目的 了解浙江省华支睾吸虫感染流行现状。 方法 2013—2017年，每年选择浙江省1个曾有华支睾吸虫病例报告的县（市、区）为监测点，每个监测点选择3个乡（镇）开展人群监测，各乡（镇）选择至少100位3周岁以上当地居民采集静脉血，使用酶联免疫吸附试验（ELISA）检测华支睾吸虫抗体，计算监测对象的抗体阳性率；对2013—2015年的监测对象开展影响华支睾吸虫感染率因素的问卷调查。2018—2022年，每年以宁波市宁海县为固定监测点、另选7~9个县（市、区）为流动监测点，每个监测点按地理位置划分为东、西、南、北、中等5个片区，每个片区抽取1个乡（镇）的1个行政村，整群抽取3周岁以上当地居民200人采集粪样（> 30 g），使用改良加藤厚涂片法检测华支睾吸虫虫卵，计算检测对象感染率。2013—2017年，每年在监测点的自然水体环境中采集淡水鱼；2018—2022年，每年在固定监测点和随机一个流动监测点的自然水体环境中采集淡水鱼，鉴定鱼种后采用压片法检查囊蚴感染情况。2018—2022年，每年在固定监测点和监测中间宿主的流动监测点采集猫、犬或猪粪样，用改良加藤厚涂片法检测华支睾吸虫虫卵。用SPSS 19.0进行数据统计分析，感染率的比较采用卡方检验。 结果 2013—2017年浙江省共监测1 516人，抗体阳性率为2.51%（38/1 516）；2018—2022年共监测52 626人，粪样中均未检出华支睾吸虫虫卵，感染率为0（0/52 626）。各监测点人群抗体阳性率以磐安县最高（6.00%，18/300），不同监测点间人群抗体阳性率差异有统计学意义（χ² = 21.212，P < 0.01）。监测人群中3~17岁年龄组抗体阳性率最高（8.82%，6/68），不同年龄组间抗体阳性率差异有统计学意义（χ² = 13.105，P < 0.05）。2013—2022年中间宿主囊蚴感染率为12.56%（476/3 791），感染率最高的为2018年的宁海县（33.47%，84/251），不同监测点间中间宿主感染率差异有统计学意义（χ² = 242.727，P < 0.01）。逆鱼囊蚴感染率最高（75.00%，21/28），不同鱼种感染率差异有统计学意义（χ² = 174.750，P < 0.01）。保虫宿主感染率为1.17%（3/257）。 结论 浙江省人群华支睾吸虫感染水平较低，但中间宿主和保虫宿主仍有感染，具有潜在流行风险，需加强监测防治和健康教育。

Objective To ascertain the endemic status of Clonorchis sinensis in Zhejiang Province. Methods From 2013 to 2017, one county (city, district) which had clonorchiasis cases reported before in Zhejiang Province was selected as the regular surveillance site and 3 townships in each site were selected for monitoring each year. No less than 100 permanent residents over age 3 in each township were selected as monitored objects. Venous blood samples were collected, and serum C. sinensis antibody was detected by enzyme-linked immunosorbent assay (ELISA). The positive rate of antibodies in the monitored objects was calculated. And questionnaire surveys were conducted on the factors affecting the infection of C. sinensis from 2013 to 2015. From 2018 to 2022, Ningbo City was selected as the fixed surveillance site and 7-9 counties (cities, districts) were selected as the mobile surveillance sites. Each surveillance site was divided into 5 areas as geographically at east, west, south, north and centre. In each area, one administrative village was randomly selected from one township for cluster sampling of no less than 200 permanent residents over age 3. Fecal samples (over 30 g) were collected for examining C. sinensis eggs using the modified Kato-Katz thick smear method. The infection rate of the monitored object was calculated. Freshwater fish from natural water were collected in regular surveillance sites from 2013 to 2017 and in the fixed surveillance site and a mobile surveillance site from 2018 to 2022. The infection of encysted metacercaria was detected by pressing method after identified the species. From 2018 to 2022, the fecal samples of cats, dogs and pigs were collected in surveillance sites which monitoring the intermediate hosts, and examined C. sinensis eggs using the modified Kato-Katz thick smear method. SPSS 19.0 was used for data statistical analysis. Infection rates were compared between groups using the chi-squared test. Results From 2013 to 2017, a total of 1 516 people were examined in Zhejiang Province and antibody positive rate was 2.51% (38/1 516). A total of 52 626 people were examined from 2018 to 2022. No C. sinensis eggs were found in fecal samples and the infection rate was 0 (0/52 626). The highest antibody positive rate of 6.00% (18/300) was seen in Panan County. The antibody positive rate in different surveillance sites was statistically significant (χ² = 21.212, P < 0.01). The highest antibody positive rate of 8.82% (6/68) was seen in the age 3-17 group. The antibody positive rate in different age groups was statistically significant (χ² = 13.105, P < 0.05). The infection rate of C. sinensis in freshwater fish from 2013 to 2022 were 12.56% (476/3 791). The highest infection rate of 33.47% (84/251) was seen in Ninghai County in 2018. The infection rate in different surveillance sites was statistically significant (χ² = 242.727, P < 0.01). The highest infection rate of 75.00% (21/28) was seen in Acanthobrama simoni. The infection rate in different fish species was statistically significant (χ² = 174.750, P < 0.01). The infection rate of reservoir host was 1.17% (3/257). Conclusion The infection rate of C. sinensis in human population was considerably low in surveillance sites in Zhejiang Province, but the infected intermediate hosts and reservoir hosts still remained, which poses a potential epidemic risk and requires strengthened surveillance, prevention and health education.