中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (3): 195-197.

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开放性固体平板培养苏云金杆菌及现场灭蚊的效果

陈世夫,肖永昌,吕建芳   

  1. 山东省莱阳县卫生局科研组
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-08-31 发布日期:2017-01-12

OPEN SOLID PLATE MEDIUM CULTURED BACILLUS THURINGIENSIS AND ITS MOSQUITOCIDAL EFFECT IN FIELD APPLICATION

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-08-31 Published:2017-01-12

摘要: 研究使用开放性固体培养法生产苏云金杆菌血清型H-14制剂60批,总产量达2.000kg。效价可达600IU/mg,经现场灭蚊效果考核及安全性实验,表明其与国外Sandoz菌液灭蚊幼的效果相似。经过一个蚊虫季节的现场应用效果观察,可使成蚊密度较对照区下降95.0%。

关键词: 苏云金杆菌, 平板培养, 生物效价, 平板法, 毒力测定, 实验区, 成蚊密度, 现场应用, 灭蚊效果, 固体

Abstract: This paper reported on the cultivation of Bacillus thuringiensis serotypc H-14 with open solid plate medium. It was found that the toxicity of this preparation was onefold higher than that cultured in the shaking bottle. 60 batches have produced, amounting to 2,000kg. The titre of the preparatien was 600 ITU/ing. Evaluation of its mo-quitocidal effect on the spot showed that the efficacy of the preparation was similar to that of the Sandoz bacteria fluid, reducing the mosquito density to 92.6% as compared with that in a control area.