收稿日期: 2024-10-28
修回日期: 2025-03-13
网络出版日期: 2025-06-18
基金资助
国家自然科学基金(82160400)
Investigation of chigger mites on the surface of murines and other small mammals in Liangshan Yi Autonomous Prefecture, Sichuan Province
Received date: 2024-10-28
Revised date: 2025-03-13
Online published: 2025-06-18
Supported by
National Natural Science Foundation of China(82160400)
目的 回顾性分析四川省凉山彝族自治州恙螨种类构成、分类名录和感染状况等,以期为该地区恙虫病及其媒介恙螨的监测和防控提供科学依据。 方法 2013—2020年间对凉山彝族自治州木里藏族自治县、冕宁县、昭觉县、盐源县、德昌县、会东县等6个县进行现场调查,不同生境常规采集鼠类等小兽体表恙螨并用霍氏液封制恙螨玻片标本,逐一鉴定到种。计算感染率(PM)、平均多度(MA)和感染度(MI)等感染指标,及丰富度指数(S)、Shannon-Wiener多样性指数(H’)、Pielou均匀度(E)和Simpson优势度指数(D)等群落指标。通过物种稀疏曲线、外推曲线和样本覆盖率判定采样充分程度。通过Spearson相关系数分析主要恙螨间的种间关系,采用双边网络分析宿主动物与恙螨间相互关系。 结果 从3目4科14属24种778只鼠类等小兽宿主体表鉴定出2科3亚科14属132种20 313只恙螨,其中有15种媒介或潜在媒介恙螨。纤恙螨属构成比最高(占75.3%,15 296/20 313)。凉山全境小兽体表恙螨的总PM 为65.2%,总MA为26.11只/兽,总MI 为 40.07只/兽,S = 32,H’ = 3.20,E = 0.65,D = 0.92;样本覆盖率为99.9%。恙螨感染指标随海拔梯度升高呈逐渐降低趋势,群落指标随海拔梯度增高呈先增高后降低趋势。在132种恙螨中,5种优势恙螨构成比为52.4%,5种优势恙螨和5中主要媒介恙螨两两之间呈不同程度的正相关关系(r = 0.19~0.87,P < 0.01)。双边网络分析显示,一种宿主可以承载多种恙螨,一种恙螨可以寄生多种宿主。 结论 凉山彝族自治州恙螨种类丰富,物种多样性高,宿主感染普遍且感染严重,不同海拔地带恙螨感染和恙螨群落结构存在差异;主要恙螨之间有同时选择同一宿主和种间共存倾向;该州纤恙螨属大量存在,有15种媒介恙螨共存,且多数恙螨宿主特异性低,增加了该州恙虫病持续传播的潜在风险。
刘晨曦 , 郭宪国 , 吕艳 , 尹鹏吾 , 宋文宇 , 彭培英 , 相蓉 , 陈雁翎 , 李贝 . 四川省凉山彝族自治州鼠类等小兽体表恙螨的调查研究[J]. 中国寄生虫学与寄生虫病杂志, 2025 , 43(3) : 385 -394 . DOI: 10.12140/j.issn.1000-7423.2025.03.013
Objective To retrospectively analyze the species composition, list of classification, and infection prevalence of chigger mites in Liangshan Yi Autonomous Prefecture, Sichuan Province, so as to provide insights into monitoring and prevention and control of scrub typhus and its vector chigger mites in the prefecture. Methods Field surveys were conducted in six counties of Muli Tibetan Autonomous County, Mianning County, Zhaojue County, Yanyuan County, Dechang County, and Huidong County in Liangshan Yi Autonomous Prefecture from 2013 to 2020. Chigger mites were collected from the surface of murines and small mammals in different habitats, enveloped with Hoyer’s medium to generate slide specimens of chigger mites, and individually characterized to species. The prevalence (PM), mean abundance (MA), and intensity of chigger mite infections (MI) were calculated, and the community indicators of chigger mites were estimated, including richness index (S), Shannon Wiener diversity index (H’), Pielou evenness (E), and Simpson dominance index (D). The adequacy of sampling was determined using species rarefaction curves, extrapolation curves, and sample coverage. In addition, the interspecific relationships among main chigger mites were examined using Spearson correlation coefficients, and the interrelationships between host animals and chigger mites were evaluated using bilateral network analysis. Results A total of 20 313 chigger mites were identified from the surface of 778 murines and other small mammal hosts belonging to 24 species, 14 genera, 4 families and 3 orders, including 132 species (15 vector or potential vector chigger mites), 14 genera, 3 subfamilies and 2 families, and Leptotrombidium was the dominant genus (75.3%, 15 296/20 313). The gross PM, MA, MI, S, H’, E and D of chigger mites were 