收稿日期: 2023-09-04
修回日期: 2023-11-07
网络出版日期: 2024-03-12
基金资助
上海市加强公共卫生体系建设三年行动计划(2023—2025)重点学科项目(GWⅥ-11.1-09);国家自然科学基金(82372283);国家科技重大专项(2018ZX10713001-004)
Infection and molecular characterization of Enterocytozoon bieneusi and Cyclospora cayetanensis in pet dogs and cats in Shanghai
Received date: 2023-09-04
Revised date: 2023-11-07
Online published: 2024-03-12
Supported by
Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai (2023—2025) Key Discipline Project(GWⅥ-11.1-09);National Natural Science Foundation of China(82372283);National Science and Technology Major Program of China(2018ZX10713001-004)
目的 了解上海市宠物犬、猫毕氏肠微孢子虫和卡耶塔环孢子虫感染情况及分子特征，评估人兽共患传播风险。方法 2021年11月—2022年6月，采集上海市某宠物医院犬和猫的新鲜粪样，提取粪样基因组DNA，巢式PCR扩增毕氏肠微孢子虫核糖体内转录间隔区（ITS）和卡耶塔环孢子虫小亚基核糖体RNA（SSU rRNA）序列，阳性产物经双向测序后在GenBank数据库中进行BLAST比对。使用MEGA 11.0软件基于邻接法构建系统进化树。结果 本研究共采集145份粪样（犬粪样99份、猫粪样46份），毕氏肠微孢子虫总阳性率为4.1%（6/145），卡耶塔环孢子虫总阳性率为4.8%（7/145）；犬的毕氏肠微孢子虫和卡耶塔环孢子虫阳性率分别为3.0%（3/99）和6.1%（6/99)，猫的毕氏肠微孢子虫和卡耶塔环孢子虫阳性率分别为6.5%（3/46）和2.2%（1/46）。6条毕氏肠微孢子虫ITS序列与人源基因型A（GenBank登录号：MK982500）的序列一致性均为100%，归在组1；7条卡耶塔环孢子虫SSU rRNA序列与人源卡耶塔环孢子虫（GenBank登录号：KJ569533）的序列一致性均为100%。系统进化树分析结果显示，毕氏肠微孢子虫分离株与已报道的人源毕氏肠微孢子虫基因型A分离株归为同一分支，卡耶塔环孢子虫的分离株与已报道的人源卡耶塔环孢子虫分离株归为同一分支。结论 上海市宠物犬、猫中存在毕氏肠微孢子虫和卡耶塔环孢子虫感染，均为人兽共感染型虫株。
秦源 , 刘华 , 王雅雪 , 张璟 , 苏雅馨 , 曹建平 , 沈玉娟 . 上海市宠物犬和猫毕氏肠微孢子虫和卡耶塔环孢子虫感染情况及分子特性研究[J]. 中国寄生虫学与寄生虫病杂志, 2024 , 42(1) : 63 -68 . DOI: 10.12140/j.issn.1000-7423.2024.01.009
Objective To investigate the infection and molecular characteristics of Enterocytozoon bieneusi and Cyclospora cayetanensis in pet dogs and cats in Shanghai and assess the zoonotic potential. Methods Fresh fecal samples were collected from dogs and cats at a pet hospital in Shanghai from November 2021 to June 2022. Genomic DNA was extracted from the fecal samples, and nested PCR was used to amplify the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) of E. bieneusi and the small subunit ribosomal RNA (SSU rRNA) sequence of C. cayetanensis. The positive products were subjected to bidirectional sequencing and sequence alignment by BLAST in the GenBank database. The phylogenetic tree was constructed using MEGA 11.0 software based on the neighbor-joining method. Results A total of 145 fecal samples were collected (99 from dogs and 46 from cats). The positive rates of E. bieneusi and C. cayetanensis were 4.1% (6/145) and 4.8% (7/145), respectively. The positive rates of E. bieneusi and C. cayetanensis in dogs were 3.0% (3/99) and 6.1% (6/99), respectively, while those in cats were 6.5% (3/46) and 2.2% (1/46), respectively. The ITS sequences of 6 E. bieneusi were 100% identical to human genotype A (GenBank accession number: MK982500), and the SSU rRNA sequences of 7 C. cayetanensis were 100% identical to human C. cayetanensis (GenBank accession number: KJ569533). The results of the phylogenetic tree analysis showed that all the E. bieneusi isolates belonged to the same branch as the reported human E. bieneusi genotype A isolates and fell into group 1. All the C. cayetanensis isolates belonged to the same branch as the reported human C. cayetanensis isolates. Conclusion E. bieneusi and C. cayetanensis infections were detected in pet dogs and cats in Shanghai, all of which were caused by zoonotic parasite strains.
Key words： Enterocytozoon bieneusi; Cyclospora cayetanensis; Pets; Zoonotic disease
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