中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (1): 8-11.
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李本文,卢惠霖,姚开泰,刘多
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摘要: 本研究首次报道了赫坎按蚊种团内亲缘种间基因组中重复DNA限制酶切长度差异。研究的蚊种包括中华按蚊、嗜人按蚊、凉山按蚊、克劳按蚊和小宽按蚊等5个亲缘种。制备每种蚊的基因组DNA,分别以BglⅡ、HaeⅡ和PstⅠ等3种限制性核酸内切酶消化,琼脂糖电泳分离,比较重复序列DNA的带型.结果显示5个亲缘种的重复DNA的限制酶切片段的带型有明显的差异.该法敏感可靠,可作为亲缘种间鉴别的一个方法。
关键词: 赫坎按蚊种团, 基因组DNA, 限制酶切长度差异
Abstract: Genomic DNA were prepared from 5 sibling species of Anopheles hyrcanus group in-eluding An. sinensis (ASS), An. anthropophagus (ALA), An. liangshanensis (ALS),An: crawfordi (ACW) and An. xiaokuanus (AXK). High molecular weight DNA from each species were cut with three restriction endonuleases (Bgl Ⅱ, Hae Ⅲ and PstⅠ) and the DNA fragments analysed by agarose gel electrophoresis and ethidium bromide st?.inin.g. Three enzymes (Bgl Ⅱ, Hae Ⅲ and PstⅠ) produced unique fragments in all sibling species tested. A diagnostic restriction fragment length of DNA from 5 sibling species of An. hyrcanus group may be derived from a single restriction enzyme pattern (i. e. Bgl Ⅱ). The results demonstrated that the restriction fragment length differences of repetitive DNA could used as a tool to distinguish sibling species of An. hyrcanus group.
李本文,卢惠霖,姚开泰,刘多. 赫坎按蚊种团内各亲缘种间基因组重复DNA的限制酶切长度差异[J]. 中国寄生虫学与寄生虫病杂志, 1991, 9(1): 8-11.
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