65.2%, 26.11 mites/animal, 40.07 mites/animal, 32, 3.20, 0.65, and 0.92 on the surface of small mammals across Liangshan, with sample coverage of 99.9%. The PM, MA and MI of chigger mites appeared a tendency towards a decline with the rise in elevation, and the community indicators of chigger mites appeared a tendency towards a rise followed by a decline with the rise in elevation. Of 132 species of chigger mites, the constituent ratio of five dominant species was 52.4%, and there were positive correlations with varying degrees among 5 dominant chigger mites and 5 main vector chigger mites (r = 0.19-0.87, P < 0.01). Bilateral network analysis revealed that one host harbored multiple species of chigger mites and one species of chigger mites was parasitized in multiple species of hosts. Conclusion Chigger mites are abundant in species and high in species diversity in Liangshan Yi Autonomous Prefecture. The prevalence of chigger mite infections is common and high in hosts, and the prevalence of chigger mite infections and community structure of chigger mites vary in elevation. The main chigger mites have a tendency towards choosing the same host and interspecific coexistence in Liangshan, and Leptotrombidium genus is widespread in Liangshan. There are 15 species of vector chigger mites, and most present a low specificity to hosts, which increases the potential risk of continuous transmission of scrub typhus in Liangshan.
| [1] | Nielsen DH, Robbins RG, Rueda LM. Annotated world checklist of the Trombiculidae and Leeuwenhoekiidae (1758-2021) (Acari : Trombiculoidea), with notes on nomenclature, taxonomy, and distribution[J]. Zootaxa, 2021, 4967(1): 1-243. |
| [2] | 刘小虎, 任天广. 中国恙螨新种及新纪录名录[J]. 中国热带医学, 2022, 22(6): 512-516. |
| Liu XH, Ren TG. The list of new species and new record chigger mites in China[J]. China Trop Med, 2022, 22(6): 512-516. (in Chinese) | |
| [3] | Moniuszko H, Makol J. Chigger mites (Actinotrichida : Parasitengona, Trombiculidae) of Poland. An updated distribution and hosts[J]. Ann Parasitol, 2014, 60(2): 103-117. |
| [4] | Stekolnikov AA. A checklist of chigger mites (Acariformes : Trombiculidae) of Southeast Asia[J]. Zootaxa, 2021, 4913(1): zootaxa.4913.1.1. |
| [5] | 黎家灿. 中国恙螨: 恙虫病媒介和病原体研究[M]. 广州: 广东科技出版社, 1997: 1-570. |
| Li JC. Chigger mites in China: Study on vectors and pathogens of tsutsugamushi disease[M]. Guangzhou: Guangdong Science & Technology Press, 1997: 1-570. (in Chinese) | |
| [6] | Takahashi M, Morita K, Tsuji O, et al. Seasonal development of Leptotrombidium akamushi (Acari : Trombiculidae) under field temperatures[J]. J Med Entomol, 1995, 32(6): 843-846. |
| [7] | Daniel M, Stekolnikov AA, Hakimitabar M, et al. Chigger mites (Acari, Trombiculidae) parasitizing small mammals in the Eastern Hindu Kush and some other Afghan areas[J]. Parasitol Res, 2010, 107(5): 1221-1233. |
| [8] | Matthee S, Stekolnikov AA, van der Mescht L, et al. The diversity and distribution of chigger mites associated with rodents in the South African savanna[J]. Parasitology, 2020, 147(9): 1038-1047. |
| [9] | Axman WR, Brummer JJ. Chigger mite infestation[J]. J Am Podiatric Med Assoc, 2003, 93(5): 399-401. |
