中国寄生虫学与寄生虫病杂志 ›› 2006, Vol. 24 ›› Issue (增刊): 16-s75.

• 讲座 • 上一篇    下一篇

蚊虫采集、保存及标本制作

朱淮民   

  1. 第二军医大学病原生物学教研室, 上海 200433
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-12-30 发布日期:2006-12-30

Collecting and Preserving Mosquitoes: Tools and Techniques

ZHU Huai-min   

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-12-30 Published:2006-12-30

摘要: 本文简要介绍蚊虫采集和保存的技术和方法。常见种类的蚊虫很容易在自然环境中采集到,而一些珍稀蚊虫种类,必须在采集、制作成标本后妥善保存,以供进一步鉴定虫种或研究之用。在鉴定虫种时,通常需要检查虫种的细小解剖结构,还须借助放大镜和显微镜进行观察,若这些结构被破坏,则无法鉴定虫种。某些特殊复合种群的蚊虫采集有助于研究其生物学、生理学、生态学、分子和系统发育生物学,许多传统的采集和保藏方法及技术较易掌握,而保存采集标本用于分子生物学的研究,则是近几十年才出现的。

关键词: 蚊虫, 采集, 保藏, 标本

Abstract: This lecture provides a summary of the methods and techniques used by professionals to collect and preserve mosquito specimens for study and teaching. Many species of mosquitoes can be collected most easily in their natural environments. However, some species must be collected and properly preserved before they can be identified. Because correct identification seldom is easy, it is important that specimens be preserved in the best condition possible. The identification of a particular insect usually requires examination of minute details of its anatomy with the aid of a hand lens or microscope. If these details on a specimen are concealed, missing, or destroyed because of improper handling or preservation, identification is made difficult or impossible, and information about the species to which it belongs cannot be made available. Specific groups or species may be collected to acquire material for biological, physiological, ecological, molecular, and systematic studies. While many of the methods covered here, such as samples collecting, pinning, slide?鄄mounting, and other techniques have become available everywhere; but for molecular studies, only in the recent years or decades the advancing technologies developed.

Key words: Mosquito, Collection, Preservation, Specimen