旋毛虫虫种核酸鉴定专家共识（2025）

张玺; 崔晶; 刘若丹; 龙绍蓉; 陈木新; 陈家旭; 付宝权; 刘晓雷; 刘明远; 张雅兰; 张红卫; 王中全

doi:10.12140/j.issn.1000-7423.2025.05.002

中国寄生虫学与寄生虫病杂志 >

2025 , Vol. 43 >Issue 5: 607 - 613

DOI: https://doi.org/10.12140/j.issn.1000-7423.2025.05.002

共识与指南

旋毛虫虫种核酸鉴定专家共识（2025）

张玺 ,
崔晶 ,
刘若丹 ,
龙绍蓉 ,
陈木新 ,
陈家旭 ,
付宝权 ,
刘晓雷 ,
刘明远 ,
张雅兰 ,
张红卫 ,
王中全

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1 郑州大学基础医学院病原生物学系，郑州大学寄生虫病研究所，河南郑州 450001
2 中国疾病预防控制中心寄生虫病预防控制所（国家热带病研究中心），上海 200025
3 中国农业科学院兰州兽医研究所，甘肃兰州 730046
4 吉林大学人兽共患病研究所，世界动物卫生组织亚太区食源性寄生虫病协作中心，人畜共患传染病重症诊治全国重点实验室（吉林大学），吉林长春 130015
5 河南省疾病预防控制中心，河南郑州 450000

张玺，男，博士，教授，从事寄生虫学研究。Email: zhangxi@zzu.edu.cn

收稿日期: 2025-07-02

修回日期: 2025-07-27

网络出版日期: 2025-10-27

基金资助

国家重点研发计划“政府间国际科技创新合作”重点专项(2024YFE0199100);中国疾病预防控制控中心2022年度公共卫生领域卫生健康标准化前期研究项目(BZ2022-Q006);国家科技基础条件平台国家寄生虫资源库项目(NPRC-2019-194-30)

收起

Expert consensus on species identification of Trichinella spp. by nucleic acid method (2025)

ZHANG Xi ,
CUI Jing ,
LIU Ruodan ,
LONG Shaorong ,
CHEN Muxin ,
CHEN Jiaxu ,
FU Baoquan ,
LIU Xiaolei ,
LIU Mingyuan ,
ZHANG Yalan ,
ZHANG Hongwei ,
WANG Zhongquan

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1 Department of Pathogen Biology, School of Basic Medical Sciences, Zhengzhou University, Institute of Parasitic Disease of Zhengzhou University, Zhengzhou 450001, Henan, China
2 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Disease Research), Shanghai 200025, China
3 Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China
4 Institute of Zoonotic Diseases of Jilin University, WOAH Collaborating Centre for Food-Borne Parasites from the Asian-Pacific Region, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Disease (Jilin University), Changchun 130015, Jilin, China
5 Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450000, Henan, China

Received date: 2025-07-02

Revised date: 2025-07-27

Online published: 2025-10-27

Supported by

Intergovernmental International Scientific and Technological Innovation Cooperation Projects of National Key Research and Development Program(2024YFE0199100);Preliminary Research Project on Health Standardization in the Public Health Sector of the Chinese Center for Disease Control and Prevention in 2022(BZ2022-Q006);National Science and Technology Basic Conditions Platform, National Parasite Resource Database Project(NPRC-2019-194-30)

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摘要

毛形线虫属（Trichinella spp.，又称旋毛虫属）的幼虫可寄生于人和多种脊椎动物，严重危害人体健康和动物肉类食品安全。旋毛虫虫种繁多，不同虫种的感染性及致病性存在较大差异。近年来，新的旋毛虫虫种被陆续发现，但现有的病原学检查方法（肌肉活检压片镜检法或人工消化法）不能有效区分不同虫种。本共识综合国内外研究进展，就旋毛虫虫种检测的核酸鉴定方法进行详细阐述，以期为旋毛虫分类学研究和旋毛虫病的精准诊断提供参考。

