收稿日期: 2021-03-31
修回日期: 2021-05-21
网络出版日期: 2021-11-10
基金资助
国家自然科学基金(3206130239);福建省漳州市自然科学基金(ZZ2017J09);福建省漳州市重大科技项目(ZZ2017ZD05)
Intermediate host population and infection status of Paragonimus in southeastern Youxi County, Fujian Province
Received date: 2021-03-31
Revised date: 2021-05-21
Online published: 2021-11-10
Supported by
National Natural Science Foundation(3206130239);Zhangzhou Natural Science Foundation of Fujian(ZZ2017J09);Zhangzhou Key Project of Science and Technology Plan(ZZ2017ZD05)
2015—2020年,从福建省尤溪县东南部华兰溪流域溪尾乡的埔宁村、溪尾村和汤川乡的赤墓村、珠建村等4个村,每村选1条水量较少、1条水量较多的水沟作为调查点,采集淡水螺、蟹,对螺和蟹进行形态学分类,分离螺类并殖吸虫尾蚴和蟹类囊蚴并进行鉴定。用从蟹类分离的卫氏并殖吸虫和斯氏并殖吸虫囊蚴各80条分别经口感染幼犬1只,感染后60 d起粪检查找虫卵,分别于感染后80、100 d剖杀幼犬,收集成虫,对成虫进行形态学分类。共查获淡水螺类3种,共7 405只。其中,卫氏并殖吸虫第一中间宿主螺1种,为放逸短沟蜷;斯氏并殖吸虫第一中间宿主螺2种,分别为待定种拟钉螺和待定种洱海螺。埔宁村（拟钉螺）、溪尾村（拟钉螺）和赤墓村（洱海螺）等3个村的螺类斯氏并殖吸虫尾蚴的感染率分别0.39%（6/1 546）、0.17%（2/1 179）和0.26%（5/1 898）;珠建村的放逸短沟蜷检出卫氏并殖吸虫尾蚴,感染率为0.11%（1/917）。共查获蟹类5种319只,均为并殖吸虫第二中间宿主,分别为福建华溪蟹、永安博特溪蟹、尤溪博特溪蟹、福建南海溪蟹和待定种华南溪蟹。溪蟹并殖吸虫囊蚴总感染率为25.71%（82/319）,平均感染度为7.34个囊蚴/蟹,感染指数为3.09。其中埔宁村的尤溪博特溪蟹、福建华溪蟹的斯氏并殖吸虫囊蚴感染率为41.89%（31/74）：溪尾村的待定种华南溪蟹、福建华溪蟹的斯氏并殖吸虫囊蚴感染率为22.67%（17/75）;赤墓村的福建华溪蟹、永安博特溪蟹、福建南海溪蟹的斯氏并殖吸虫囊蚴感染率为30.0%（21/70）。珠建村的福建华溪蟹、永安博特溪蟹、福建南海溪蟹的卫氏并殖吸虫囊蚴感染率为13.00%（13/100）。囊蚴感染犬后分别获得卫氏并殖吸虫和斯氏并殖吸虫成虫12、10条。福建省尤溪县东南部存在多种并殖吸虫第一中间宿主螺类和第二中间宿主蟹类,为中度感染风险的并殖吸虫病自然疫源地。
罗鋆 , 陈及清 , 江典伟 , 周培聪 , 蔡茂荣 , 程由注 . 福建省尤溪县东南部并殖吸虫中间宿主种群及其感染情况[J]. 中国寄生虫学与寄生虫病杂志, 2021 , 39(5) : 646 -651 . DOI: 10.12140/j.issn.1000-7423.2021.05.022
From 2015 to 2020, four villages in southeast Youxi County, Fujian Province (Puning Village and Xiwei Village in Xiwei Town, Chimu Village and Zhujian Village in Tangchuan Town) were selected as survey sites. Freshwater snails and crabs were collected in one shallow and one deep gully from each village and were identified by morphological characteristic. Paragonimus cercariae from snails and cysticerci from crabs were isolated and identified. Two puppies were fed with 80 metacercariae of P. westermani or P. skrjabini isolated from crabs. The eggs were examined in feces starting 60 days after infection, and the puppies were dissected to collect adult worms at 80 days and 100 days after infection. The adult worms were identified by morphological characteristics. We collected 7 405 freshwater snails from three species: Semisulcospira liberlina, the first intermediate host of P. westermani, and undefined Triculini and Erhaia, the first intermediate host of P. skriabini. The infection rates of P. skrjabini cercariae in Puning Village (Triculini), Xiwei Village (Triculini) and Chimu Village (Erhaia) were 0.39% (6/1 546), 0.17% (2/1 179) and 0.26% (5/1 898), respectively. The infection rate of P. westermani cercariae in S. liberlina in Zhujian Village was 0.11% (1/917). A total of 319 crabs from five species （S. fukienense, B. yonganense, B. youxiense, N. fujianense, and undefined Huananpotamon） were collected. All species were the second intermediate hosts of Paragonimus. The total infection rate of freshwater crabs was 25.71% (82/319). The average infection intensity was 7.34 metacercariae per crab, and the infection index was 3.09. The infection rate of P. skrjabini metacercariae in B. youxiense and S. fukienense in Puning Village was 41.89% (31/74). The infection rate of P. skrjabini metacercariae in undefined Huananpotamon and S. fukienense in Xiwei Village was 22.67% (17/75). The infection rate of P. skrjabini metacercariae in S. fukienense, B. yonganense, and N. fujianense in Chimu Village was 30.0% (21/70). The infection rate of P. westermani metacercariae in S. fukienense, B. yonganense, and N. fujianense in Zhujian Village was 13.00% (13/100). A total of 12 and 10 adult worms of P. westermani and P. skrjabini, respectively, were collected from puppies infected with metacercariae, respectively. Many species of Paragonimus first intermediate host snails and Paragonimus second intermediate host crabs were found in the southeast Youxi County, Fujian Province, which is a moderate-risk area for Paragonimus infection.
