中国寄生虫学与寄生虫病杂志 ›› 1994, Vol. 12 ›› Issue (3): 172-177.

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中国大陆钉螺等位基因位点研究

张仪,冯婷,George MDavis   

  1. 中国预防医学科学院寄生虫病研究所; 美国费城自然科学院
  • 出版日期:1994-08-31 发布日期:2017-01-06
  • 基金资助:
    美国国立卫生研究院(NIH)资助

STUDY ON ALLELE FREQUENCY IN ONCOMELANIA FROM THE MAINLAND OF CHINA 

  • Online:1994-08-31 Published:2017-01-06

摘要: 检测安徽、上海、江苏、浙江、江西、湖南、湖北、四川、云南9省(市)的9地代表性钉螺的AAT、ACPH、AK、AO、APH、CK、EST、GDH、GPI、G6PD、HBD、ISDH、LAP、LDH、ME、MDH、MPI、NADD、OCT、PGM、6PGD、SDH、SOD、XDH24种酶。检得40个等位基因位点,117个等位基因;其中变异最大的位点是GPI和PGM-Ⅰ。22个位点的等位基因在3个以上。多态位点酶有14个,占所测酶的58.33%。表明中国大陆钉螺存在遗传多态现象。同时发现PGM、MDH酶是中国大陆钉螺及日本血吸虫共有的多态位点酶,为虫、螺两者相互关系的研究提供新的线索。

关键词: 钉螺, 等位基因, 多态位点, 遗传多态

Abstract: Nine populations of Oncomelania,field collected from Anhui,Shanghai ,Jiangsu,Zhejiang,Jiangxi,Hunan,Hubei,Sichuan and Yunnan were studied by horizontal starch gel electrophoretic method with 24 enzyme systems(AAT,AcPH,AK,AO,APH,CK,EST,GDH,GPI,G6PD,HBD,ISDH,LAP,LDH,ME,MDH,MPI,NADD,OCT,PGM,6PGD,SDH,SOD,XDH)analyzed.40 loci and 117 alleles were detected in the Oncomelania.Both of GPI and PGM Ⅰ,with 7 alleles,were the most variable loci.22 loci had more than 3 alleles each.Of 40 loci examined in the 24 isozyme systems,14 were found to be polymorphic,the proportion of multilocus enzymes being 58.3%.Our results showed that the genetic polymorphism existing in the populations of Oncomelania in the mainland of China.PGM and MDH,were found in both the populations of Oncomelania and strains of Schistosoma japonicum in the mainland of China.The results provided a new idea for studying snails and Schistosoma.Also,we found that there might be some correlation between the polymorphic locus and the feature of the shell of Oncomelania snail.

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