收稿日期: 2020-12-22
修回日期: 2021-01-21
网络出版日期: 2021-07-05
基金资助
国家自然科学基金(81830066);国家自然科学基金(U1803282);国家自然科学基金(31860703)
Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar County, Xinjiang
Received date: 2020-12-22
Revised date: 2021-01-21
Online published: 2021-07-05
Supported by
National Natural Sciecne Foundation of China(81830066);National Natural Sciecne Foundation of China(U1803282);National Natural Sciecne Foundation of China(31860703)
目的 了解新疆察布查尔县多房棘球绦虫流行区中间宿主——小型啮齿类动物的感染和分布情况,确定多房棘球绦虫的中间宿主种类,为当地制定防控措施提供科学依据。方法 2019年选择加尕斯台乡、琼博乐乡、四师和都拉塔口岸等4个调查点,使用板夹、弓形夹捕捉小型啮齿类动物。根据外形特点并结合头骨形态鉴定小型啮齿类动物的种类。取捕获的小型啮齿类动物疑似感染的肝脏分别进行HE染色及PCR方法扩增并测序确定其感染情况。不同地区和不同种群小型啮齿类动物感染率的比较采用χ2检验。 结果 本调查共捕获小型啮齿类动物595只,经形态学鉴定分属7个种,其中普通田鼠464只、小林姬鼠84只、柽柳沙鼠14只、大沙鼠14只、坦氏鼹形田鼠8只、灰仓鼠6只和红尾沙鼠5只。67份疑似感染的肝脏样品可见多房棘球蚴病灶,HE染色结果显示,病灶组织可见多房棘球蚴原头节;PCR扩增结果和序列比对表明,该67只小型啮齿类动物均为多房棘球蚴感染,总感染率为11.3%。各种的感染率分别为普通田鼠12.8%（57/464）、小林姬鼠3.6%（3/84）、柽柳沙鼠1/14、大沙鼠2/14、坦氏鼹形田鼠2/8、红尾沙鼠1/5和灰仓鼠1/6。普通田鼠的感染率与小林姬鼠比较差异有统计学意义（P < 0.05）;普通田鼠与其他5种小型啮齿类动物之间感染率差异无统计学意义（ P > 0.05）。4个调查点中,以琼博乐乡捕获的小型啮齿类动物的感染率最高,为12.3%（49/398）。 结论 新疆察布查尔县所捕获的小型啮齿类动物主要为普通田鼠和小林姬鼠,前者多房棘球蚴感染率最高。
郭宝平 , 郭刚 , 张莉 , 向晶晶 , 王小平 , 任远 , 齐文静 , 张慧 , 李军 , 张文宝 , 汪海燕 . 新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查[J]. 中国寄生虫学与寄生虫病杂志, 2021 , 39(3) : 327 -332 . DOI: 10.12140/j.issn.1000-7423.2021.03.006
Objective To investigate the infection and distribution of Echinococcus multilocularis in small rodents, the intermediate hosts, in Chabuchaer County, Xinjiang, and to identify the intermediate host species of the parasite, providing scientific basis for formulating local control measures. Methods Small rodents were captured with plate clamps and bow clips in four selected investigation sites, including Jiagasitai town, Qiongbole town, Sishi and Dulata Port. Suspected infection in liver tissues was confirmed by HE staining and PCR amplification. The PCR products were sequenced. The species of small rodents were identified according to their physical appearance and skull morphology. Theχ2 test was used to compare the infection rate in different regions and in different rodent populations. Results A total of 595 small rodents were captured, which belonged to 7 species, including 464 Microtus spp., 84 Apodemus sylvaticus, 14 Meriones tamariscinus Pallas, 14 Rhombomys sopimus Pallas, 8 Ellobius trancrei, 6 Cricetulus migratorius Pallas and 5 Meriones erythrourus Pallas. In 67 liver samples of suspected infection, lesions of E. multilocularis metacestodes were observed. HE staining showed that the protoscoleces of E. multilocularis metacestodes were visible in the lesion tissues. PCR amplification and sequence alignment showed that all the 67 small rodents were infected with metacestodes of E. multilocularis, with a total infection rate of 11.3%. All the seven species of rodents were infected, with infection rates of 12.8% (57/464), 3.6% (3/84), 1/14, 2/14, 2/8, 1/5 and 1/6 for Microtus spp, A. sylvaticus, M. tamariscinus Pallas, R. sopimus Pallas, E. trancrei, M. serythrourus Pallas and C. migratorius Pallas, respectively. Chi-square analysis showed that there was a significant difference in the infection rate between Microtus spp. and A. sylvaticus (P < 0.05). The infection rate of Microtus spp. did not significantly differ from that of the other species (P > 0.05). Of the 4 investigation sites, the Qiongbole town showed the highest infection rate (12.3%, 49/398). Conclusion The small rodents captured in Chabuchaer County were mainly Microtus spp. and A. sylvaticus, with the former species showing the highest infection rate of metacestodes.
