中国寄生虫学与寄生虫病杂志 ›› 1990, Vol. 8 ›› Issue (2): 110-112.

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钉螺转氨酶的初步研究

王根法,宋庚明   

  1. 中国预防医学科学院寄生虫病研究所
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1990-05-31 发布日期:2017-01-09

PRELIMINARY STUDIES ON TRANSAMINASE OF ONCOMELANIA SNAIL

  • Received:2017-01-09 Revised:2017-01-09 Online:1990-05-31 Published:2017-01-09

摘要: 纸层析法证明丙氨酸、天门冬氨酸及精氨酸经过钉螺匀浆作用后可使α-酮戊二酸转变成谷氨酸,说明钉螺存在谷-丙、谷-草及谷-精转氨酶。比色法也测得谷-草转氨酶和谷-丙转氨酶的活力。溴乙酰胺对上述两酶活力无明显影响,而烟酰苯胺有抑制钉螺谷-丙转氨酶作用。小剂量溴乙酰胺与烟酰苯胺盐酸盐伍用,无增强杀螺作用。

关键词: 溴乙酰胺, 烟酰苯胺, 钉螺, 转氨酶, 谷-草转氨酶, 谷-丙转氨酶

Abstract: By paper chromatography, the tissue homogenate of Oncomelania snails was shown to form glutamic acid at the expense of a-ketogiutarate pms asparuc acia,aialmine otarginine respectively. The existence of alanine-glutamate, aspartate-glutamate and arginine-glutamate transaminase in Oncomelania snail was demonstrated.By using colorimetric method, the activity of aspartate-glutamate transaminase(GOT) and alanine-glutamate transaminase (GPT) of Oncomelania snail was 1.64±0.01 and 0.99 ± 0.01μmol/h·mg protein respectively. GOT and GPT were not inhibited by 2ppm bromoacetamide, but the activity of GPT was suppressed (40 %) by 2ppm nicotinanilide. A combination of 0.5ppm bromoacetamide and 0.5ppm nicotinanilide had no synergetic molluscicidal effect (Fig. 1).