关键词: 卫氏并殖吸虫, 斯氏狸殖吸虫, 重复DNA序列, 限制性内切酶

Abstract: The genomic DNA of the two lung flukes (P. westermani and P. skrjabini)from six areas was digested separately by three restriction endonucleases BamHI,Hae Ⅲ and Hind Ⅲ (followed by agarose gel electrophoresis. Repetitive DNA were visusalized as distinct bands in stained gels. Restriction fragment length differences were detected between the two species. The majority of bands of repetitive DNA were similar with minor differences within the same species collected from different areas,upon digestion with certain restriction endonucleases.The results demonstrated that the technique for demonstrating the band pattern of repetitive DNA with three restriction endonucleases can be used for the identification of P. westermani and P. skrjabini and the geographic relationship of the same species from different areas (Figs. 1 - 3).