| [1] | Deplazes P, Rinaldi L, Alvarez Rojas CA, et al. Global distribution of alveolar and cystic echinococcosis[J]. Adv Parasitol, 2017,95:315-493. |
| [2] | Zhang MY, Wang LY, Guan YY, et al. Meta-analysis on severity of hydatidosis[J]. Chin J Parasitol Parasit Dis, 2018,36(2):l56-160. (in Chinese) |
| [2] | ( 张梦媛, 王立英, 官亚宜, 等. 棘球蚴病严重程度的Meta分析[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(2):156-160.) |
| [3] | Torgerson PR, Keller K, Magnotta M, et al. The global burden of alveolar echinococcosis[J]. PLoS Negl Trop Dis, 2010,4(6):e722. |
| [4] | Wen H, Vuitton L, Tuxun T, et al. Echinococcosis: advances in the 21st century[J]. Clin Microbiol Rev, 2019,32(2):e00075-e00018. |
| [5] | Zhang WB, Zhang ZZ, Shi BX, et al. Vaccination of dogs against Echinococcus granulosus the cause of cystic hydatid disease in humans[J]. J Infect Dis, 2006,194(7):966-974. |
| [6] | Giraudoux P, Raoul F, Pleydell D, et al. Drivers of Echinococcus multilocularis transmission in China: small mammal diversity, landscape or climate?[J]. PLoS Negl Trop Dis, 2013,7(3):e2045. |
| [7] | Lin YG, Hong LX. The biology and geographical distribution of Echinococcus multilocularis infection in China[J]. Endem Dis Bull, 1991,6(2):117-127. (in Chinese) |
| [7] | ( 林宇光, 洪凌仙. 我国多房棘球绦虫病的病原生物学及其在我国的地理分布[J]. 疾病预防控制通报, 1991,6(2):117-127.) |
| [8] | Gesy K, Hill JE, Schwantje H, et al. Establishment of a European-type strain of Echinococcus multilocularis in Canadian wildlife[J]. Parasitology, 2013,140(9):1133-1137. |
| [9] | Grishailla I. Observation on the curative effect of albendazole on hepatic hydatid disease[J]. Chin Pract J Rural Dr, 2016,23(6):57-58. (in Chinese) |
| [9] | ( 古丽沙依拉·英斯.. 阿苯达唑治疗肝包虫病疗效观察[J]. 中国实用乡村医生杂志, 2016,23(6):57-58.) |
| [10] | Wang Z, Wang X, Liu X. Echinococcosis in China, a review of the epidemiology of Echinococcus spp[J]. Ecohealth, 2008,5(2):115-126. |
| [11] | Tang CT, Quian YC, Kang YM, et al. Study on the ecological distribution of alveolar Echinococcus in Hulunbeier Pasture of Inner Mongolia, China[J]. Parasitology, 2004,128(Pt 2):187-194. |
| [12] | Wang C, He H, Li M, et al. Parasite species associated with wild plateau pika (Ochotona curzoniae) in southeastern Qinghai Province, China[J]. J Wildl Dis, 2009,45(2):288-294. |
| [13] | Wang W, Wu Y, Wu JG, et al. The occurence ofEchinococcus multilocularis leukart, 1863 in fox and wolf in Tacheng district, Xinjiang[J]. Endem Dis Bull, 1989,4(2):8-11. (in Chinese) |
