关键词: 沉淀法, 醋酸纤维膜, 实验研究, 微量免疫, 按蚊胃血标本, 寄生虫病研究所, 扩散法, 纤法, 抗血清, 阳性率

Abstract: This paper reports on the results of application of the cellulose acetate micro immunodiffusion to identify the host source of anopheline mosquito blood meals. For comparison, the precipitin test was done simultaneously.129 specimens of the 2-3 stage of anopheline mosquito blood meals were identified by cellulose acetate diffusion test and precipitin test, the positive rates being 98.4% and 89.9% respectively. For 121 specimens from the 4-5 stage of mosquito blood meals, the postivity were 93.4% and 19.8% respectively. When tested against 102 specimens of mosquito blood meals collected from cowsheds and pig fences, the former technique showed 100% specificity while the latter only 83.3%. The results indicated that the cellulose acetate diffusion test is more sensitive and specific thap the precipitin test. In addition, the method is also rapid and simple for identifying mosquito blood meals.