三苯双脒对小鼠感染3个旋毛虫分离株的疗效观察

摘要/Abstract

摘要： 目的观察三苯双脒对感染3个分离株旋毛虫小鼠的疗效。方法将144只昆明小鼠随机均分为A组和B组，每组72只。A组小鼠再随机均分为12组，即河南分离株（以下简称河南株）、云南分离株（以下简称云南株）和黑龙江分离株（以下简称黑龙江株）旋毛虫感染组各4组，每组小鼠各感染旋毛虫分离株幼虫200条/只，感染后5 d（即成虫期）分别顿服三苯双脒10、20和30 mg/kg，同时设未服药对照组。B组的分组和感染同A组，感染后53 d（即幼虫成囊期）分别灌胃三苯双脒100、200和300 mg/（kg·d），1次/d×7 d。A组治疗后2 d处死，计数小肠内成虫数。B组治疗后10 d处死，剖取全部膈肌，经消化液消化后计数幼虫。计算各组平均虫数和减虫率。结果 A组中，河南株和云南株各治疗组平均虫数均低于对照组（P＜0.01），河南株3个治疗组的减虫率分别为39.0%、57.9%和86.0%，云南株的减虫率分别为34.9%、69.3%和92.2%，分别随服用三苯双脒剂量的增加，减虫率呈增高的趋势，其中30 mg/kg组各有2只鼠被治愈。黑龙江株10 mg/kg组的平均虫数与对照组的差异无统计学意义（P>0.05），其他2个剂量组平均虫数均显著少于对照组（P＜0.01），3组的减虫率分别为27.9%、57.4%和60.7%，亦随服用三苯双脒剂量的增加，减虫率呈增高的趋势。B组各治疗组小鼠的平均虫数均低于对照组（P＜0.05），河南株的减虫率分别为57.8%、75.4%和87.5%，云南株的分别为74.5%、92.4%和99.1%，黑龙江株的分别为50.5%、53.3%和61.6%。可见3个旋毛虫感染组均随服药剂量的增加，减虫率相应增高。30 mg/kg剂量组中，云南株的减虫率与河南株的和黑龙江株的比较，差异有统计学意义（P＜0.05）。结论三苯双脒对小鼠体内3个地域分离株旋毛虫成虫和成囊期幼虫均有一定的疗效，对云南株旋毛虫疗效更明显。

关键词: 三苯双脒, 地域株, 旋毛虫, 成虫, 成囊期幼虫, 疗效

Abstract: Objective To evaluate the efficacy of tribendimidine （TBD) against 3 geographical isolates of Trichinella spiralis in mice. Methods Isolates of T. spiralis from Henan (hereinafter referred to as HnT.s), Yunnan (referred to as YnT.s) and Heilongjiang (referred to as HljT.s) were used in the study. 144 Kunming strain mice were divided into 2 groups: 72 mice in group A (adult stage, treatment at 5 d after infection), and 72 mice in group B (encapsulated larva stage, treatment at 53 d after infection). Group A was further divided equally into 12 sub-groups. Mice in every 3 sub-groups were each infected orally with 200 T. spiralis larvae of the 3 isolates respectively, and the remained 3 sub-groups served as untreated control. Mice in the 3 sub-groups infected with one isolate were orally treated with TBD at a single dose of 10, 20， and 30 mg/kg, respectively. Group B was treated as group A but with a course of TBD once daily at a dose of 100, 200， and 300 mg/(kg·d) for 7 d, respectively. Mice in group A were sacrificed 2 d post-treatment and adult worms were recovered from the small intestine and counted. Those in group B were sacrificed 10 d after completion of 7 d treatment. The intact diaphragm was removed and digested for collecting larvae. Worm burden and worm reduction of each treated sub-group were calculated and statistically compared with the respective control. Results In group A, the mean worm burden in the treated sub-groups infected with HnT.s and YnT.s were all significantly lower than that of the controls (P＜0.01), with a mean worm reduction rate of 39.0%, 57.9%， and 86.0% in HnT.s sub-groups, and of 34.9%, 69.3%， and 92.2% in YnT.s sub-groups, respectively, showing an increase with the dosage, 2 mice in each of the 30 mg/kg sub-groups were cured. The worm burden in the 10 mg/kg of HljT.s subgroup was similar to that of the control (P>0.05), but was significantly lower in the other 2 sub-groups than that of the controls (P＜0.01). The worm reduction rate in the 3 sub-groups was 27.9%, 57.4%, and 60.7%, respectively. In all treated sub-groups of group B, the mean worm burden was significantly lower than that of the controls (P＜0.05), with a mean worm reduction rate of 57.8%, 75.4%, and 87.5% in HnT.s sub-groups, of 74.5%, 92.4%, and 99.1% in YnT.s sub-groups, and of 50.5%, 53.3%, and 61.6% in HljT.s sub-groups, respectively, with the 3 dosages. Conclusion Tribendimidine shows adequate efficacy on Trichinella spiralis adults and on encapsulated larvae of the 3 geographical isolates in mice, with better effect on Yunnan isolate.

Key words: Tribendimidine, Geographical isolate, Trichinella spiralis, Adult, Encapsulated larva, Efficacy

高云，杨晓东，王丽娜，陈晓宁，李丽娜，赵香菊，刘丹. 三苯双脒对小鼠感染3个旋毛虫分离株的疗效观察[J]. 中国寄生虫学与寄生虫病杂志, 2012, 30(4): 8-290-293.

GAO Yun，YANG Xiao-dong，WANG Li-na，CHEN Xiao-ning，LI Li-na，ZHAO Xiang-ju，LIU Dan. Efficacy of Tribendimidine Against Three Isolates of Trichinella spiralis in Mice[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2012, 30(4): 8-290-293.

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三苯双脒对小鼠感染3个旋毛虫分离株的疗效观察

Efficacy of Tribendimidine Against Three Isolates of Trichinella spiralis in Mice

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