为了解石渠县棘球绦虫终末宿主和中间宿主动物活动特点,于2018年6月和8月采用红外感应触发相机在石渠县俄多玛乡日扎村居民定居点附近选取8户家庭观察人与拴养犬之间的互动情况,以及拴养犬与其他动物之间的互动情况。选取4个观察点观察小型哺乳类动物活动规律。红外相机固定在距离地面100 cm左右的电线杆或围栏上,观察记录6 m以内人或动物的有效活动情况。在放置有红外相机并记录到有高原鼠兔和青海松田鼠活动的范围内采集动物样品,进行物种的分类鉴定。对所采集动物样品的细胞色素b基因（Cyt b）进行PCR扩增,并进行双向测序,在GenBank中通过Blastn进行序列比对。结果显示,8户牧民家庭仅有3户共计277 min的视频得到有效记录,每户仅1条拴养犬,均为藏犬,拴养犬距离户主居住点平均在28 m左右,且拴养犬均在户主庭院围栏外,平均每天喂食1次,未发现人或野犬与拴养犬有接触性动作。在高原鼠兔观察点捕获的动物经鉴定为高原鼠兔,其地面活动开始时间为6 : 46,结束时间为20 : 41;活动高峰期出现在9 : 00、12 : 00、15 : 00和19 : 00左右。在青海松田鼠观察点捕获的动物经鉴定为青海松田鼠,其地面活动开始时间为8 : 06,结束时间为20 : 09,其中19 : 00左右活动最为频繁。人与拴养犬的互动以喂食为主,犬被喂食次数少,增加了其捕食小型哺乳类动物的可能性;高原鼠兔活动开始时间早于青海松田鼠,高原鼠兔全天活动更为频繁。

To understand the activities of intermediate and definitive host animals of Echinococcus spp. in Shiqu, Sichuan during June to August 2018, the infrared triggered cameras were used to observe the interaction between human and dogs, and dogs with other animals, within eight households in Rizha village, Odoma township, Shiqu County, Sichuan. Other four sites were selected to observe the activities of small mammals around the selected households. The infrared cameras were installed on poles or fence about 100 cm above the ground to record the activities of people or animals within the range of 6 m. The rodents Ochotona curzoniae and Neodon fuscus within the camera monitor range were collected for the species identification by PCR amplification of cytochrome b gene (Cyt b) and double-stranded DNA sequencing. Total 277 minutes of video were effectively recorded from 3 out of 8 households. The records showed that there was only one leashed Tibetan dog in each household, which moved outside the fence, but within 28 m of house. The dogs were fed once a day averagely. There was no touch caught between people and domestic or wild dogs. The rodents captured by the camera were identified as Ochotona curzoniae and Neodon fuscus by DNA sequencing analysis. The ground activity of Ochotona curzoniae started at 6 : 46 and ended at 20 : 41 with peak at 9 : 00, 12 : 00, 15 : 00 and 19 : 00. The smokey vole Neodon fuscus started to move around at 8 : 06 and ended at 20 : 09 with peak at 19 : 00. Ochotona curzoniae activity began earlier than Neodon fuscus and was aremore active observed throughout the day. The only interaction between dogs and their owners was when dogs were fed. If dogs are fed less, more chance the dogs would prey on small mammals.