为了解福建省牛片形吸虫感染情况及流行地区分布，于2023年5月—2024年5月在福建省9个市及1个综合实验区各选取2~3个县作为牛粪样采集点，按各县牛数量在全省牛总数中占比分配采样数量，每头牛取1份粪样。沉淀法收集牛粪样中的片形吸虫虫卵并进行形态学鉴定。各市（区）阳性粪样中随机选取10个虫卵，提取DNA，PCR扩增片形吸虫虫卵核糖体内转录间隔区2（ITS2）。采用SPSS20.0软件进行统计分析。共收集牛粪样502份，其中110份检出片形吸虫虫卵，感染率为21.9%（110/502）。检出的片形吸虫虫卵呈长椭圆形，淡黄褐色，卵一端有小盖。10个市（区）片形吸虫感染率由高到低依次是平潭区（52.8%，19/36）、福州市（42.5%，34/80）、龙岩市（37.9%，25/66）、宁德市（23.5%，8/34）、莆田市（16.0%，8/50）、漳州市（12.8%，6/47）、泉州市（10.3%，6/58）、厦门市（9.3%，3/32）、三明市（1.7%，1/59）、南平市（0，0/40），10个市（区）感染率差异有统计学意义（Fisher精确检验, P < 0.01）。9个市（区）阳性粪样均在550 bp处扩增出特异性条带。福建省牛片形吸虫在地理分布上较为分散，覆盖率较高，分布上呈现“西北高、东南低”的格局，其中以平潭区、福州市、龙岩市片形吸虫感染率较高，为高感染核心区域（感染率为> 36.0%~54.0%）。福建省牛片形吸虫流行地区分布广，家畜牛感染率较高。福建人群存在片形吸虫感染风险。

To investigate the prevalence and geographical distribution of Fasciola spp. in bovine in Fujian Province, 2 to 3 counties were selected from 9 cities and one comprehensive experimental area in Fujian Province from May 2023 to May 2024 as bovine stool sampling sites. Allocate sampling quantities based on the proportion of bovine in each county to the total number of bovine in the province, with one fecal sample taken from each bovine. The Fasciola eggs in bovine stool samples were collected using the precipitation method for morphological characterization. Ten Fasciola eggs were randomly sampled from positive fecal samples in each city (district), and genomic DNA was extracted from eggs for PCR amplification of ribosomal internal transcribed spacer region 2 (ITS2) gene. All statistical analyses were performed using the software SPSS 20.0. A total of 502 bovine stool samples were collected, and there 110 samples detected with Fasciola eggs, with 21.9% (110/502) prevalence of infections. The detected Fasciola eggs were elongated in shape, light yellow brown in color, and contained a small cap at one terminal. The Fasciola infection rates of the 10 cities (districts) from high to low are Pingtan District (52.8%, 19/36), Fuzhou City (42.5%, 34/80), Longyan City (37.9%, 25/66), Ningde City (23.5%, 8/34), Putian City (16.0%, 8/50), Zhangzhou City (12.8%, 6/47), Quanzhou City (10.3%, 6/58), Xiamen City (9.3%, 3/32), Sanming City (1.7%, 1/59), Nanping City (0/40), and there was a significant difference in the prevalence of Fasciola infections among the 10 cities (districts) (Fisher’s exact test, P < 0.01). Specific bands were amplified at 550 bp in Fasciola-infected bovine stool samples from 9 cities (districts). Spatial analysis showed that geographical distribution of Fasciola spp. was scattered in bovine in Fujian Province, with a high coverage rate and a landscape of “high in the northwestern region and low in the southeastern region”, with hotspots identified in Pingtan District, Fuzhou City, and Longyan City (prevalence of infection > 36.0% to 54.0%). The findings showed that the geographical distribution of Fasciola spp. is widespread in bovines in Fujian Province, with relatively high prevalence in livestock. There is a risk of infection with bovine fasciolosis among individuals living in Fujian Province.