Key words： Paragonimus; Intermediate host; Snail; Crab; Infection rate; Youxi County
| [1] | Lin CX, Li YS. Epidemic status of paragonimiasis in Tangchuan Township, Fujian Province[J]. Parasitoses Infect Dis, 2004, 2(2):83-84. (in Chinese) |
| [1] | ( 林陈鑫, 李友松. 福建省尤溪县汤川乡肺吸虫病流行情况调查[J]. 寄生虫病与感染性疾病, 2004, 2(2):83-84.) |
| [2] | Cheng YZ, Li LS, Lin GH, et al. Survey on the foci of paragonimiasis in Youxi, Yongtai and Pinghe counties of Fujian Province[J]. Chin J Parasitol Parasit Dis, 2010, 28(6):406-410. (in Chinese) |
| [2] | ( 程由注, 李莉莎, 林国华, 等. 福建省尤溪、永泰与平和县并殖吸虫病疫源地调查[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(6):406-410.) |
| [3] | Cheng YZ, Lin GH, Li YS. Two new species of freshwater crabs (Decapoda ∶ Potamidae) serving as intermediate hosts of Paragonimus in Fujian, China[J]. Chin J Parasitol Parasit Dis, 2010, 28(4):241-245. (in Chinese) |
| [3] | ( 程由注, 林国华, 李友松. 并殖吸虫宿主淡水蟹类两新种记述(十足目 ∶ 溪蟹科)[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(4):241-245.) |
| [4] | Gao N, Cui YY, Wang SB, et al. Two newspecies and the molecular phylogeography of thefreshwster crab genus Bottapotamorn (Crustacea ∶ Decapoda ∶ Brachyura ∶ Potamidae)[J]. Peer J, 2019: 7980. (in Chinese) |
| [5] | Liu YY, Zhang WZ, Wang YX. Medical malacology[M]. Bejing: Ocean Press, 1993: 39-60. (in Chinese) |
| [5] | ( 刘月英, 张文珍, 王耀先. 医学贝类学[M]. 北京: 海洋科学出版社, 1993: 39-60.) |
| [6] | Kang ZB. Two new species of Triculini snails harbouring cercariae of Paragonimus skrjabini[J]. J Hubei Med Coll, 1983, 4(1):106-108. (in Chinese) |
| [6] | ( 康在彬. 携带斯氏并殖吸虫尾蚴的拟钉螺两新种[J]. 湖北医学院学报, 1983, 4(1):106-108.) |
| [7] | Liu YY, Zhang WZ. On new genus and species of freshwater snails harbouring cercariae of lung flukes from China[J]. Acta Zootaxonomica Sin, 1979, 4(2):132-136. (in Chinese) |
| [7] | ( 刘月英, 张文珍. 携带肺吸虫尾蚴的淡水螺类一新属二新种记述[J]. 动物分类学报, 1979, 4(2):132-136.) |
| [8] | Cheng YZ, Wu XP, Li LS. A new species of pseudobythinella (Mesogastropoda, Pomatiopsidae) from China[J]. Acta Zootaxonomica Sin, 2007, 32(4):896-899. (in Chinese) |
| [8] | ( 程由注, 吴小平, 李莉莎. 拟小豆螺属一新种记述(中腹足目, 盖螺科)[J]. 动物分类学报, 2007, 32(4):896-899.) |
| [9] | Li YS, Cheng YZ. A new species of fresh water crab infected with metacercariae of Paragonimus: the genus Huananpotamon tangi sp. Nov[J]. Chin J Zoonoses, 2008, 24(2):125-127. (in Chinese) |
| [9] | ( 李友松, 程由注. 感染并殖吸虫囊蚴唐氏华南溪蟹新种(Huananpotamon tangi sp. nov)记述(十足目 ∶ 溪蟹科)[J]. 中国人兽共患病学报, 2008, 24(2):125-127.) |
| [10] | Dai AY, Zoology of China (Arthropod, Crustacea, Decapoda, Parathephusidae, Potamidae)[M]. Beijing: Sciene Press, 1999: 125-143. (in Chinese) |
