中国寄生虫学与寄生虫病杂志 ›› 1993, Vol. 11 ›› Issue (4): 241-243.

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日本血吸虫成虫31/32 kDa蛋白的纯化及保护性免疫力的研究

沈定文,李雍龙,韩家俊,石佑恩   

  1. 咸宁医学院寄生虫学教研室; 同济医科大学寄生虫学教研室; 同济医科大学寄生虫学教研室; 同济医科大学寄生虫学教研室
  • 收稿日期:2017-01-06 修回日期:2017-01-06 出版日期:1993-11-30 发布日期:2017-01-06

PURIFICATION OF 31/32 kDa PROTEINS OF ADULT SCHISTOSOMA JAPONICUM AND STUDIES ON THEIR PROTECTIVE IMMUNITY

  • Received:2017-01-06 Revised:2017-01-06 Online:1993-11-30 Published:2017-01-06

摘要: 本文采用制备型聚丙烯酰胺凝胶电泳(SDS-PAGE)和电渗析方法分离纯化日本血吸虫成虫 31/32 kDa 蛋白(Sj 31/32蛋白)。SDS-PAGE、免疫印迹试验(EITB)和酶联免疫吸附试验(ELISA)检测结果表明采用该方法分离纯化的 Sj 31/32 蛋白纯度高,且具有较高的活性。用纯化的 Sj 31/32 蛋白加福氏佐剂免疫的小鼠,不仅可减少虫负荷(减虫率 28.1%),还可降低血吸虫成虫的产卵量(减卵率 71.4%),抑制虫卵肉芽肿的形成。结果提示:Sj 31/32 蛋白具有较高的减卵率,可望作为混合多价疫苗候选组分。

关键词: 日本血吸虫, 成虫/, kDa蛋白, 分离和纯化, 保护性免疫

Abstract: Schistosoma japonicum adult worm 31/32 kDa proteins (Sj 31/32) were separated on polyacrylamide slab gels and purified by electrophoretic elution. These purified proteins were used to immunize mice in order to observe their protective immunity against challenge. The results of SDS-PAGE,EITB and ELISA indicated that the 31/32 kDa proteins separated and purified by means of these methods were pure and active. It was assumed that Sj 31/32 proteins could reduce worm burden and inhibit the fecundity of schistosome and formation of egg granuloma. The results suggested that 31/32 kDa 5. japonicum proteins might be an important component of a multivalent vaccine against schistosomiasis japonica (Figs. 1 - 4).