| [10] | Yu XJ, Tesh RB. The role of mites in the transmission and maintenance of Hantaan virus (Hantavirus : Bunyaviridae)[J]. J Infect Dis, 2014, 210(11): 1693-1699. |
| [11] | Walker DH, Mendell NL. A scrub typhus vaccine presents a challenging unmet need[J]. NPJ Vaccines, 2023, 8(1): 11. |
| [12] | Chen KY, Roe RM, Ponnusamy L. Biology, systematics, microbiome, pathogen transmission and control of chiggers (Acari : Trombiculidae, Leeuwenhoekiidae) with emphasis on the United States[J]. Int J Environ Res Public Health, 2022, 19(22): 15147. |
| [13] | Wang YC, Zhang CT, Gao J, et al. Spatiotemporal trends of hemorrhagic fever with renal syndrome (HFRS) in China under climate variation[J]. Proc Natl Acad Sci USA, 2024, 121(4): e2312556121. |
| [14] | Li ZJ, Xin HL, Sun JL, et al. Epidemiologic changes of scrub typhus in China, 1952-2016[J]. Emerg Infect Dis, 2020, 26(6): 1091-1101. |
| [15] | Luo YZ, Lv H, Yan HC, et al. Meteorological change and hemorrhagic fever with renal syndrome epidemic in China, 2004-2018[J]. Sci Rep, 2022, 12(1): 20037. |
| [16] | Yue YJ, Ren DS, Liu XB, et al. Spatio-temporal patterns of scrub typhus in mainland China, 2006-2017[J]. PLoS Negl Trop Dis, 2019, 13(12): e0007916. |
| [17] | 彭培英, 徐蕾, 王谷仙, 等. 1952—1989年和2006—2017年中国大陆恙虫病流行及时空分布特征[J]. 中国人兽共患病学报, 2022, 38(9): 818-823, 829. |
| Peng PY, Xu L, Wang GX, et al. Epidemiological characteristics and spatiotemporal distribution of scrub typhus in mainland China in 1952-1989 and 2006-2017[J]. Chin J Zoonoses, 2022, 38(9): 818-823, 829. (in Chinese) | |
| [18] | Zhang Y, Zhang MY, Qin Y, et al. Epidemiological analysis and risk prediction of scrub typhus from 2006 to 2021 in Sichuan, China[J]. Front Public Health, 2023, 11: 1177578. |
| [19] | 汪娟, 刘伦光, 袁珩, 等. 2006—2021年四川省斑疹伤寒流行特征分析[J]. 预防医学情报杂志, 2023, 39(11): 1337-1344. |
| Wang J, Liu LG, Yuan H, et al. Epidemiology characteristics of typhus in Sichuan Province from 2006 to 2021[J]. J Prev Med Inf, 2023, 39(11): 1337-1344. (in Chinese) | |
| [20] | 汪娟, 冯玉亮, 周兴余, 等. 2018—2022年四川省流行性出血热流行特征分析[J]. 预防医学情报杂志, 2024, 40(8): 923-931. |
| Wang J, Feng YL, Zhou XY, et al. Epidemic characteristics of epidemic hemorrhagic fever in Sichuan Province from 2018 to 2022[J]. J Prev Med Inf, 2024, 40(8): 923-931. (in Chinese) | |
| [21] | 徐伟, 郑玄, 欧文, 等. 四川凉山州地质灾害灾情特征与主要致灾类型[J]. 中国地质灾害与防治学报, 2024, 35(5): 78-89. |
| Xu W, Zheng X, Ou W, et al. Characteristics of losses of geological disasters and major disaster types in Liangshan Prefecture, Sichuan Province[J]. Chin J Geol Hazard Control, 2024, 35(5): 78-89. (in Chinese) | |
| [22] | Li B, Guo XG, Zhao CF, et al. Infestation of chigger mites on Chinese mole shrew, Anourosorex squamipes, in Southwest China and ecological analysis[J]. Parasite, 2022, 29: 39. |
| [23] | Ding F, Jiang WL, Guo XG, et al. Infestation and related ecology of chigger mites on the Asian house rat (Rattus tanezumi) in Yunnan Province, southwest China[J]. Korean J Parasitol, 2021, 59(4): 377-392. |
| [24] | Peng PY, Guo XG, Ren TG, et al. Species diversity of ectoparasitic chigger mites (Acari : Prostigmata) on small mammals in Yunnan Province, China[J]. Parasitol Res, 2016, 115(9): 3605-3618. |
