中国寄生虫学与寄生虫病杂志 ›› 1991, Vol. 9 ›› Issue (2): 86-89.

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日本血吸虫病患者体内自然抗独特型抗体的初步研究

邢益平,洪静婉,吴观陵   

  1. 南京医学院寄生虫学教研室
  • 收稿日期:2017-01-09 修回日期:2017-01-09 出版日期:1991-05-31 发布日期:2017-01-09

PRELIMINARY STUDY OF NATURALLY OCCURRING ANTMDIOTYPIC ANTIBODIES IN SCHISTOSOMIASIS JAPONICA

  • Received:2017-01-09 Revised:2017-01-09 Online:1991-05-31 Published:2017-01-09

摘要: 成用杂交瘤技术所制备的鼠抗日本血吸虫可溶性抗原(SEA)单克隆抗体5B5H腹水经饱和硫酸铵提纯后制成亲和层析柱,血吸虫病人血清过此柱后获得抗Id抗体提取物。经McAb-ELISA测试,提取物与5B5H单抗特异性结合,与另一株抗SEA单抗W_(29)及抗成虫单抗W_(46)均不结合,补体结合试验表明此提取物不含有免疫复合物。SEA可显著竞争抑制Id(5B5H)-抗Id抗体的结合,提示抗Id抗体所针对的5B5H单抗的Id位于抗原结合部位或其邻近。用5B5H-ELISA检测两个不同地区血吸虫病患者的抗Id抗体,阳性率分别为35.1%和60%,与姜片虫病人血清(0/15)、华支睾吸虫病人血清(0/15

关键词: 日本血吸虫病, 单克隆抗体, 补体结合试验

Abstract: In this study, naturally occurring anti-idiotypic antibodies in sera from patients with schistosomiasis japonica were demonstrated by using McAb (5B5H)-ELISA. McAb 5B5H against Schistosoma japonicum soluble egg antigen (SEA) was purified by precipitation With 50%, 40% and 33% saturated (NH4)2SO4 and coupled to CNBr-activated Sepharose 4B (Pharmacia). 5B5H column was loaded with sera from two patients with schistosomiasis japonica and then the bound material was eluated with 3M KCNS. The resulting eluate was further characterized with McAb-ELISA and complement fixation test in order to minimize the possible interference of circulating antigen-antibody complex. The results showed that the eluate contained mainly anti-Id antibodies. In the competitive 5B5H-ELISA tests, SEA markedly inhibited the interaction of 5B5H (Id) and anti-Id anti-bodies, suggesting that the relevant idiotype is within or adjacent closely to antigen binding site of anti-SEA molecule. The anti-Id antibodies were detected using 5B5H-ELISA in patients with schistosomiasis japonica from A and B areas, positive rate of 35.1% (13/37) and 60% (18/30) was obtained respectively. No cross or false reactions were found with sera from patients with fasciolopsiasis buski (0/15), clonorchiasis sinensis (0/15) and normal group of students (0/54) with the exception of one out of 20 patients with paragonimiasis westermani.