收集2024—2025年夏秋季在杭州国际旅行卫生保健中心体检的疟疾高流行区首次来华留学生全血样品，采用疟原虫抗原胶体金法、血涂片镜检法及荧光定量PCR（qPCR）法进行检测，对阳性病例进行基础信息收集和流行病学调查。共检测来自尼日利亚等20个国家来华留学生530人，疟原虫抗原胶体金法检出阳性22例，阳性率4.2%（22/530），来源地分别为尼日利亚（16例），坦桑尼亚（2例），几内亚（2例），刚果民主共和国（1例），刚果共和国（1例）。22例阳性者中，qPCR检出恶性疟原虫阳性11例，血涂片镜检出恶性疟原虫阳性1例。流行病学调查显示，所有阳性病例体检时均体温正常、无临床症状。1例镜检阳性后住院治疗，1例体检后因发热住院治疗。疟疾流行区来华留学生存在无症状感染者，应加强疟疾流行区来华留学生疟疾监测和疟疾防治健康教育，早发现早治疗，降低疟疾境外输入风险。

Whole blood samples were collected from international students studied in China for the first time from malaria-hyperendemic regions who underwent health examinations at the Hangzhou International Travel Healthcare Center in summer and autumn during the period from 2024 to 2025, and were tested for Plasmodium using a Plasmodium antigen colloidal gold assay, blood smear microscopy, and fluorescent quantitative PCR assay (qPCR). Basic demographics and epidemiological history were collected from students tested positive for Plasmodium. A total of 530 international students from 20 countries were tested, and Plasmodium antigen colloidal gold assay tested 22 positives, with a positive rate of 4.2% (22/530), including 16 positives from Nigeria, 2 from Tanzania, 2 from Guinea, one from Democratic Republic of Congo, and one from the Republic of Congo. Among the 22 positive cases, 11 cases were positive for P. falciparum detected by qPCR, and 1 case was positive by blood smear microscopy. Epidemiological surveys revealed that all positive individuals were afebrile and asymptomatic during healthy examinations. One microscopically positive case was hospitalized for treatment, and one case was hospitalized for treatment due to fever following healthy examinations. These data indicate asymptomatic cases among international students studied in China from malaria-endemic regions. Enhanced malaria surveillance and health education is required among international students studied in China to facilitate early detection and treatment, so as to reduce the risk of overseas importation of malaria.