| [25] | 魏辅文. 中国兽类分类与分布[M]. 北京: 科学出版社, 2022: 27-28, 75-200, 205-300. |
| Wei FW. Taxonomy and distribution of mammals in China[M]. Beijing: Science Press, 2022: 27-28, 75-200, 205-300. (in Chinese) | |
| [26] | Smith AT, Xie Y. A guide to the mammals of China[M]. Changsha: Hunan Education Publishing House, 2009: 7-8, 31-183. (in Chinese) |
| (史密斯•安德鲁, 解焱. 中国兽类野外手册[M]. 长沙: 湖南教育出版社, 2009: 7-8, 31-183.) | |
| [27] | 黄文几, 徐士菊. 中国啮齿类[M]. 上海: 复旦大学出版社, 1995: 44-90, 110-207, 220-242. |
| Huang WJ, Xu SJ. Rodents in China[M]. Shanghai: Fudan Press, 1995: 44-90, 110-207, 220-242. (in Chinese) | |
| [28] | Stekolnikov AA. Leptotrombidium (Acari : Trombiculidae) of the world[J]. Zootaxa, 2013, 3728: 1-173. |
| [29] | Guo XG, Qian TJ, Meng XY, et al. Preliminary analysis of chigger communities associated with house rats (Rattus flavipectus) from six counties in Yunnan, China[J]. Syst Appl Acarol, 2006, 11(1): 13. |
| [30] | Chao A, Jost L. Coverage-based rarefaction and extrapolation: Standardizing samples by completeness rather than size[J]. Ecology, 2012, 93(12): 2533-2547. |
| [31] | Hsieh TC, Ma KH, Chao A. iNEXT: An R package for rarefaction and extrapolation of species diversity (Hill numbers)[J]. Meth Ecol Evol, 2016, 7(12): 1451-1456. |
| [32] | Chao A, Gotelli NJ, Hsieh TC, et al. Rarefaction and extrapolation with hill numbers: A framework for sampling and estimation in species diversity studies[J]. Ecol Monogr, 2014, 84(1): 45-67. |
| [33] | 徐满厚, 刘敏, 翟大彤, 等. 植物种间联结研究内容与方法评述[J]. 生态学报, 2016, 36(24): 8224-8233. |
| Xu MH, Liu M, Zhai DT, et al. A review of contents and methods used to analyze various aspects of plant interspecific associations[J]. Acta Ecol Sin, 2016, 36(24): 8224-8233. (in Chinese) | |
| [34] | Ali Abd Al-Hameed K. Spearman’s correlation coefficient in statistical analysis[J]. Int J Nonlinear Anal, 2022, 13(1): 3249-3255. |
| [35] | Pesenti C, Navone SE, Guarnaccia L, et al. The genetic landscape of human glioblastoma and matched primary cancer stem cells reveals intratumour similarity and intertumour heterogeneity[J]. Stem Cells Int, 2019, 2019: 2617030. |
| [36] | 谷岳礼, 刘雅琼, 周兴余. 2014—2018年四川省流行性出血热流行特征分析[J]. 寄生虫病与感染性疾病, 2020, 18(1): 1-4. |
| Gu YL, Liu YQ, Zhou XY. Epidemiological characteristics of epidemic hemorrhagic fever in Sichuan Province from 2014 to 2018[J]. Parasit Infect Dis, 2020, 18(1): 1-4. (in Chinese) | |
| [37] | 曹一鸥, 周兴余, 袁珩, 等. 2005—2022年四川省斑疹伤寒流行特征分析[J]. 中华卫生杀虫药械, 2023, 29(2): 122-125. |
| Cao YO, Zhou XY, Yuan H, et al. Epidemiological characteristics of typhus in Sichuan Province from 2005 to 2022[J]. Chin J Hyg Insectic Equip, 2023, 29(2): 122-125. (in Chinese) | |
| [38] | Mi XC, Feng G, Hu YB, et al. The global significance of biodiversity science in China: An overview[J]. Natl Sci Rev, 2021, 8(7): nwab032. |
| [39] | 白学礼, 闫立民, 马英, 等. 宁夏恙螨名录[J]. 地方病通报, 2009, 24(2): 11-12. |
| Bai XL, Yan LM, Ma Y, et al. Catalogue of chigger mites in Ningxia Hui Autonomous Region[J]. Endem Dis Bull, 2009, 24(2): 11-12. (in Chinese) | |
| [40] | 周淑姮, 韩腾伟, 王加熊, 等. 福建省部分地区野栖鼠体表寄生恙螨的物种多样性调查[J]. 中国人兽共患病学报, 2021, 37(6): 511-519. |
