目的 调查大兴安岭地区家犬寄生虫感染情况，为当地人兽共患寄生虫病的防控提供参考。方法 于2020年9月在大兴安岭地区加北村、白桦村、东山村、图强镇、幸福村和古源镇等6个自然村的养犬户院内收集新鲜家犬粪便，提取粪样总DNA，使用顶复门（Apicomplexa）、阿米巴属（Amoeba）、双滴虫目（Diplomonadida）、动质体目（Kinetoplastida）、毛滴虫属（Parabasalia）、线形动物门（Nematoda）、扁形动物门（Platyhelminthes）和小孢子虫目（Microsporidia）等8个分类学群的13对寄生虫通用引物进行PCR扩增，扩增产物经高通量测序后获得目的基因片段，产物序列在NCBI数据库中进行比对分析以鉴定虫种，统计家犬粪便的寄生虫检出率。结果 202份家犬粪便中有37份检出寄生虫DNA，寄生虫总检出率为18.32%。共计检出7个门15个科的19种寄生虫，不同虫种检出率差异具有统计学意义（χ² = 69.488，P < 0.05）。检出原虫13种，蠕虫6种。其中，以原虫眼虫门动质体纲Parabodonida科的Parabodo caudatus检出率最高，为6.44%（13/202）。在37份检出寄生虫DNA的样品中，混合检出阳性占比为32.43%（12/37），其中检出1种寄生虫的占72.97%（27/37），同时检出2种、3种、4种寄生虫的分别占27.03%（10/37）、0.27%（1/37）和0.27%（1/37）。家犬粪便寄生虫DNA检出率以加北村最高，为37.70%（23/61），其次为图强镇，检出率为36.36%（20/55），白桦村未检出（0/13）。不同采样点的检出率差异具有统计学意义（χ² = 19.717，P < 0.05）。结论 大兴安岭地区家犬体内存在多种寄生虫感染，且部分地区检出率较高，当地居民存在一定的犬源性寄生虫感染风险。

Objective To investigate the parasitic infection in domestic dogs in Da Hinggan Ling area, and provide reference for the prevention and control of local zoonotic parasitic diseases. Methods In September 2020, fresh domestic dog feces were collected from dog owner’s household yard of 6 natural villages including Jiabei Village, Baihua Village, Dongshan Village, Tuqiang Town, Xingfu Village and Guyuan Town in the Da Hinggan Ling area, and total DNA was extracted from the fecal samples. PCR amplification was conducted using 13 pairs of parasite universal primers targeting 8 taxonomy groups including Apicomplexa, Amoeba, Diplomonadida, Kinetoplastida, Parabasalia, Nematoda, Platyhelminthes and Microsporidia, and the target gene fragments were obtained after high-throughput sequencing of the amplified products. The product sequences were compared and analyzed in the NCBI database to identify the parasite species, and the detection rate of parasites in domestic dog feces was calculated. Results Parasite DNA were detected in 37 of 202 domestic dog feces, and the total detection rate was 18.31%. A total of 19 species of parasites which belongs to 15 families from 7 phyla were detected, and the detection rate of different species were significantly different (χ² = 69.488, P < 0.05). Thirteen protozoa species and 6 helminth species were detected. The detection rate of Parabodo caudatus in the family Parabodonidae was the highest at 6.44% (13/202). Among the 37 samples in which parasite DNA was detected, the mixed positive ratio was 32.43% (12/37), of which 72.97% (27/37) detected 1 type of parasite, and 2 types, 3 kinds and 4 kinds accounted for 27.03% (10/37), 0.27% (1/37) and 0.27% (1/37) respectively. Jiabei Village had the highest detection rate of parasite DNA in domestic dog feces, which was 37.70% (23/61), followed by Tuqiang Town, with a detection rate of 36.36% (20/55), and Baihua Village, which was not detected (0/13). There was a statistically significant difference in the detection rate of different sampling sites (χ² = 19.717, P < 0.05). Conclusion In Da Hinggan Ling area, domestic dogs were found infected with multiple parasites, showing considerably higher positive rate detected in some parts of the area, thus, there exists risk of infection caninee-source parasites to local residents.