了解广西猴类诺氏疟原虫和田鼠巴贝虫感染情况,为诺氏疟原虫和田鼠巴贝虫的防控提供依据。采集广西某猴养殖场猴血样600份,其中食蟹猴330份,猕猴270份,巢式PCR扩增田鼠巴贝虫、诺氏疟原虫的18S rRNA基因,检测感染情况。测序PCR扩增阳性产物,BLAST比对分析,鉴定虫种。结果显示,共16份血样田鼠巴贝虫扩增阳性,感染率为2.7%（16/600）,其中食蟹猴13份,感染率为3.9%（13/330）;猕猴3份,感染率为1.1%（3/270）,差异有统计学意义（P < 0.05）。4份阳性PCR产物序列与田鼠巴贝虫序列（GenBank登录号：KC904078.1）一致性最高,为99.9%。未检测到诺氏疟原虫阳性血样。广西的猴中输入性诺氏疟原虫的定殖风险较低,田鼠巴贝虫的感染率较高。

This study aims to understand the infection status of Plasmodium knowlesi and Babesia microti in farmed monkeys in Guangxi to assess its risk as reservoir host for human infections. Total 600 blood samples were collected from monkeys raised in a farm in Guangxi including 330 Macaca fascicularis and 270 M. mulatta. The infections of B. microti and P. knowlesi were detected by amplifying 18S rRNA of each species using nested PCR. The PCR products of positive samples were DNA sequenced, and then BLAST searched to confirm the species of infected parasite. The nested PCR results showed that 16 monkeys were infected with B. microti with overall positive rate of 2.7% （16/600）. These infected monkeys included 13 Macaca fascicularis and 3 Macaca mulatta with infection rates of 3.9% (13/330) and 1.1% (3/270), respectively. The difference of B. microti infection between these two monkey species was statistically significant (P < 0.05). Four PCR products were sequenced and the obtained sequences possessed 99.9% sequence identity to B. microti (GenBank accession number: KC904078.1). No infection of P. knowlesi was detected in the examined 600 monkey blood samples. The results suggested that infection of B. microti was detectable in the farmed moneys which could be the potential reservoir host for human Babesia infection in Guangxi, while infection of P. knowlesi was not identified in these farmed monkeys.