中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (3): 214-217.
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邓珊珊,严晓岚,宋昌存
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摘要： 采用SDS－PAGE和ELIB技术分析马来丝虫成虫（MAA）和微丝蚴（MFA）可溶性抗原。马来丝虫成虫和微丝蚴采自感染沙鼠腹腔。分析结果表明，MFA蛋白组分含有沙鼠腹腔液蛋白组分（64－67kDa和56－58kDa）。健康沙鼠血清与MFA作ELIB，可见3条淡反应带（60kDa、74kDa和100kDa），与MAA无反应带可见。MAA蛋白组分中的42kDa和14．5kDa可被感染6个月的阳性沙鼠血清识别，而不被阴性沙鼠血清识别。
关键词: 马来丝虫, 酶联免疫印迹技术, 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳
Abstract: Soluble antigens of adult worms (MAA) and microfilariae (MFA)were analysed by means of SDS PAGE and enzyme linked immunoboltting (ELIB).The adult worms and microfilariae were collected from abdominal cavity of Mongolian jirds infected with Brugia malayi .The results indicated that MFA shared 64-67 kDa and 56-58 kDa protein components with the peritoneal fluid of the normal jirds.Sera from normal jirds were found to re cognize 60 kDa,74 kDa and 100 kDa fractions of MFA,but did not recognize any bands of MAA.Sera from positive jirds which were infected with B.malayi for 6 months could recognize 42 kDa and 14.5 kDa of MAA,but sera from negative jirds could not.The results indicated that the 42 kDa and 14.5 kDa protein components of MAA might be specific diagnostic antigen fractions.
中图分类号:
R532.150.4
邓珊珊,严晓岚,宋昌存. 马来丝虫病特异性诊断抗原研究[J]. 中国寄生虫学与寄生虫病杂志, 1994, 12(3): 214-217.
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