关键词： 旋毛虫属; 旋毛虫病; 虫种鉴定; 多重PCR; 限制性片段长度多态性

本文引用格式

张玺 , 崔晶 , 刘若丹 , 龙绍蓉 , 陈木新 , 陈家旭 , 付宝权 , 刘晓雷 , 刘明远 , 张雅兰 , 张红卫 , 王中全 . 旋毛虫虫种核酸鉴定专家共识（2025）[J]. 中国寄生虫学与寄生虫病杂志, 2025 , 43(5) : 607 -613 . DOI: 10.12140/j.issn.1000-7423.2025.05.002

Abstract

The larvae of Trichinella spp. nematodes parasitize in humans and various vertebrates, which seriously endangers people’s health and animal meat food safety. There are numerous species within the Trichinella genus; and the infectivity and pathogenicity of Trichinella spp. larvae are obviously different. In recent years, new Trichinella species have been discovered consecutively. The available pathogenic examination methods(direct microscopy of biopsy muscles and artificial digestion) could not distinguish the Trichinella species. To provide reference for Trichinella taxonomic researches and the accurate diagnosis of trichinellosis, this consensus elaborate on the nucleic acid methods for the identification of Trichinella spp. based on the latest domestic and international research progress.

Key words： Trichinella; Trichinellosis; Species identification; Multiplex PCR; Restriction fragment length polymorphism

