respectively. RESULTS:These McAbs were used to set up reversed IHA(RIHA) assays for circulating antigen(CAg) detections in cysticercosis. Sera from four groups of documented patients,viz.,120 pretreated,32 treated with praziquantel 20 mg·kg -1 ·d -1 for 9 d for 4 courses,23 treated with 7 courses and 15 from taeniasis,were tested,giving positive rates of 90%,37.5%,17.4% and 40%, respectively for the mono specific assay versus 91.7%, 37.5%,13% and 46.7% for the bi specific assay ( X 2=0.0139,P 0.05).No cross reaction in 41 sera from parasitic infections other than cysticercosis and no false reaction in 50 healthy individuals were observed.CONCLUSION:The McAb based mono specific and bi specific RIHA showed high sensitivity and specificity in CAg detections as well as in efficacy evaluations,while the latter was simpler in reagent preparation with a more stable and longer duration of storage.