| [13] | ( 王伟, 吴勇, 吴季高, 等. 新疆塔城地区多房棘球绦虫调查: 国内狼体多房棘球绦虫新记录[J]. 地方病通报, 1989,4(2):8-11.) |
| [14] | Holt DW, Hanns C, O’Hara T, et al. New distribution records of Echinococcus multilocularis in the brown lemming from Barrow, Alaska, USA[J]. J Wildl Dis, 2005,41(1):257-259. |
| [15] | Wang SY, Yang GY. Annals of Xinjiang rodents[M]. Urumqi: Xinjiang People’s Publishing House, 1983: 8-28. (in Chinese) |
| [15] | ( 王思博, 杨赣源. 新疆啮齿动物志 [M]. 乌鲁木齐: 新疆人民出版社, 1983: 8-28.) |
| [16] | Pleydell DR, Yang YR, Danson FM, et al. Landscape composition and spatial prediction of alveolar echinococcosis in southern Ningxia, China[J]. PLoS Negl Trop Dis, 2008,2(9):e287. |
| [17] | Wu C, Zhang W, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in Western China[J]. Parasit Vectors, 2017,10(1):265. |
| [18] | Giraudoux P, Pleydell D, Raoul F, et al. Transmission ecology of Echinococcus multilocularis: what are the ranges of parasite stability among various host communities in China?[J]. Parasitol Int, 2006,55:S237-S246. |
| [19] | Wen H, Zhang YL, Bart J, et al. Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog[J]. Chin J Parasitol Parasit Dis, 2006,24(1):10-13. (in Chinese) |
| [19] | ( 温浩, 张亚楼, Bart J, 等. 犬体内细粒棘球绦虫和多房棘球绦虫的混合感染[J]. 中国寄生虫学与寄生虫病杂志, 2006,24(1):10-13.) |
| [20] | Wang DM, He RF, Gong S, et al. Prevalence of echinococcosis in Nyingchi City[J]. Chin J Parasitol Parasit Dis, 2018,36(1):75-79. (in Chinese) |
| [20] | ( 王栋民, 何瑞峰, 贡桑曲珍, 等. 林芝市棘球蚴病流行情况[J]. 中国寄生虫学与寄生虫病杂志, 2018,36(1):75-79.) |
| [21] | Van Kesteren F, Mastin A, Mytynova B, et al. Dog ownership, dog behaviour and transmission of Echinococcus spp. in the Alay Valley, southern Kyrgyzstan[J]. Parasitology, 2013,140(13):1674-1684. |
| [22] | Liccioli S, Giraudoux P, Deplazes P, et al. Wilderness in the ‘City’ revisited: different urbes shape transmission of Echinococcus multilocularis by altering predator and prey communities[J]. Trends Parasitol, 2015,31(7):297-305. |
| [23] | Guo BP. Study on correlation between pathogenic differences and mitochondrial genetic markers in Echinococcus multilocularis[D]. Shihezi, China: Shihezi University, 2019: 2-6. (in Chinese) |
| [23] | ( 郭宝平. 多房棘球绦虫致病差异与线粒体遗传标志相关性的研究[D]. 石河子: 石河子大学, 2019: 2-6.) |
| [24] | Raoul F, Deplazes P, Rieffel D, et al. Predator dietary response to prey density variation and consequences for cestode transmission[J]. Oecologia, 2010,164(1):129-139. |
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