伯氏疟原虫氯喹敏感株和抗性株在疟色素形成和致病性上的差异

中国寄生虫学与寄生虫病杂志 ›› 1999, Vol. 17 ›› Issue (1): 5-20.

伯氏疟原虫氯喹敏感株和抗性株在疟色素形成和致病性上的差异

严继舟¹;宋关鸿¹;龚志锦²;陆月良³

第二军医大学1　寄生虫学教研室 2　病理学教研室 3　中心实验室电镜室上海 200433

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-02-28 发布日期:1999-02-28

DIFFERENCES IN HAEMOZOIN PRODUCTION AND PATHOGENICITY BETWEEN CHLOROQUINE SENSITIVE AND CHLOROQUINE RESISTANT STRAINS OF PLASMODIUM BERGHEI

YAN Jizhou ¹;SONG Guanhong¹;GONG Zhijing²;LU Yueliang ³

1 Department of Parasitology;Second Military Medical University;Shanghai 200433 2 Department of Pathology;Second Military Medical University;Shanghai 200433 3 Laboratory of Electron M icroscopy , Second M ilitary Medical University , Shang hai 200433

Received:1900-01-01 Revised:1900-01-01 Online:1999-02-28 Published:1999-02-28

摘要/Abstract

摘要： 　　目的：研究伯氏疟原虫氯喹敏感株（Ｎ）和抗性株（ＲＣ）在疟色素形成和致病性上的差异。方法：用伯氏疟原虫氯喹敏感株和抗性株分别感染ＩＲＣ小鼠。实验分为５组：正常对照组（ＮＣ），氯喹治疗对照组（ＣＣ），Ｎ株感染组（Ｎ），ＲＣ株感染组（ＲＣ）和ＲＣ株感染加氯喹治疗组（ＲＣＣ），比较各组间末梢血中疟原虫的形态、原虫血症、肝脏组织学及超微结构的改变。结果：Ｎ组肝细胞损害较严重，细胞内线粒体肿胀融合，溶酶体增多，肝血窦内疟原虫少见。ＲＣ组炎症反应较突出，主要为单核细胞浸润以及枯否细胞活跃，大滋养体和裂殖体在肝血窦滞留；肝细胞损害较轻，表现为线粒体增生、肿胀及空泡化。Ｎ株疟原虫富含内食物泡，其内有疟色素颗粒，被寄生红细胞结构较完整；而ＲＣ株疟原虫外食物泡较多，位于疟原虫外围的红细胞胞质内，被寄生的红细胞呈蜂窝样改变，食物泡内无疟色素。结论：ＲＣ株疟原虫可能改变了原敏感株（Ｎ株）对血红蛋白的摄取和消化方式，从而阻碍了疟色素形成；Ｎ株和ＲＣ株疟原虫可能因诱导宿主免疫反应的明显差异，导致ＲＣ株较Ｎ株致病力为弱

关键词: 伯氏疟原虫, 氯喹抗性, 疟色素, 致病性, 外食物泡, 炎性反应

Abstract: 　AIM: To better understand the differences in haemozoin formation and pathogenicity between chloroquine sensitive(N) and chloroquine resistant(RC) strains of Plasmodium berghei. METHODS: IRC mice were grouped as follows: group XbaⅠ (Normal control, NC), group Ⅱ (control treated with chloroquine alone, CC), group Ⅲ (mice infected with N strain), group Ⅳ (mice infected with RC strain,RC) and group Ⅴ (mice infected with RC strain and treated with chloroquine, RCC). Morphologic features of the parasites, parasitaemia, histological and ultrastructural changes of livers among the groups were compared. RESULTS: Severe damages of the hepatic cells of the N group including increased lysosomes and swollen and fused mitochondria were detected. On the contrary, the prominent features in liver section of the RC group were inflammatory cell (notably mononuclear) infiltration and Kupffer cell activation. Numerous trophozoites and schizonts were sequestrated in hepatic sinusoids and less degeneration of parenchymal cells was found except for some swollen and vacuolated mitochondria. Internal food vacuoles containing haemozoin were found in the parasites of the N group, whereas external food vacuoles without haemozoin granules were arranged in foamy appearance within the parasitized cell of the RC group. CONCLUSION: P.berghei RC strain may modify the mode of ingestion and degradation of hemoglobin in the parasites, resulting in impeding haemozoin formation. The difference in virulence between the N and RC strain of P.berghei is probably attributed to the significant differences in the induction of immune response of the host.

Key words: Plasmodium berghei, chloroquineresistance, haemozoin, pathogenicity, external food vacuole, inflammatory response

严继舟;宋关鸿;龚志锦;陆月良. 伯氏疟原虫氯喹敏感株和抗性株在疟色素形成和致病性上的差异[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(1): 5-20.

YANJizhou;SONGGuanhong;GONGZhijing;LUYueliang . DIFFERENCES IN HAEMOZOIN PRODUCTION AND PATHOGENICITY BETWEEN CHLOROQUINE SENSITIVE AND CHLOROQUINE RESISTANT STRAINS OF PLASMODIUM BERGHEI[J]. Chinese Journal of Parasitology and Parasitic Diseases, 1999, 17(1): 5-20.

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