中国寄生虫学与寄生虫病杂志 ›› 1992, Vol. 10 ›› Issue (4): 258-262.

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溴乙酸胺对钉螺卵发育的影响

张仪,郭源华   

  1. 中国预防医学科学院寄生虫病研究所
  • 收稿日期:2017-01-07 修回日期:2017-01-07 出版日期:1992-11-30 发布日期:2017-01-09

STUDY ON THE EFFECT OF BROMOACETAMIDE UPON THE DEVELOPMENT OF SNAIL EGGS

  • Received:2017-01-07 Revised:2017-01-07 Online:1992-11-30 Published:2017-01-09

摘要: 0.0343ppm-0.067ppm溴乙酰胺浸泡钉螺卵后,从单细胞期发育到原肠胚期,其形态出现畸形;但发育到担轮-缘膜幼虫期和完成变态的缘膜幼虫期螺卵出现畸形胚所需浓度比囊胚期、原肠胚期螺卵出现畸形胚的浓度为高。溴乙酰胺浸泡螺卵,时间相同,浓度越高,滞育率越高,而时间越长,阻碍螺卵发育所需浓度越低,孵化率也越低。经溴乙酰胺作用后,雌螺产卵量明显降低,成熟钉螺卵巢明显萎缩。用~3H标记的溴乙酰胺浸泡钉螺,表明溴乙酰胺可进入钉螺体内的螺卵中,其放射性含量随浸泡钉螺的药物浓度增高而增多。这一结果为以后研究灭螺药对螺卵的作用机制,提供了一种新的方法。

关键词: 溴乙酰胺, 溴[次甲-~H]乙酰胺, 钉螺卵

Abstract: This paper deals with the influence of bromoacetamide on the development of snail eggs,its effect on inducing diapause of snail eggs at different developmental stages (bkstula,gastrula,trochophore and veliger) both at different concentrations of bromoacetamide at the same time and at same concentration at different times.Besides,the oviposition of the snails and the eggs within snails have also been studied.The results are as follows: (1)When the snail eggs were immersed in bromoacetamide at concentrations of 0.034ppm-0.067ppm,deformation of snail eggs appeared from unicelluar to gastrula stages,but it was not observed after the trochophore-veliger stages.When the eggs at different stages were separately immersed in bromoacetamide,they were all found delormed.The molluscicidal concentration for inducing deformation of eggs was higher at the trochophore-veliger and metamophotic stages than that at the blastula and gastula stages (Figs.1-5)(2) After immersed in bromoacetamide solution,the development of the snail eggs were obviously retarded.The effects varied with different concentrations.Within the same exposure time,the higher the bromoacetamide concentration,the higher the diapause rate(3) The longer the immersion time,the lower concentration was needed to delay the development and the lower hatching rate was for the eggs at the same or different str.ges.(4)After treatment with bromoacemide,the average number of eggs laid by a female snail was reduced.(5)By using 3H-labered bromoacetamide,it was found that the radioativity of 3H-labeled bromoacetamide per hundred snail eggs was raised along with the increasing of the molluscicide concentration.It is concluded that direct exposure to low concentration of bromoacetamide could inhibit the development of snail eggs and obviously reduce the hatching rate.Furthermore,the chemical could enter the snail,damage the snail ovary and cause diapause of the snail eggs even though it could not kill the snail directly.It could also reduce the output of snail eggs and delay the reproductive period of the Oncomelania snail.