| [10] | ( 戴爱云. 中国动物志(节肢动物门, 甲壳纲, 十足目, 束腹蟹科、溪蟹科)[M]. 北京: 科学出版社, 1999: 125-143.) |
| [11] | Dai AY, Chen GX. On the fresh-water crabs from Fujian Province[J]. Acta Zool Sin, 1979, 25(3):243-249. (in Chinese) |
| [11] | ( 戴爱云, 陈国孝. 福建省淡水蟹类的调查研究[J]. 动物学报, 1979, 25(3):243-249.) |
| [12] | Cheng YZ, Lin JX, Luo XQ. A new species of crab of the genus Malayopotamon (Decapoda ∶ Isolapotamidae)[J]. Acta Zootaxonomica Sin, 1993, 18(4):412-416. (in Chinese) |
| [12] | ( 程由注, 林金祥, 罗信驱. 马来溪蟹属一新种(十足目 ∶ 石蟹科)[J]. 动物分类学报, 1993, 18(4):412-416.) |
| [13] | Lin GH, Cheng YZ, Chen SH. A new species of the genus Nanhaipotamon (Decapoda ∶ Potamidae) serving as intermediate host of Paragonimus skrjbini[J]. Chin J Parasitol Parasit Dis, 2013, 31(1):39-42. (in Chinese) |
| [13] | ( 林国华, 程由注, 陈韶红. 斯氏并殖吸虫宿主南海溪蟹属一新种记述[J]. 中国寄生虫学与寄生虫病杂志, 2013, 31(1):39-42. |
| [14] | Fan PF, Liu JR, Chen DX, et al. Distribution of Paragonimus westermani in south Anhui[J]. J Bengbu Med Coll, 1980, 5(2):1-6. (in Chinese) |
| [14] | ( 樊培方, 刘家荣, 陈黛霞, 等. 安徽南部卫氏并殖吸虫分布[J]. 蚌埠医学院学报, 1980, 5(2):1-6.) |
| [15] | Shen YP. Soiid Paragonimiasis [M]. 2nd Ed. Beijing: Health Press, 2008: 5-28. (in Chinese) |
| [15] | ( 沈一平. 实用肺吸虫病学[M]. 2版. 北京: 卫生出版社, 2008: 5-28.) |
| [16] | Cheng YZ. Ctudies on the relationship between the infection of intermediate hosts of Paragonimus and ecological environment[J]. Chin J Parasitol Parasit Dis, 1999, 17(4):212-214. (in Chinese) |
| [16] | ( 程由注. 并殖吸虫中间宿主的感染与其生态环境的关系[J]. 中国寄生虫学与寄生虫病杂志, 1999, 17(4):212-214. |
| [17] | Li YS, Lin JX, Cheng YZ, et al. Study on reasons and changes of infection rate in 10 epidemic areas of paragonimiasis in Fujian Province[J]. Chin J Parasit Dis Control, 1999(4):275-277. (in Chinese) |
| [17] | ( 李友松, 林金祥, 程由注, 等. 福建省并殖吸虫病疫区感染率的变化及其原因探讨[J]. 中国寄生虫病防治杂志, 1999(4):275-277) |
| [18] | Cheng YZ, Xu LS, Chen BJ, et al. Survey on the current ststus of important human parasitic infections in Fujian Province, China[J]. Chin J Parasitol Parasit Dis, 2005, 23(5):283-287. (in Chinese) |
| [18] | ( 程由注, 许龙善, 陈宝健, 等. 福建省人体重要寄生虫感染调查分析[J]. 中国寄生虫学与寄生虫病杂志, 2005, 23(5):283-287.) |
| [19] | Cai MR, Luo J, Ai L, et al. Investigation on the infection of Paragonimus skrjabini in intermediate hosts in northern Fujian[J]. Chin J Parasitol Parasit Dis, 2020, 38(1):102-109.(in Chinese) |
| [19] | ( 蔡茂荣, 罗鋆, 艾琳, 等. 福建北部地区斯氏并殖吸虫疫源地宿主感染情况分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1):102-109.) |
| [20] | Cai MR, Li YS, Cheng YZ. Paragonimus & paragonimiasis in Fujian Province[M]. Xiamen: Xiamen University Press, 2021:81-84. |
| [20] | ( 蔡茂荣, 李友松, 程由注. 福建省肺吸虫与肺吸虫病[M]. 厦门: 厦门大学出版社, 2021: 81-84.) |
/
| 〈 |
|
〉 |