收稿日期: 2024-03-18
修回日期: 2024-06-01
网络出版日期: 2025-01-14
基金资助
2021年福建省卫生健康科技计划项目(2021QNA037)
Genetic identification on Meotrchis orientalis infection in human populations of Pucheng County, Fujian Province
Received date: 2024-03-18
Revised date: 2024-06-01
Online published: 2025-01-14
Supported by
Fujian Provincial Health Technology Project(2021QNA037)
为了解福建省浦城县人群东方次睾吸虫感染情况，补充该虫的生物学信息。收集2016—2020年浦城县国家肝吸虫病监测点发现的东方次睾吸虫感染者粪样。采用改良加藤厚涂片法（Kato-Katz法，一粪两检）检查东方次睾吸虫虫卵；提取粪样DNA，PCR扩增东方次睾吸虫核糖体DNA内转录间隔区（ITS）序列并测序。计算两种检测方法的阳性率，采用卡方检验进行比较。共收集东方次睾吸虫感染者粪样13份，其中Kato-Katz法检出东方次睾吸虫阳性粪样11份；10份粪样经PCR扩增出262 bp的目的条带，与东方次睾吸虫ITS序列（MK482055）的一致性达到99.6%。Kato-Katz法和PCR法的阳性率分别为11/13和10/13，两者差异无统计学意义（χ2 = 0.25，P > 0.05）。本研究结果显示，浦城县人群存在东方次睾吸虫感染，易与华支睾吸虫感染混淆。本研究使用的ITS序列能够区分东方次睾吸虫和华支睾吸虫，可以作为东方次睾吸虫的基因标志物。
陈云虹 , 谢贤良 , 陈宝建 , 陈方美 , 余文武 . 福建省浦城县人群感染东方次睾吸虫的基因鉴定[J]. 中国寄生虫学与寄生虫病杂志, 2024 , 42(6) : 810 -812 . DOI: 10.12140/j.issn.1000-7423.2024.06.019
To understand the infection status of Meotrchis orientalis among the population in Pucheng County, Fujian Province and supplement the biological information of M. orientalis. Fecal samples of individuals infected with M. orientalis reported from the national clonorchiasis surveillance site of Pucheng County from 2016 to 2020 were collected. Modified Kato-Katz thick smear method (two slides per sample) was used to examine the eggs of M. orientalis. The DNA was extracted from fecal samples. The internal transcribed spacer (ITS) sequence of M. orientalis ribosomal DNA was amplified by PCR and sequenced. The positive rates of 2 methods were calculated and compared by Chi-square test. A total of 13 fecal samples of individuals infected with M. orientalis were collected. Among them, 11 tested positive using Kato-Katz method; Target bands at 262 bp were amplified using PCR method in 10 fecal samples, which showed a 99.6% identity to the ITS sequence of M. orientalis (MK482055). The positive rates of Kato-Katz method and PCR method were 11/13 and 10/13, respectively, with no statistically significant difference (χ2 = 0.25, P > 0.05). The results indicated that there was an infection of M. orientalis among the population in Pucheng County, which was easily confused with Clonorchis sinensis infection. The ITS sequence used in this study can distinguish between M. orientalis and C. sinensis, and can be used as a genetic marker for M. orientalis.
Key words： Meotrchis orientalis; Infection; Molecular identification; Pucheng County
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