中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (1): 65-67.

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猪囊尾蚴冰冻切片免疫酶染色试验诊断脑囊虫病的初步研究

姜洪杰,卞英华,王凤芸   

  1. 首都医学院寄生虫学教研室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1991-02-28 发布日期:2017-01-09

DIAGNOSIS OF NEUROCYSTICERCOSIS BY IMMUNOENZYMATIC STAINING TECHNIQUE (IEST) WITH FROZEN SECTIONS OF CYSTICERCUS CELLULOSAE

  • Received:2017-01-09 Revised:2017-01-09 Online:1991-02-28 Published:2017-01-09

摘要: 应用猪囊尾蚴冰冻切片作为抗原进行免疫酶染色试验。脑囊虫病人的检出率为98.2%(55/56);50例健康人、23例华支睾吸虫病人和26例并殖吸虫病人的假阳性率和交叉反应均为0,但细粒棘球蚴病人血清的交叉反应为18.1%(4/22)。结果表明,该法是一种特异性强、敏感性高、经济与有效的脑囊虫病血清学诊断方法。

关键词: 猪囊尾蚴, 冰冻切片, 免疫酶染色, 脑囊虫病

Abstract: This article deals with the sensitivity and specificity of IEST in neurocysticercosis with frozen sections of Cysticerus cellulosae as antigens. 55 out of 56 ccses (98.2%) of neurocysticercosis showed positive reaction. All serum specimens from healthy human controls (50 cases), clonorchiasis (23 cases) and paragonimiasis (26 cases) were negative, but 18% (4/22) of serum specimens from echinococcosis revealed positive reaotions. The results indicate that IEST is specific, sensitive, economical and easy to perform for the diagnosis of neurocysticercosis.