目的 鉴定丹顶鹤感染前殖吸虫的种类并分析其核糖体DNA内转录间隔区（ITS）。方法 从黑龙江省扎龙自然保护区丹顶鹤的泄殖腔采集前殖吸虫，PCR扩增前殖吸虫ITS序列并测序，测序结果在NCBI进行BLAST比对。采用基因重复序列计算器查找重复序列，MegAlign软件进行序列相似性分析，MEGA 7.0、Clustal X和Paup软件进行序列对齐，采用最大简约法构建系统进化树。结果 在丹顶鹤体内共采集到5条前殖吸虫，PCR扩增共获得5条ITS序列，其中3条长1 240 bp（GenBank登录号：PQ634967，PQ634968，PQ634969），与楔形前殖吸虫（Prosthogonimus cuneatus）（GenBank登录号：OQ344776）的序列相似性为98.2%，鉴定为楔形前殖吸虫；2条长为1 183 bp（GenBank登录号：PP956934，PP956935），与透明前殖吸虫（P. pellucidus）（GenBank登录号：KP192732）的序列相似性为97.7%，鉴定为透明前殖吸虫。楔形前殖吸虫和透明前殖吸虫的5.8S和ITS2无重复序列，ITS1则分别有16、17个重复序列。系统进化树结果显示，本研究中的楔形前殖吸虫先与寄生于乌鸫、绿头鸭的楔形前殖吸虫（捷克）聚为一个小分支，再与本研究中的透明前殖吸虫聚为一个分支。结论 扎龙自然保护区丹顶鹤体感染的前殖吸虫为楔形前殖吸虫和透明前殖吸虫，其ITS1分别有16、17个重复序列。楔形前殖吸虫和透明前殖吸虫与来自捷克的楔形前殖吸虫的亲缘关系较近。

Objective To identify the species of Prosthogonimus in Grus japonensis and analyze the ribosomal DNA internal transcribed spacer (ITS) gene sequence. Methods Prosthogonimus samples were collected from the G. japonensis cloaca in Zhalong National Nature Reserve, Heilongjiang Province. The ITS gene of Prosthogonimus was amplified using PCR assay, and the amplified product was sequenced. The sequencing results were aligned with sequences recorded in the NCBI database using the BLAST tool. Repetitive sequences were identified using the Repeat Masker tool, and sequence similarity analysis was performed using the MegAlign software. Sequence alignments were conducted using the MEGA 7.0, Clustal X, and Paup software, and a phylogenetic tree was created using the maximum parsimony method. Results A total of 5 Prosthogonimus specimens were collected from G. japonensis, and five ITS gene sequences were obtained. Three sequences (GenBank accession numbers: PQ634967, PQ634968 and PQ634969) were 1 240 bp in size and showed 98.2% sequence similarity with P. cuneatus (GenBank accession number: OQ344776), which were identified as P. cuneatus. The other two sequences (GenBank accession numbers: PP956934 and PP956935) were 1 183 bp in size and showed 97.7% sequence similarity with P. pellucidus (GenBank accession number: KP192732), which were identified as P. pellucidus. There were no repetitive sequences in 5.8S or ITS2 genes of P. cuneatus and P. pellucidus, and there were 16 and 17 repetitive sequences the ITS1 gene of P. cuneatus and P. pellucidus, respectively. Phylogenetic analysis revealed that the P. cuneatus from this study was firstly clustered with P. cuneatus from Turdusmerula and Anasplatyrhynchos (Czech Republic isolate) into a subclade, and then clustered with P. pellucidus from this study into a clade. Conclusion The Prosthogonimus species infecting G. japonensis in the Zhalong National Nature Reserve are P. cuneatus and P. pellucidus, with 16 and 17 repetitive sequences in the P. cuneatus and P. pellucidus ITS1, respectively. P. cuneatus and P. pellucidus share a closer phylogenetic relationship with the Czech Republic isolate of P. cuneatus.