中国寄生虫学与寄生虫病杂志 ›› 1987, Vol. 5 ›› Issue (4): 278-280.

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三平正并殖吸虫囊蚴的生物学与蛋白质区带初步观察

沈一平,张耀娟   

  1. 南京医学院寄生虫学教研室
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1987-11-30 发布日期:2017-01-12

PRELIMINARY OBSERVATION ON SOME ASPECTS OFBIOLOGY AND PROTEIN BANDS OF METACERCARIAE OFEUPARAGONIMUS CENOCOPIOSUS

  • Received:2017-01-12 Revised:2017-01-12 Online:1987-11-30 Published:2017-01-12

摘要: 本文对三平正并殖吸虫囊蚴进行了生物学和生物化学的特性观察。结果表明在长江华溪蟹中的三平正并殖吸虫囊蚴和卫氏井殖吸虫囊蚴在生物学与生物化学方面有明显的差别。诸如三平正并殖吸虫囊蚴绝大多数寄生于蟹心脏,而极少在蟹肌肉等部位寄生。三平正并殖吸虫和卫氏并殖吸虫在蟹体内的囊蚴感染率分别为42.8和100%。这两种囊蚴经腹腔感染大白鼠,其感染数分别为3/6和10/10,而虫体回收率则分别为4.5~8.8%及22~72.2%。应用盘状电泳和等电点聚焦电泳测定蛋白质区带模式证明两种囊蚴间有明显的差别.

关键词: 三平正并殖吸虫, 卫氏并殖吸虫, 感染率, 长江华溪蟹, 生物学特性, 蛋白质组分, 初步观察, 囊蚴, 盘状电泳, 寄生虫学

Abstract: Some biological and biochemical characteristics of metacercaria of Euparagonimus ce-nocopiosus obtained from crabs in the mountainous area of south Anhui, were compared with those of Paragonimus westermani from the same batch of crabs.The metacercariae of E. cenocopiosus prefer the location in heart rather than in the muscle of crabs. The average infection rate of crabs was 42.8% by E. cenocopiosus, while that by P. westermani was 100%. The infection rate of rats with E. cenocopiosus was 3/6 and 10/10 with P. westermani and the recovery rate of worm of these two species was 4.5-8.8% and 22-72.2% respectively. The number and pattern of protein bands of metacercaria of these two species by disc-PAGE and IEF technics were different.