参考文献

[1]	中华人民共和国卫生部. 旋毛虫病的诊断: WS/T 369—2012[S]. 北京: 中国标准出版社, 2012: 1-16.
	Ministry of Health of the People’s Republic of China. Diagnosis of trichinellosis: WS/T 369-2012[S]. Beijing: Standards Press of China, 2012: 1-16. (in Chinese)
[2]	Crisóstomo-Jorquera V, Landaeta-Aqueveque C. The genus Trichinella and its presence in wildlife worldwide: A review[J]. Transbound Emerg Dis, 2022, 69(5): e1269-e1279.
[3]	Miguez S, Moreno María A, Fariña FA, et al. Understanding the global connection: Investigating the association between pork meat exports and trichinellosis[J]. Vet Parasitol, 2025, 338: 110509.
[4]	Zhang XZ, Wang ZQ, Cui J. Epidemiology of trichinellosis in the People’s Republic of China during 2009-2020[J]. Acta Trop, 2022, 229: 106388.
[5]	王中全, 崔晶. 旋毛虫属分类的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2002, 20(5): 310-314.
	Wang ZQ, Cui J. Recent progress in the taxonomy of the genus Trichinella[J]. Chin J Parasitol Parasit Dis, 2002, 20(5): 310-314. (in Chinese)
[6]	Zarlenga D, Thompson P, Pozio E. Trichinella species and genotypes[J]. Res Vet Sci, 2020, 133: 289-296.
[7]	国家市场监督管理总局, 国家标准化管理委员会. 旋毛虫诊断技术: GB/T 18642—2021[S]. 北京: 中国标准出版社, 2021: 1-28.
	State Administration for Market Regulation, National Standardization Administration. Diagnostic techniques for Trichinella spp.: GB/T 18642-2021[S]. Beijing: Standards Press of China, 2021: 1-28. (in Chinese)
[8]	Pozio E, Zarlenga D. International Commission on Trichinellosis: Recommendations for genotyping Trichinella muscle stage larvae[J]. Food Waterborne Parasitol, 2019, 15: e00033.
[9]	崔晶, 王中全. 旋毛虫与旋毛虫病的若干术语释义[J]. 中国寄生虫学与寄生虫病杂志, 2024, 42(4): 427-432.
	Cui J, Wang ZQ. Interpretations of some terminology of Trichinella and trichinellosis[J]. Chin J Parasitol Parasit Dis, 2024, 42(4): 427-432. (in Chinese)
[10]	Pozio E. Scientific achievements of the last 60 years: From a single to a multispecies concept of the genus Trichinella[J]. Vet Parasitol, 2021, 297: 109042.
[11]	Barlow A, Roy K, Hawkins K, et al. A review of testing and assurance methods for Trichinella surveillance programs[J]. Food Waterborne Parasitol, 2021, 24: e00129.
[12]	Bilska-Zając E, Thompson P, Rosenthal B, et al. Infection, genetics, and evolution of Trichinella: Historical insights and applications to molecular epidemiology[J]. Infect Genet Evol, 2021, 95: 105080.
[13]	Malone CJ, Oksanen A, Mukaratirwa S, et al. From wildlife to humans: The global distribution of Trichinella species and genotypes in wildlife and wildlife-associated human trichinellosis[J]. Int J Parasitol Parasites Wildl, 2024, 24: 100934.
[14]	Pozio E, Owen IL, Rosa GL, et al. Trichinella papuae n. sp. (Nematoda), a new non-encapsulated species from domestic and sylvatic swine of Papua New Guinea[J]. Int J Parasitol, 1999, 29(11): 1825-1839.
[15]	Pozio E, Foggin CM, Marucci G, et al. Trichinella zimbabwensis n.sp. (Nematoda), a new non-encapsulated species from crocodiles (Crocodylus niloticus) in Zimbabwe also infecting mammals[J]. Int J Parasitol, 2002, 32(14): 1787-1799.
[16]	Krivokapich SJ, Pozio E, Gatti GM, et al. Trichinella patagoniensis n. sp. (Nematoda), a new encapsulated species infecting carnivorous mammals in South America[J]. Int J Parasitol, 2012, 42(10): 903-910.
[17]	Sharma R, Thompson PC, Hoberg EP, et al. Hiding in plain sight: Discovery and phylogeography of a cryptic species of Trichinella (Nematoda: Trichinellidae) in wolverine (Gulo gulo)[J]. Int J Parasitol, 2020, 50(4): 277-287.
[18]	Zarlenga DS, Chute MB, Martin A, et al. A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella[J]. Int J Parasitol, 1999, 29(11): 1859-1867.
[19]	崔晶, 赵桂花, 王中全, 等. 应用多重PCR技术鉴定我国6个旋毛虫地理株的研究[J]. 中国寄生虫学与寄生虫病杂志, 2008, 26(2): 86-89.
	Cui J, Zhao GH, Wang ZQ, et al. Identification of six Trichinella isolates from China by multiplex PCR[J]. Chin J Parasitol Parasit Dis, 2008, 26(2): 86-89. (in Chinese)
[20]	Jiang P, Zhang X, Wang LA, et al. Survey of Trichinella infection from domestic pigs in the historical endemic areas of Henan Province, central China[J]. Parasitol Res, 2016, 115(12): 4707-4709.
[21]	Fu BQ, Liu MY, Yao CY, et al. Species identification of Trichinella isolates from China[J]. Vet Parasitol, 2009, 159(3/4): 214-217.
[22]	Zhang X, Han LL, Hong X, et al. Genotyping and phylogenetic position of Trichinella spiralis isolates from different geographical locations in China[J]. Front Genet, 2019, 10: 1093.
[23]	Pozio E, Pagani P, Marucci G, et al. Trichinella britovi etiological agent of sylvatic trichinellosis in the Republic of Guinea (West Africa) and a re-evaluation of geographical distribution for encapsulated species in Africa[J]. Int J Parasitol, 2005, 35(9): 955-960.
[24]	Nagano I, Wu Z, Matsuo A, et al. Identification of Trichinella isolates by polymerase chain reaction: Restriction fragment length polymorphism of the mitochondrial cytochrome c-oxidase subunit I gene[J]. Int J Parasitol, 1999, 29(7): 1113-1120.
[25]	Lobanov VA, Konecsni KA, Brad Scandrett W, et al. Identification of Trichinella taxa by ITS-1 amplicon next-generation sequencing with an improved resolution for detecting underrepresented genotypes in mixed natural infections[J]. Parasit Vectors, 2023, 16(1): 466.

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