中国寄生虫学与寄生虫病杂志 ›› 1997, Vol. 15 ›› Issue (5): 266-268.

• 论著 • 上一篇    下一篇

约氏疟原虫53kDa抗原对小鼠的保护性免疫作用

王春莉; 薛采芳; 张荣庆; 甄荣芬   

  1. 第四军医大学寄生虫学教研室
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-10-30 发布日期:1997-10-30

PROTECTIVE IMMUNITY IN MICE AFTER IMMUNIZATION WITH PLASMODIUM YOELII 53kDa ANTIGEN

Wang Chunli; Xue Caifang; Zhang Rongqing; Zhen Rongfen   

  1. Departmentof Parasitology; The Fourth Military Medical University; Xi'an710032
  • Received:1900-01-01 Revised:1900-01-01 Online:1997-10-30 Published:1997-10-30

摘要: 目的 :探讨白细胞介素 - 2 ( IL- 2 )与约氏疟原虫 ( P.y)纯化抗原 53k Da抗原的免疫作用。方法 :用 53k Da抗原加福氏完全佐剂 ( FCA)腹腔注射免疫 BALB/ c小鼠 3次 ,每鼠再用 P.y非致死株 10 5感染红细胞攻击。观察免疫过程中和原虫攻击后第 6、12、18d脾细胞的刀豆球蛋白( Con A)或抗原体外诱生的IL - 2水平变化及其与血中原虫率的关系。结果 :用 53k Da抗原免疫鼠 ,免疫结束时的 IL- 2诱生水平明显高于未免疫对照组 ,原虫攻击感染后免疫组的原虫率峰值为1.9% ,明显低于佐剂对照组的 2 5.4 %和未免疫组的 2 6.1%。结论 :P.y 53k Da抗原可产生明显的保护性免疫。

关键词: 约氏疟原虫, 保护性抗原, 白细胞介素-2, 原虫血症

Abstract: AIM:To study the protective immunity of Plasmodium yoelii(P.y) 5 3k Da antigen.
METHODS:BALB/c mice were immunized with3doses of P.y 53k Da protective antigen and then
challenged ip with nonvirulent P.y infected erythrocytes1 0 days after the third im- munization.
Observations on Con A or P.y antigen- induced production of IL - 2 by spleen cells in vitro were
carried out during vaccination period and at days6 ,1 2 ,1 8after challenge. The relationship
between the levels of IL- 2 and parasitaemia was analysed. RESULTS: The IL-2 levels induced by P. y 53 kDa antigen and ConA were much higher in immunized group than in the unimmunized groups before challenge. The peak rate of parasitaemia of the immunized group was 1. 9% , which is much lower than that of 26. 1% in the unimmunized mice and 25. 4% in mice immunized with Freund’s complete adjuvant. CONCLUSION: P. y 53kDa antigen can induce a marked immune protection in mice against erythrocytic stage of P.yoelii.

Key words: Plasmodium yoelii, protective antigen, interleukin- 2, parasitaemia