中国寄生虫学与寄生虫病杂志 ›› 1985, Vol. 3 ›› Issue (2): 120-121.

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血吸虫干卵抗原片的环卵沉淀试验

汤淡云,龚玲妹,郑思民   

  1. 上海市血吸虫病防治研究所
  • 收稿日期:2017-01-12 修回日期:2017-01-12 出版日期:1985-05-31 发布日期:2017-01-12

STUDIES ON CIRCUMOVAL PRECIPITIN TEST (COPT) USING SCHISTOSOME EGGS DRIED ON SLIDES AS ANTIGEN

  • Received:2017-01-12 Revised:2017-01-12 Online:1985-05-31 Published:2017-01-12

摘要: 利用血吸虫卵抗原中的有效物质的耐热特性,将热处理超声干卵挑入载玻片上或凹玻片凹孔内水滴中,然后直接加温使之干涸,制成了环卵沉淀试验(COPT)的抗原片。应用此“抗原片”检测粪检血吸虫卵阳性患者104例,COPT阳性率为96.15%;检测正常成人和15岁以下儿童63例,全部阴性。

关键词: 环卵沉淀试验, 抗原片, 血吸虫卵, 血吸虫病防治, 干卵, 常规法, 免疫诊断, COPT, 敏感性和特异性, 有效物质

Abstract: As the effective components of schistosome egg antigen were heat-stable, the "antigen slide" on which the heated and ultrasonicated ova suspended in distilled water were dried by heating was used for COPT. Sera from 104 patients passing schistosome eggs gave a COPT positivity rate of 96.2%. The "antigen slide" COPT is recommended because its manipulation was faster and simpler than that of routine COPT, and positive slides could be kept for a comparatively longer period of time.