| Zhou SH, Han TW, Wang JX, et al. Investigation of chigger mite species diversity parasitizing wild rodents in some areas of Fujian Province[J]. Chin J Zoonoses, 2021, 37(6): 511-519. (in Chinese) | |
| [41] | 刘国平, 李东力, 陈春田, 等. 我国东北三省的恙螨[J]. 中国媒介生物学及控制杂志, 2003, 14(6): 444-446. |
| Liu GP, Li DL, Chen CT, et al. Chigger mites from three provinces of Northeast China[J]. Chin J Vector Biol Control, 2003, 14(6): 444-446. (in Chinese) | |
| [42] | 徐伟, 铁永波, 郑玄, 等. 凉山州地质灾害发育特征与时空分布规律[J]. 沉积与特提斯地质, 2024, 44(3): 478-492. |
| Xu W, Tie YB, Zheng X, et al. Development characteristics and temporal-spatial distribution of geological hazards in Liangshan Prefecture[J]. Sediment Geol Tethyan Geol, 2024, 44(3): 478-492. (in Chinese) | |
| [43] | Chen YL, Guo XG, Ren TG, et al. Infestation and distribution of chigger mites on Chevrieri’s field mouse (Apodemus chevrieri) in Southwest China[J]. Int J Parasitol Parasites Wildl, 2022, 17: 74-82. |
| [44] | Chen YL, Guo XG, Ding F, et al. Infestation of oriental house rat (Rattus tanezumi) with chigger mites varies along environmental gradients across five provincial regions of southwest China[J]. Int J Environ Res Public Health, 2023, 20(3): 2203. |
| [45] | 曾剑, 徐晴晗, 张宇, 等. 中国西南地区百年气温的时空演变特征[J]. 成都信息工程大学学报, 2022, 37(4): 412-421. |
| Zeng J, Xu QH, Zhang Y, et al. Temporal and spatial characteristics of temperature in southwest China during the past century[J]. J Chengdu Univ Inf Technol, 2022, 37(4): 412-421. (in Chinese) | |
| [46] | 李瀚, 韩琳, 贾志军, 等. 中国西南地区地面平均相对湿度变化分析[J]. 高原山地气象研究, 2016, 36(4): 42-47. |
| Li H, Han L, Jia ZJ, et al. The changes of the average relative humidity in southwest China[J]. Plateau Mt Meteor Res, 2016, 36(4): 42-47. (in Chinese) | |
| [47] | 尹鹏吾. 西南地区小兽体表革螨物种多样性、区系特征及相关生态研究[D]. 贵阳: 贵州大学, 2024: 167-168. |
| Yin PW. Diversity, faunal characteristics and related ecology of gamasid mites (Acari:Mesostigmata:Gamasina) on small mammals in southwest China[D]. Guiyang: Guizhou University, 2024: 167-168. (in Chinese) | |
| [48] | Chaisiri K, Gill AC, Stekolnikov AA, et al. Ecological and microbiological diversity of chigger mites, including vectors of scrub typhus, on small mammals across stratified habitats in Thailand[J]. Anim Microbiome, 2019, 1(1): 18. |
| [49] | Xiang R, Ren TG, Guo XG. Research history and progress of six vector chigger species of scrub typhus in China[J]. Saa, 2022: 1841-1856. |
| [50] | 吴光华, 姜志宽, 王莉, 等. 我国恙虫病媒介恙螨的依据与鉴定[J]. 中华卫生杀虫药械, 2013, 19(4): 286-292. |
| Wu GH, Jiang ZK, Wang L, et al. Accordance and identification of vector chigger mites of tsutsugamushi disease in China[J]. Chin J Hyg Insectic Equip, 2013, 19(4): 286-292. (in Chinese) | |
| [51] | Zhang YZ, Yuan JF, Yang XL, et al. A novel hantavirus detected in Yunnan red-backed vole (Eothenomys miletus) in China[J]. J Gen Virol, 2011, 92(Pt 6): 1454-1457. |
| [52] | Men XY, Guo XG, Dong WG, et al. Ectoparasites of Chevrier’s field mouse, Apodemus chevrieri, in a focus of plague in southwest China[J]. Med Vet Entomol, 2007, 21(3): 297-300. |
| [53] | 周瑜, 任天广. 云南省恙虫病媒介恙螨区系研究进展[J]. 中国热带医学, 2023, 23(5): 550-555. |
| Zhou Y, Ren TG. Fauna of scrub typhus vector chigger mites in Yunnan Province[J]. China Trop Med, 2023, 23(5): 550-555. (in Chinese) |
/
| 〈 |
|
〉 |